MPP1 Antibody - #DF8276

(3)  
Product: MPP1 Antibody
Catalog: DF8276
Description: Rabbit polyclonal antibody to MPP1
Application: WB IF/ICC
Reactivity: Human
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 52 kDa; 52kD(Calculated).
Uniprot: Q00013
RRID: AB_2841565

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(89%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
MPP1 Antibody detects endogenous levels of total MPP1.
RRID:
AB_2841565
Cite Format: Affinity Biosciences Cat# DF8276, RRID:AB_2841565.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

55 kDa erythrocyte membrane protein; AAG 12; AAG12; Aging associated gene 12; DXS552; DXS552E; EM55_HUMAN; EMP 55; EMP55; Erythrocyte membrane protein p55; Membrane protein; Membrane protein palmitoylated 1 55kDa; Membrane protein palmitoylated 1; Membrane protein, palmitoylated 1, 55kDa; Migration related gene 1; MPP 1; MPP1; MRG 1; MRG1; OTTHUMP00000026054; OTTHUMP00000196176; p55; palmitoylated 1; Palmitoylated erythrocyte membrane protein; Palmitoylated membrane protein 1; PEMP;

Immunogens

Immunogen:
Uniprot:

Q00013(EM55_HUMAN) >>Visit HPA database.

Gene(ID):
MPP1(4354)
Expression:
Q00013 EM55_HUMAN:

Ubiquitous.

Sequence:
MTLKASEGESGGSMHTALSDLYLEHLLQKRSRPEAVSHPLNTVTEDMYTNGSPAPGSPAQVKGQEVRKVRLIQFEKVTEEPMGITLKLNEKQSCTVARILHGGMIHRQGSLHVGDEILEINGTNVTNHSVDQLQKAMKETKGMISLKVIPNQQSRLPALQMFMRAQFDYDPKKDNLIPCKEAGLKFATGDIIQIINKDDSNWWQGRVEGSSKESAGLIPSPELQEWRVASMAQSAPSEAPSCSPFGKKKKYKDKYLAKHSSIFDQLDVVSYEEVVRLPAFKRKTLVLIGASGVGRSHIKNALLSQNPEKFVYPVPYTTRPPRKSEEDGKEYHFISTEEMTRNISANEFLEFGSYQGNMFGTKFETVHQIHKQNKIAILDIEPQTLKIVRTAELSPFIVFIAPTDQGTQTEALQQLQKDSEAIRSQYAHYFDLSLVNNGVDETLKKLQEAFDQACSSPQWVPVSWVY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Rabbit
100
Chicken
89
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q00013 As Substrate

Site PTM Type Enzyme
T2 Acetylation
S13 Phosphorylation
S19 Phosphorylation
Y22 Phosphorylation
K29 Ubiquitination
S37 Phosphorylation
T42 Phosphorylation
T44 Phosphorylation
Y48 Phosphorylation
T49 Phosphorylation
S52 Phosphorylation
S57 Phosphorylation
K76 Ubiquitination
K91 Ubiquitination
S110 Phosphorylation
K147 Ubiquitination
S154 Phosphorylation
K180 Ubiquitination
K197 Ubiquitination
K212 Acetylation
K212 Ubiquitination
S220 Phosphorylation
S241 Phosphorylation
S243 Phosphorylation
K247 Ubiquitination
Y251 Phosphorylation
Y255 Phosphorylation
K258 Ubiquitination
K281 Methylation
K299 Ubiquitination
K309 Ubiquitination
Y316 Phosphorylation
K323 Ubiquitination
S324 Phosphorylation
Y331 Phosphorylation
S335 Phosphorylation
S344 Phosphorylation
K371 Ubiquitination
K374 Ubiquitination
K386 Ubiquitination
T407 Phosphorylation
K417 Ubiquitination
Y429 Phosphorylation

Research Backgrounds

Function:

Essential regulator of neutrophil polarity. Regulates neutrophil polarization by regulating AKT1 phosphorylation through a mechanism that is independent of PIK3CG activity (By similarity).

PTMs:

Palmitoylated.

Subcellular Location:

Cell membrane>Lipid-anchor. Cell projection>Stereocilium.
Note: Colocalizes with WHRN at stereocilium tip during hair cell development (By similarity). Colocalizes with MPP5 in the retina, at the outer limiting membrane (OLM) (By similarity). Colocalizes with WHRN in the retina, at the outer limiting membrane (OLM), outer plexifirm layer (OPL), basal bodies and at the connecting cilium (CC) (By similarity). Colocalizes with NF2 in non-myelin-forming Schwann cells (PubMed:19144871).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Heterodimer with MPP5. Interacts with DLG5 and NF2. Interacts (via guanylate kinase-like domain) with WHRN (via third PDZ domain).

Family&Domains:

Belongs to the MAGUK family.

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