EEA1 Antibody - #DF8248

(5)  
Product: EEA1 Antibody
Catalog: DF8248
Description: Rabbit polyclonal antibody to EEA1
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.: 180 kDa; 162kD(Calculated).
Uniprot: Q15075
RRID: AB_2841542

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Bovine(83%), Horse(100%), Sheep(92%), Dog(92%)
Clonality:
Polyclonal
Specificity:
EEA1 Antibody detects endogenous levels of total EEA1.
RRID:
AB_2841542
Cite Format: Affinity Biosciences Cat# DF8248, RRID:AB_2841542.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Early endosome antigen 1; Early endosome antigen 1, 162kD; Early endosome associated protein; EEA 1; EEA1; EEA1_HUMAN; Endosome associated protein p162; Endosome-associated protein p162; MST105; MSTP105; ZFYVE2; Zinc finger FYVE domain containing protein 2; Zinc finger FYVE domain-containing protein 2;

Immunogens

Immunogen:
Uniprot:

Q15075(EEA1_HUMAN) >>Visit HPA database.

Gene(ID):
EEA1(8411)
Sequence:
MLRRILQRTPGRVGSQGSDLDSSATPINTVDVNNESSSEGFICPQCMKSLGSADELFKHYEAVHDAGNDSGHGGESNLALKRDDVTLLRQEVQDLQASLKEEKWYSEELKKELEKYQGLQQQEAKPDGLVTDSSAELQSLEQQLEEAQTENFNIKQMKDLFEQKAAQLATEIADIKSKYDEERSLREAAEQKVTRLTEELNKEATVIQDLKTELLQRPGIEDVAVLKKELVQVQTLMDNMTLERERESEKLKDECKKLQSQYASSEATISQLRSELAKGPQEVAVYVQELQKLKSSVNELTQKNQTLTENLLKKEQDYTKLEEKHNEESVSKKNIQATLHQKDLDCQQLQSRLSASETSLHRIHVELSEKGEATQKLKEELSEVETKYQHLKAEFKQLQQQREEKEQHGLQLQSEINQLHSKLLETERQLGEAHGRLKEQRQLSSEKLMDKEQQVADLQLKLSRLEEQLKEKVTNSTELQHQLDKTKQQHQEQQALQQSTTAKLREAQNDLEQVLRQIGDKDQKIQNLEALLQKSKENISLLEKEREDLYAKIQAGEGETAVLNQLQEKNHTLQEQVTQLTEKLKNQSESHKQAQENLHDQVQEQKAHLRAAQDRVLSLETSVNELNSQLNESKEKVSQLDIQIKAKTELLLSAEAAKTAQRADLQNHLDTAQNALQDKQQELNKITTQLDQVTAKLQDKQEHCSQLESHLKEYKEKYLSLEQKTEELEGQIKKLEADSLEVKASKEQALQDLQQQRQLNTDLELRATELSKQLEMEKEIVSSTRLDLQKKSEALESIKQKLTKQEEEKKILKQDFETLSQETKIQHEELNNRIQTTVTELQKVKMEKEALMTELSTVKDKLSKVSDSLKNSKSEFEKENQKGKAAILDLEKTCKELKHQLQVQMENTLKEQKELKKSLEKEKEASHQLKLELNSMQEQLIQAQNTLKQNEKEEQQLQGNINELKQSSEQKKKQIEALQGELKIAVLQKTELENKLQQQLTQAAQELAAEKEKISVLQNNYEKSQETFKQLQSDFYGRESELLATRQDLKSVEEKLSLAQEDLISNRNQIGNQNKLIQELKTAKATLEQDSAKKEQQLQERCKALQDIQKEKSLKEKELVNEKSKLAEIEEIKCRQEKEITKLNEELKSHKLESIKEITNLKDAKQLLIQQKLELQGKADSLKAAVEQEKRNQQILKDQVKKEEEELKKEFIEKEAKLHSEIKEKEVGMKKHEENEAKLTMQITALNENLGTVKKEWQSSQRRVSELEKQTDDLRGEIAVLEATVQNNQDERRALLERCLKGEGEIEKLQTKVLELQRKLDNTTAAVQELGRENQSLQIKHTQALNRKWAEDNEVQNCMACGKGFSVTVRRHHCRQCGNIFCAECSAKNALTPSSKKPVRVCDACFNDLQG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
92
Dog
92
Bovine
83
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15075 As Substrate

Site PTM Type Enzyme
T9 Phosphorylation
S15 Phosphorylation
S18 Phosphorylation
S22 Phosphorylation
S23 Phosphorylation
T25 Phosphorylation
S49 Phosphorylation
S52 Phosphorylation
K58 Ubiquitination
S70 Phosphorylation
K81 Ubiquitination
S98 Phosphorylation
K100 Ubiquitination
S139 Phosphorylation
K158 Acetylation
K164 Acetylation
K164 Ubiquitination
K202 Ubiquitination
K228 Ubiquitination
T235 Phosphorylation
T241 Phosphorylation
S248 Phosphorylation
Y286 Phosphorylation
S295 Phosphorylation
S296 Phosphorylation
T301 Phosphorylation
S354 Phosphorylation
S356 Phosphorylation
S382 Phosphorylation
K422 Ubiquitination
K544 Ubiquitination
K552 Ubiquitination
T560 Phosphorylation
K592 Ubiquitination
S618 Phosphorylation
T621 Phosphorylation
S628 Phosphorylation
K634 Acetylation
S638 Phosphorylation
K645 Acetylation
T761 Phosphorylation
K824 Ubiquitination
T836 Phosphorylation
T837 Phosphorylation
K848 Methylation
T853 Phosphorylation
S856 Phosphorylation
T857 Phosphorylation
S863 Phosphorylation
K884 Ubiquitination
K895 Ubiquitination
T908 Phosphorylation
S926 Phosphorylation
K995 Ubiquitination
S1015 Phosphorylation
Y1021 Phosphorylation
S1024 Phosphorylation
T1027 Phosphorylation
K1029 Ubiquitination
K1125 Ubiquitination
K1133 Acetylation
K1138 Acetylation
K1142 Acetylation
T1159 Phosphorylation
K1178 Ubiquitination
K1183 Ubiquitination
T1252 Phosphorylation
S1265 Phosphorylation
K1312 Ubiquitination
K1319 Ubiquitination
K1340 Ubiquitination
T1392 Phosphorylation Q16539 (MAPK14)

Research Backgrounds

Function:

Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.

Subcellular Location:

Cytoplasm. Early endosome membrane>Peripheral membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer. Binds STX6. Binds RAB5A, RAB5B, RAB5C and RAB22A that have been activated by GTP-binding. Interacts with RAB31. Interacts with ERBB2. Interacts with SAMD9 AND SAMD9L. May interact with PLEKHF2.

Family&Domains:

The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

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