Product: MYL4 Antibody
Catalog: DF8302
Description: Rabbit polyclonal antibody to MYL4
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Rabbit, Dog, Xenopus
Mol.Wt.: 22 kDa; 22kD(Calculated).
Uniprot: P12829
RRID: AB_2841584

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Horse(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
MYL4 Antibody detects endogenous levels of total MYL4.
RRID:
AB_2841584
Cite Format: Affinity Biosciences Cat# DF8302, RRID:AB_2841584.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ALC 1; ALC1; AMLC; Atrial/embryonic alkali myosin light chain; embryonic muscle/atrial isoform; GT 1; GT1; MLC 1; MLC1; MYL 4; Myl4; MYL4_HUMAN; Myosin atrial/fetal muscle light chain; Myosin light chain 1; Myosin light chain 1 embryonic muscle/atrial isoform; Myosin light chain 4 alkali atrial embryonic; Myosin light chain 4; Myosin light chain alkali GT 1 isoform; Myosin light chain alkali GT-1 isoform; Myosin light polypeptide 4 alkali atrial embryonic; Myosin light polypeptide 4; PRO1957;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MAPKKPEPKKEAAKPAPAPAPAPAPAPAPAPEAPKEPAFDPKSVKIDFTADQIEEFKEAFSLFDRTPTGEMKITYGQCGDVLRALGQNPTNAEVLRVLGKPKPEEMNVKMLDFETFLPILQHISRNKEQGTYEDFVEGLRVFDKESNGTVMGAELRHVLATLGEKMTEAEVEQLLAGQEDANGCINYEAFVKHIMSG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Xenopus
100
Rabbit
100
Bovine
0
Sheep
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P12829 As Substrate

Site PTM Type Enzyme
T66 Phosphorylation
Y75 Phosphorylation
Y132 Phosphorylation
S146 Phosphorylation
T149 Phosphorylation
K165 Acetylation
S196 Phosphorylation

Research Backgrounds

Function:

Regulatory light chain of myosin. Does not bind calcium.

Subunit Structure:

Myosin is a hexamer of 2 heavy chains and 4 light chains.

Research Fields

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Organismal Systems > Circulatory system > Cardiac muscle contraction.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.