OS9 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Product:	OS9 Antibody
Catalog:	DF8249
Description:	Rabbit polyclonal antibody to OS9
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.:	76 kDa; 76kD(Calculated).
Uniprot:	Q13438
RRID:	AB_2841543

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Zebrafish(82%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(82%)

Clonality:

Polyclonal

Specificity:

OS9 Antibody detects endogenous levels of total OS9.

RRID:

AB_2841543
Cite Format: Affinity Biosciences Cat# DF8249, RRID:AB_2841543.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Amplified in osteosarcoma 9; amplified in osteosarcoma; ERLEC2; OS-9; Os9; OS9_HUMAN; Osteosarcoma amplified 9 endoplasmic reticulum lectin; Protein OS-9;

Immunogens

Immunogen:

Uniprot:

Q13438(OS9_HUMAN) >>Visit HPA database.

Gene(ID):

OS9(10956)

Expression:

Q13438 OS9_HUMAN:

Ubiquitously expressed. Found as well in all tumor cell lines analyzed, amplified in sarcomas. Highly expressed in osteosarcoma SJSA-1 and rhabdomyosarcoma Rh30 cell lines. Isoform 2 is the major isoform detected in all cell types examined.

Sequence:

Q13438 OS9_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAETLLSSLLGLLLLGLLLPASLTGGVGSLNLEELSEMRYGIEILPLPVMGGQSQSSDVVIVSSKYKQRYECRLPAGAIHFQREREEETPAYQGPGIPELLSPMRDAPCLLKTKDWWTYEFCYGRHIQQYHMEDSEIKGEVLYLGYYQSAFDWDDETAKASKQHRLKRYHSQTYGNGSKCDLNGRPREAEVRFLCDEGAGISGDYIDRVDEPLSCSYVLTIRTPRLCPHPLLRPPPSAAPQAILCHPSLQPEEYMAYVQRQADSKQYGDKIIEELQDLGPQVWSETKSGVAPQKMAGASPTKDDSKDSDFWKMLNEPEDQAPGGEEVPAEEQDPSPEAADSASGAPNDFQNNVQVKVIRSPADLIRFIEELKGGTKKGKPNIGQEQPVDDAAEVPQREPEKERGDPERQREMEEEEDEDEDEDEDEDERQLLGEFEKELEGILLPSDRDRLRSEVKAGMERELENIIQETEKELDPDGLKKESERDRAMLALTSTLNKLIKRLEEKQSPELVKKHKKKRVVPKKPPPSPQPTEEDPEHRVRVRVTKLRLGGPNQDLTVLEMKRENPQLKQIEGLVKELLEREGLTAAGKIEIKIVRPWAEGTEEGARWLTDEDTRNLKEIFFNILVPGAEEAQKERQRQKELESNYRRVWGSPGGEGTGDLDEFDF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13438 As Substrate

Q13438

Site	PTM Type	Source
K113	Ubiquitination	Uniprot
K115	Ubiquitination	Uniprot
K180	Ubiquitination	Uniprot
K271	Ubiquitination	Uniprot
K288	Ubiquitination	Uniprot
K295	Ubiquitination	Uniprot
S300	Phosphorylation	Uniprot
K303	Ubiquitination	Uniprot
K307	Ubiquitination	Uniprot
S361	Phosphorylation	Uniprot
K373	Ubiquitination	Uniprot
K380	Ubiquitination	Uniprot
K402	Ubiquitination	Uniprot
K438	Ubiquitination	Uniprot
K457	Ubiquitination	Uniprot
K473	Ubiquitination	Uniprot
K481	Ubiquitination	Uniprot
K499	Ubiquitination	Uniprot
K507	Ubiquitination	Uniprot
S509	Phosphorylation	Uniprot
K514	Ubiquitination	Uniprot
S529	O-Glycosylation	Uniprot
S529	Phosphorylation	Uniprot
T533	O-Glycosylation	Uniprot
K563	Ubiquitination	Uniprot
K570	Ubiquitination	Uniprot
K577	Ubiquitination	Uniprot
K594	Ubiquitination	Uniprot
T603	Phosphorylation	Uniprot
T611	Phosphorylation	Uniprot
K619	Ubiquitination	Uniprot
K635	Ubiquitination	Uniprot
K641	Ubiquitination	Uniprot

Research Backgrounds

Q13438_OS9_HUMAN

Function:

Lectin which functions in endoplasmic reticulum (ER) quality control and ER-associated degradation (ERAD). May bind terminally misfolded non-glycosylated proteins as well as improperly folded glycoproteins, retain them in the ER, and possibly transfer them to the ubiquitination machinery and promote their degradation. Possible targets include TRPV4.

PTMs:

Intramolecular disulfide bonds.

Isoform 1 and isoform 2 are N-glycosylated.

Subcellular Location:

Endoplasmic reticulum lumen.

Tissue Specificity:

Subunit Structure:

Interacts (via C-terminus) with CPNE6 (via second C2 domain); this interaction occurs in a calcium-dependent manner in vitro (By similarity). Probably part of the HRD1 ubiquitin ligase complex composed at least of SYVN1/HRD1 and SEL1L with which it interacts directly. Through this complex it may interact with ERLEC1 and HSPA5. Interacts with DERL2. Interacts with HSP90B1. Interacts with CREB3. Interacts with SYVN1.

Family&Domains:

Belongs to the OS-9 family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)

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OS9 Antibody - #DF8249