PNP Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from A2780 cells(LPS 4h treatment), using PNP Antibody.

Product:	PNP Antibody
Catalog:	DF8260
Description:	Rabbit polyclonal antibody to PNP
Application:	WB IHC IF/ICC
Reactivity:	Human
Prediction:	Pig, Dog
Mol.Wt.:	32 kDa; 32kD(Calculated).
Uniprot:	P00491
RRID:	AB_2841550

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(88%), Dog(88%)

Clonality:

Polyclonal

Specificity:

PNP Antibody detects endogenous levels of total PNP.

RRID:

AB_2841550
Cite Format: Affinity Biosciences Cat# DF8260, RRID:AB_2841550.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

FLJ94043; FLJ97288; FLJ97312; Inosine phosphorylase; Inosine-guanosine phosphorylase; MGC117396; MGC125915; MGC125916; NP; Np1; Nucleoside phosphorylase; PNP; Pnp1; PNPH_HUMAN; PRO1837; PUNP; Purine nucleoside orthophosphate ribosyltransferase; Purine nucleoside phosphorylase 5a; Purine nucleoside phosphorylase;

Immunogens

Immunogen:

Uniprot:

P00491(PNPH_HUMAN) >>Visit HPA database.

Gene(ID):

PNP(4860)

Expression:

P00491 PNPH_HUMAN:

Expressed in red blood cells; overexpressed in red blood cells (cytoplasm) of patients with hereditary non-spherocytic hemolytic anemia of unknown etiology.

Sequence:

P00491 PNPH_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MENGYTYEDYKNTAEWLLSHTKHRPQVAIICGSGLGGLTDKLTQAQIFDYGEIPNFPRSTVPGHAGRLVFGFLNGRACVMMQGRFHMYEGYPLWKVTFPVRVFHLLGVDTLVVTNAAGGLNPKFEVGDIMLIRDHINLPGFSGQNPLRGPNDERFGDRFPAMSDAYDRTMRQRALSTWKQMGEQRELQEGTYVMVAGPSFETVAECRVLQKLGADAVGMSTVPEVIVARHCGLRVFGFSLITNKVIMDYESLEKANHEEVLAAGKQAAQKLEQFVSILMASIPLPDKAS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Dog

Bovine

Rabbit

Horse

Sheep

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P00491 As Substrate

P00491

Site	PTM Type	Source
M1	Acetylation	Uniprot
K11	Ubiquitination	Uniprot
S19	Phosphorylation	Uniprot
K22	Ubiquitination	Uniprot
S33	Phosphorylation	Uniprot
T39	Phosphorylation	Uniprot
K95	Acetylation	Uniprot
K95	Ubiquitination	Uniprot
R168	Methylation	Uniprot
T169	Phosphorylation	Uniprot
S176	Phosphorylation	Uniprot
T177	Phosphorylation	Uniprot
K179	Ubiquitination	Uniprot
K211	Acetylation	Uniprot
K211	Ubiquitination	Uniprot
T242	Phosphorylation	Uniprot
Y249	Phosphorylation	Uniprot
S251	Phosphorylation	Uniprot
K254	Acetylation	Uniprot
K254	Ubiquitination	Uniprot
K265	Ubiquitination	Uniprot
K287	Ubiquitination	Uniprot
S289	Phosphorylation	Uniprot

Research Backgrounds

P00491_PNPH_HUMAN

Function:

The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate.

Subcellular Location:

Cytoplasm>Cytoskeleton. Cytoplasm.

Tissue Specificity:

Expressed in red blood cells; overexpressed in red blood cells (cytoplasm) of patients with hereditary non-spherocytic hemolytic anemia of unknown etiology.

Subunit Structure:

Homotrimer.

Family&Domains:

Belongs to the PNP/MTAP phosphorylase family.

Research Fields

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

· Metabolism > Metabolism of cofactors and vitamins > Nicotinate and nicotinamide metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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PNP Antibody - #DF8260