EBP50 Antibody - #DF8271
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Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF8271, RRID:AB_2841560.
Fold/Unfold
EBP 50; EBP50; Ezrin radixin moesin binding phosphoprotein 50; Ezrin-radixin-moesin-binding phosphoprotein 50; Na(+)/H(+) exchange regulatory cofactor NHE RF; Na(+)/H(+) exchange regulatory cofactor NHE-RF1; Na+/H+ exchange regulatory co factor; NHERF 1; NHERF; NHERF-1; NHERF1; NHRF1_HUMAN; NPHLOP2; Regulatory cofactor of Na(+)/H(+) exchanger; Slc9a3r1; Sodium hydrogen exchanger regulatory factor 1; Sodium-hydrogen exchanger regulatory factor 1; Sodium/hydrogen exchanger regulatory factor 1; Solute carrier family 9 (sodium/hydrogen exchanger) member 3 regulator 1; Solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1; Solute carrier family 9 isoform 3 regulatory factor 1; Solute carrier family 9 isoform A3 regulatory factor 1; Solute carrier family 9 member 3 regulator 1;
Immunogens
Detected in liver, kidney, pancreas, prostate, spleen, small intestine and placenta, in particular in the syncytiotrophoblast.
- O14745 NHRF1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSADAAAGAPLPRLCCLEKGPNGYGFHLHGEKGKLGQYIRLVEPGSPAEKAGLLAGDRLVEVNGENVEKETHQQVVSRIRAALNAVRLLVVDPETDEQLQKLGVQVREELLRAQEAPGQAEPPAAAEVQGAGNENEPREADKSHPEQRELRPRLCTMKKGPSGYGFNLHSDKSKPGQFIRSVDPDSPAEASGLRAQDRIVEVNGVCMEGKQHGDVVSAIRAGGDETKLLVVDRETDEFFKKCRVIPSQEHLNGPLPVPFTNGEIQKENSREALAEAALESPRPALVRSASSDTSEELNSQDSPPKQDSTAPSSTSSSDPILDFNISLAMAKERAHQKRSSKRAPQMDWSKKNELFSNL
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O14745 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | Uniprot | |
| C15 | S-Nitrosylation | Uniprot | |
| C16 | S-Nitrosylation | Uniprot | |
| K19 | Acetylation | Uniprot | |
| K34 | Acetylation | Uniprot | |
| Y38 | Phosphorylation | Uniprot | |
| S46 | Phosphorylation | Uniprot | |
| K50 | Ubiquitination | Uniprot | |
| R58 | Methylation | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| S77 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| T95 | Phosphorylation | Uniprot | |
| K101 | Ubiquitination | Uniprot | |
| T156 | Phosphorylation | P31749 (AKT1) | Uniprot |
| S162 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| Y164 | Phosphorylation | Uniprot | |
| S170 | Phosphorylation | Uniprot | |
| K172 | Ubiquitination | Uniprot | |
| S173 | Phosphorylation | Uniprot | |
| S181 | Phosphorylation | Uniprot | |
| S186 | Phosphorylation | Uniprot | |
| S191 | Phosphorylation | Uniprot | |
| C206 | S-Nitrosylation | Uniprot | |
| S217 | Phosphorylation | Uniprot | |
| K227 | Ubiquitination | Uniprot | |
| S247 | Phosphorylation | Uniprot | |
| T260 | Phosphorylation | Uniprot | |
| S269 | Phosphorylation | Uniprot | |
| S280 | Phosphorylation | P06493 (CDK1) | Uniprot |
| S288 | Phosphorylation | Uniprot | |
| S290 | Phosphorylation | P43250 (GRK6) | Uniprot |
| S291 | Phosphorylation | Uniprot | |
| T293 | Phosphorylation | Uniprot | |
| S294 | Phosphorylation | Uniprot | |
| S299 | Phosphorylation | Uniprot | |
| S302 | Phosphorylation | P06493 (CDK1) | Uniprot |
| S313 | Phosphorylation | Uniprot | |
| S316 | Phosphorylation | Uniprot | |
| S317 | Phosphorylation | Uniprot | |
| S326 | Phosphorylation | Uniprot | |
| S339 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| S340 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| S349 | Phosphorylation | Uniprot | |
| K350 | Ubiquitination | Uniprot | |
| K351 | Ubiquitination | Uniprot |
Research Backgrounds
Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli (By similarity). Involved in the regulation of phosphate reabsorption in the renal proximal tubules. Involved in sperm capacitation. May participate in the regulation of the chloride and bicarbonate homeostasis in spermatozoa.
Phosphorylated on serine residues.
Cytoplasm. Apical cell membrane. Endomembrane system>Peripheral membrane protein. Cell projection>Filopodium. Cell projection>Ruffle. Cell projection>Microvillus.
Note: Translocates from the cytoplasm to the apical cell membrane in a PODXL-dependent manner. Colocalizes with CFTR at the midpiece of sperm tail (By similarity). Colocalizes with actin in microvilli-rich apical regions of the syncytiotrophoblast. Found in microvilli, ruffling membrane and filopodia of HeLa cells. Present in lipid rafts of T-cells.
Detected in liver, kidney, pancreas, prostate, spleen, small intestine and placenta, in particular in the syncytiotrophoblast.
Homodimer, and heterodimer with SLC9A3R2. Binds the N-termini of EZR, RDX and MSN. Binds the C-termini of PDGFRA, PDGFRB, ADRB2, NOS2 and CFTR. Binds ARHGAP17, EPI64, RACK1, OPRK1, GNAQ, CTNNB1 and PLCB3. Binds PDZK1 (By similarity). Interacts with CLCN3. Binds the C-terminus of PAG1. In resting T-cells, part of a PAG1-SLC9A3R1-MSN complex which is disrupted upon TCR activation. Forms a complex with CFTR and SLC4A7. Forms a complex with SLC4A7 and ATP6V1B1. Interacts with TRPC4 (via the PDZ-binding domain). Directly interacts with HTR4 (By similarity). Interacts (via the PDZ 1 domain) with PODXL (via the C-terminal PDZ-binding motif DTHL); interaction is not detected in glomerular epithelium cells. Interacts (via the PDZ 1 domain) with PODXL (via the C-terminal PDZ-binding motif DTHL); the interaction take place early in the secretory pathway and is necessary for its apical membrane sorting (By similarity). Interacts with SLC26A3 (By similarity). Interacts with MCC. Interacts with SLC34A1. Interacts (via the PDZ domains) with SLC26A6 isoform 4 and isoform 5.
Research Fields
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
References
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