Proteasome 20S alpha 5 Antibody - #DF8268

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Product: Proteasome 20S alpha 5 Antibody
Catalog: DF8268
Description: Rabbit polyclonal antibody to Proteasome 20S alpha 5
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Chicken, Xenopus
Mol.Wt.: 26 kDa; 26kD(Calculated).
Uniprot: P28066
RRID: AB_2841557

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Proteasome 20S alpha 5 Antibody detects endogenous levels of total Proteasome 20S alpha 5.
RRID:
AB_2841557
Cite Format: Affinity Biosciences Cat# DF8268, RRID:AB_2841557.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Macropain zeta chain; Multicatalytic endopeptidase complex zeta chain; Proteasome (prosome macropain) subunit alpha type 5; Proteasome alpha5 subunit; Proteasome component 5; Proteasome subunit alpha type 5; Proteasome subunit alpha type-5; Proteasome subunit zeta; Proteasome zeta chain; PSA5_HUMAN; PSC5; PSMA5; ZETA;

Immunogens

Immunogen:
Uniprot:

P28066(PSA5_HUMAN) >>Visit HPA database.

Gene(ID):
PSMA5(5686)
Expression:
P28066 PSA5_HUMAN:

Expressed in fetal brain (at protein level).

Sequence:
MFLTRSEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGIQTSEGVCLAVEKRITSPLMEPSSIEKIVEIDAHIGCAMSGLIADAKTLIDKARVETQNHWFTYNETMTVESVTQAVSNLALQFGEEDADPGAMSRPFGVALLFGGVDEKGPQLFHMDPSGTFVQCDARAIGSASEGAQSSLQEVYHKSMTLKEAIKSSLIILKQVMEEKLNATNIELATVQPGQNFHMFTKEELEEVIKDI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Xenopus
100
Zebrafish
100
Chicken
100
Dog
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P28066 As Substrate

Site PTM Type Enzyme
S6 Phosphorylation
Y8 Phosphorylation
R10 Methylation
T14 Phosphorylation
S16 Phosphorylation
R20 Methylation
Y26 Phosphorylation
T36 Phosphorylation
S43 Phosphorylation
K52 Ubiquitination
T55 Phosphorylation
S56 Phosphorylation
S62 Phosphorylation
S63 Phosphorylation
K66 Ubiquitination
S79 Phosphorylation
K86 Ubiquitination
K91 Acetylation
K91 Ubiquitination
K149 Sumoylation
K149 Ubiquitination
S172 Phosphorylation
S174 Phosphorylation
Y185 Phosphorylation
K187 Ubiquitination
K192 Sumoylation
K192 Ubiquitination
K196 Sumoylation
K196 Ubiquitination
K203 Sumoylation
K203 Ubiquitination
K209 Acetylation
K209 Ubiquitination
T213 Phosphorylation
T219 Phosphorylation
K231 Ubiquitination
K239 Ubiquitination

Research Backgrounds

Function:

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).

Subcellular Location:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in fetal brain (at protein level).

Subunit Structure:

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is a barrel-shaped complex made of 28 subunits that are arranged in four stacked rings. The two outer rings are each formed by seven alpha subunits, and the two inner rings are formed by seven beta subunits. The proteolytic activity is exerted by three beta-subunits PSMB5, PSMB6 and PSMB7. PSMA5 interacts directly with the PSMG1-PSMG2 heterodimer which promotes 20S proteasome assembly.

Family&Domains:

Belongs to the peptidase T1A family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Proteasome.

References

1). L-leucine dietary supplementation modulates muscle protein degradation and increases pro-inflammatory cytokines in tumour-bearing rats. CYTOKINE (PubMed: 28494385) [IF=3.8]

Application: WB    Species: rat    Sample: gastrocnemius muscle tissue

Fig. 3.| Muscle mass-to-body weight ratio (%; A), total muscle protein concentrations (µg/µL; B) and proteasome subunits: 11S (% of arbitrary unity; C), 19S (% of arbitrary unity; D), and 20Sα (subunits 29 kDa and 32 kDa; (% of arbitrary unity; E)) in the gastrocnemius muscle tissue of Wistar rats with or without tumour and leucine nutritional supplementation. Data are plotted as the mean ± SD. Legend: C: Rats fed a normal protein diet; L: Rats fed a leucine-rich diet; W:Rats fed a normal protein diet and inoculated with Walker 256 tumour cells; WL:Rats fed a leucine-rich diet and inoculated with Walker 256 tumour cells. Samples were collected on the 7th, 14th and 21st days after the beginning of the experiment. **Significant differences as a result of tumour effects, two-way ANOVA with Bonferroni testing, n = 6 per group; *Significant difference as a result of leucine modulation of tumour effects, two-way ANOVA with Bonferroni testing, n = 6 per group. P ≤ 0.05.

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