KIF3A Antibody - #DF8266

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Product: KIF3A Antibody
Catalog: DF8266
Description: Rabbit polyclonal antibody to KIF3A
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 85 kDa; 80kD(Calculated).
Uniprot: Q9Y496
RRID: AB_2841555

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(92%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(92%), Chicken(92%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
KIF3A Antibody detects endogenous levels of total KIF3A.
RRID:
AB_2841555
Cite Format: Affinity Biosciences Cat# DF8266, RRID:AB_2841555.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

KIF 3; KIF 3A; KIF3; Kif3a; KIF3A_HUMAN; Kifl; Kinesin family member 3A; Kinesin family protein 3; Kinesin family protein 3A; Kinesin like protein KIF 3A; Kinesin like protein KIF3A; Kinesin-like protein KIF3A; Kns3; Microtubule plus end directed kinesin motor 3A; Microtubule plus end-directed kinesin motor 3A;

Immunogens

Immunogen:
Uniprot:

Q9Y496(KIF3A_HUMAN) >>Visit HPA database.

Gene(ID):
KIF3A(11127)
Sequence:
MPINKSEKPESCDNVKVVVRCRPLNEREKSMCYKQAVSVDEMRGTITVHKTDSSNEPPKTFTFDTVFGPESKQLDVYNLTARPIIDSVLEGYNGTIFAYGQTGTGKTFTMEGVRAIPELRGIIPNSFAHIFGHIAKAEGDTRFLVRVSYLEIYNEEVRDLLGKDQTQRLEVKERPDVGVYIKDLSAYVVNNADDMDRIMTLGHKNRSVGATNMNEHSSRSHAIFTITIECSEKGIDGNMHVRMGKLHLVDLAGSERQAKTGATGQRLKEATKINLSLSTLGNVISALVDGKSTHVPYRNSKLTRLLQDSLGGNSKTMMCANIGPADYNYDETISTLRYANRAKNIKNKARINEDPKDALLRQFQKEIEELKKKLEEGEEISGSDISGSEEDDDEEGEVGEDGEKRKKRRGKKKVSPDKMIEMQAKIDEERKALETKLDMEEEERNKARAELEKREKDLLKAQQEHQSLLEKLSALEKKVIVGGVDLLAKAEEQEKLLEESNMELEERRKRAEQLRRELEEKEQERLDIEEKYTSLQEEAQGKTKKLKKVWTMLMAAKSEMADLQQEHQREIEGLLENIRQLSRELRLQMLIIDNFIPRDYQEMIENYVHWNEDIGEWQLKCVAYTGNNMRKQTPVPDKKEKDPFEVDLSHVYLAYTEESLRQSLMKLERPRTSKGKARPKTGRRKRSAKPETVIDSLLQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Xenopus
100
Rabbit
100
Dog
92
Zebrafish
92
Chicken
92
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y496 As Substrate

Site PTM Type Enzyme
T45 Phosphorylation
T47 Phosphorylation
T51 Phosphorylation
K163 Ubiquitination
S218 Phosphorylation
T316 Phosphorylation
Y327 Phosphorylation
Y329 Phosphorylation
Y338 Phosphorylation
K346 Acetylation
K348 Acetylation
K356 Acetylation
K356 Ubiquitination
K365 Acetylation
S381 Phosphorylation
S383 Phosphorylation
S386 Phosphorylation
S388 Phosphorylation
S415 Phosphorylation
K471 Ubiquitination
K477 Acetylation
K478 Acetylation
K478 Ubiquitination
K495 Acetylation
K531 Ubiquitination
K542 Ubiquitination
Y624 Phosphorylation
T633 Phosphorylation
K666 Ubiquitination
S687 Phosphorylation Q9UQM7 (CAMK2A)
K689 Ubiquitination
T692 Phosphorylation

Research Backgrounds

Function:

Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro. Plays a role in primary cilia formation. Plays a role in centriole cohesion and subdistal appendage organization and function. Regulates the formation of the subdistal appendage via recruitment of DCTN1 to the centriole. Also required for ciliary basal feet formation and microtubule anchoring to mother centriole.

Subcellular Location:

Cytoplasm>Cytoskeleton. Cell projection>Cilium. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriole.
Note: Localizes to the subdistal appendage region of the centriole.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Heterodimer of KIF3A and KIF3B (By similarity). Interacts with PIFO. Interacts with CLN3. Interacts with DCTN1 (By similarity).

Family&Domains:

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin II subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > Hedgehog signaling pathway.   (View pathway)

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