UIMC1 Antibody | Affinity Biosciences

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Western blot analysis UIMC1 using COLO205 whole cell lysates.

DF8264 at 1/100 staining Mouse testis tissue by IHC-P.

Product:	UIMC1 Antibody
Catalog:	DF8264
Description:	Rabbit polyclonal antibody to UIMC1
Application:	WB IHC
Reactivity:	Human
Prediction:	Horse, Rabbit, Dog
Mol.Wt.:	80 kDa; 80kD(Calculated).
Uniprot:	Q96RL1
RRID:	AB_2841553

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Horse(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

UIMC1 Antibody detects endogenous levels of total UIMC1.

RRID:

AB_2841553
Cite Format: Affinity Biosciences Cat# DF8264, RRID:AB_2841553.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

BRCA1-A complex subunit RAP80; Nuclear zinc finger protein RAP80; OTTHUMP00000161441; OTTHUMP00000223372; OTTHUMP00000223374; RAP80; Receptor associated protein 80; Receptor-associated protein 80; Retinoid X receptor interacting protein 110; Retinoid x receptor interacting protein; Retinoid X receptor-interacting protein 110; RIP110; Rxrip110; Ubiquitin interaction motif containing 1; Ubiquitin interaction motif containing protein 1; Ubiquitin interaction motif-containing protein 1; UIMC1; UIMC1_HUMAN; X2HRIP110;

Immunogens

Immunogen:

Uniprot:

Q96RL1(UIMC1_HUMAN) >>Visit HPA database.

Gene(ID):

UIMC1(51720)

Expression:

Q96RL1 UIMC1_HUMAN:

Expressed in testis, ovary, thymus and heart. Expressed in germ cells of the testis.

Sequence:

Q96RL1 UIMC1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPRRKKKVKEVSESRNLEKKDVETTSSVSVKRKRRLEDAFIVISDSDGEEPKEENGLQKTKTKQSNRAKCLAKRKIAQMTEEEQFALALKMSEQEAREVNSQEEEEEELLRKAIAESLNSCRPSDASATRSRPLATGPSSQSHQEKTTDSGLTEGIWQLVPPSLFKGSHISQGNEAEEREEPWDHTEKTEEEPVSGSSGSWDQSSQPVFENVNVKSFDRCTGHSAEHTQCGKPQESTGRGSAFLKAVQGSGDTSRHCLPTLADAKGLQDTGGTVNYFWGIPFCPDGVDPNQYTKVILCQLEVYQKSLKMAQRQLLNKKGFGEPVLPRPPSLIQNECGQGEQASEKNECISEDMGDEDKEERQESRASDWHSKTKDFQESSIKSLKEKLLLEEEPTTSHGQSSQGIVEETSEEGNSVPASQSVAALTSKRSLVLMPESSAEEITVCPETQLSSSETFDLEREVSPGSRDILDGVRIIMADKEVGNKEDAEKEVAISTFSSSNQVSCPLCDQCFPPTKIERHAMYCNGLMEEDTVLTRRQKEAKTKSDSGTAAQTSLDIDKNEKCYLCKSLVPFREYQCHVDSCLQLAKADQGDGPEGSGRACSTVEGKWQQRLKNPKEKGHSEGRLLSFLEQSEHKTSDADIKSSETGAFRVPSPGMEEAGCSREMQSSFTRRDLNESPVKSFVSISEATDCLVDFKKQVTVQPGSRTRTKAGRGRRRKF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Dog

100

Rabbit

100

Pig

Bovine

Sheep

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96RL1 As Substrate

Q96RL1

Site	PTM Type	Enzyme	Source
K9	Sumoylation		Uniprot
K9	Ubiquitination		Uniprot
S12	Phosphorylation		Uniprot
K19	Ubiquitination		Uniprot
K20	Sumoylation		Uniprot
K20	Ubiquitination		Uniprot
T24	Phosphorylation		Uniprot
S26	Phosphorylation		Uniprot
S27	Phosphorylation		Uniprot
S29	Phosphorylation		Uniprot
K31	Sumoylation		Uniprot
K31	Ubiquitination		Uniprot
S44	Phosphorylation	P68400 (CSNK2A1)	Uniprot
S46	Phosphorylation	P68400 (CSNK2A1)	Uniprot
K59	Ubiquitination		Uniprot
K75	Sumoylation		Uniprot
K75	Ubiquitination		Uniprot
K90	Sumoylation		Uniprot
K90	Ubiquitination		Uniprot
R97	Methylation		Uniprot
S101	Phosphorylation		Uniprot
K112	Ubiquitination		Uniprot
T136	Phosphorylation		Uniprot
S139	Phosphorylation		Uniprot
S140	Phosphorylation		Uniprot
S142	Phosphorylation		Uniprot
K146	Ubiquitination		Uniprot
T147	Phosphorylation		Uniprot
K166	Sumoylation		Uniprot
K166	Ubiquitination		Uniprot
S171	Phosphorylation		Uniprot
K188	Ubiquitination		Uniprot
S195	Phosphorylation		Uniprot
S197	Phosphorylation		Uniprot
S198	Phosphorylation		Uniprot
S200	Phosphorylation		Uniprot
S205	Phosphorylation	Q13315 (ATM) , Q13535 (ATR)	Uniprot
K215	Ubiquitination		Uniprot
S224	Phosphorylation		Uniprot
K232	Ubiquitination		Uniprot
R239	Methylation		Uniprot
S241	Phosphorylation		Uniprot
K245	Methylation		Uniprot
K245	Sumoylation		Uniprot
K245	Ubiquitination		Uniprot
K265	Ubiquitination		Uniprot
K305	Ubiquitination		Uniprot
K308	Sumoylation		Uniprot
K308	Ubiquitination		Uniprot
K318	Ubiquitination		Uniprot
S330	Phosphorylation		Uniprot
S350	Phosphorylation		Uniprot
K358	Ubiquitination		Uniprot
K372	Ubiquitination		Uniprot
K374	Ubiquitination		Uniprot
S380	Phosphorylation		Uniprot
K382	Sumoylation		Uniprot
K382	Ubiquitination		Uniprot
S383	Phosphorylation		Uniprot
K387	Ubiquitination		Uniprot
T395	Phosphorylation		Uniprot
T396	Phosphorylation		Uniprot
S402	Phosphorylation	Q13315 (ATM)	Uniprot
S415	Phosphorylation		Uniprot
S419	Phosphorylation		Uniprot
S421	Phosphorylation		Uniprot
K428	Ubiquitination		Uniprot
S451	Phosphorylation		Uniprot
S463	Phosphorylation		Uniprot
S466	Phosphorylation		Uniprot
K480	Sumoylation		Uniprot
K480	Ubiquitination		Uniprot
K485	Sumoylation		Uniprot
K485	Ubiquitination		Uniprot
S498	Phosphorylation		Uniprot
K544	Sumoylation		Uniprot
K544	Ubiquitination		Uniprot
S545	Phosphorylation		Uniprot
S547	Phosphorylation		Uniprot
S554	Phosphorylation		Uniprot
K559	Sumoylation		Uniprot
K559	Ubiquitination		Uniprot
K562	Sumoylation		Uniprot
K562	Ubiquitination		Uniprot
K567	Ubiquitination		Uniprot
K587	Sumoylation		Uniprot
K587	Ubiquitination		Uniprot
S597	Phosphorylation		Uniprot
S602	Phosphorylation		Uniprot
K607	Sumoylation		Uniprot
K607	Ubiquitination		Uniprot
K618	Ubiquitination		Uniprot
S627	Phosphorylation	O14757 (CHEK1)	Uniprot
S632	Phosphorylation		Uniprot
K635	Sumoylation		Uniprot
K635	Ubiquitination		Uniprot
K642	Ubiquitination		Uniprot
S653	Phosphorylation		Uniprot
S662	Phosphorylation		Uniprot
S668	Phosphorylation		Uniprot
S677	Phosphorylation	P06493 (CDK1)	Uniprot
K680	Acetylation		Uniprot
S681	Phosphorylation		Uniprot
K696	Acetylation		Uniprot
K696	Ubiquitination		Uniprot
K697	Ubiquitination		Uniprot
T700	Phosphorylation		Uniprot
S705	Phosphorylation		Uniprot

Research Backgrounds

Q96RL1_UIMC1_HUMAN

Function:

Ubiquitin-binding protein. Specifically recognizes and binds 'Lys-63'-linked ubiquitin Ref.37). Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1.

PTMs:

Sumoylated.

Phosphorylated upon DNA damage by ATM or ATR.

Subcellular Location:

Nucleus.
Note: Localizes at sites of DNA damage at double-strand breaks (DSBs).

Tissue Specificity:

Expressed in testis, ovary, thymus and heart. Expressed in germ cells of the testis.

Subunit Structure:

Interacts with TSP57 (By similarity). Component of the ARISC complex, at least composed of UIMC1/RAP80, ABRAXAS1, BRCC3/BRCC36, BABAM2 and BABAM1/NBA1. Component of the BRCA1-A complex, at least composed of the BRCA1, BARD1, UIMC1/RAP80, ABRAXAS1, BRCC3/BRCC36, BABAM2 and BABAM1/NBA1. In the BRCA1-A complex, interacts directly with ABRAXAS1. Interacts with ESR1, NR6A1 and UBE2I.

Family&Domains:

The tandem UIM domains form a continuous 60 Angstrom-long alpha-helix and mediate binding to 'Lys-63'-linked ubiquitins. UIM1 and UIM2 bind to the proximal and distal ubiquitin moieties and recognize an 'Ile-44'-centered hydrophobic patch. Since UIMs don't interact with the 'Lys-63' isopeptide bond the UIM-linker region between the 2 UIM domains determines the selectivity for 'Lys-63'-linkage, and its length is very important for specificity.

The Abraxas-interacting region (AIR) mediates the interaction with ABRAXAS1.

Belongs to the RAP80 family.

Research Fields

· Genetic Information Processing > Replication and repair > Homologous recombination.

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UIMC1 Antibody - #DF8264