CTSS Antibody | Affinity Biosciences

WB
Cites

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Western blot analysis of extracts from HepG2 cells(heat-shock treatment), using CTSS Antibody.

Figure 7 Validation of four candidates by Western blot and the correlation analysis between four candidates and behavior tests.

Product:	CTSS Antibody
Catalog:	DF8246
Description:	Rabbit polyclonal antibody to CTSS
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	37 kDa; 37kD(Calculated).
Uniprot:	P25774
RRID:	AB_2841541

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(83%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(92%), Dog(83%)

Clonality:

Polyclonal

Specificity:

CTSS Antibody detects endogenous levels of total CTSS.

RRID:

AB_2841541
Cite Format: Affinity Biosciences Cat# DF8246, RRID:AB_2841541.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Cathepsin S; CathepsinS; CATS_HUMAN; Ctss;

Immunogens

Immunogen:

Uniprot:

P25774(CATS_HUMAN) >>Visit HPA database.

Gene(ID):

CTSS(1520)

Sequence:

P25774 CATS_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKRLVCVLLVCSSAVAQLHKDPTLDHHWHLWKKTYGKQYKEKNEEAVRRLIWEKNLKFVMLHNLEHSMGMHSYDLGMNHLGDMTSEEVMSLMSSLRVPSQWQRNITYKSNPNRILPDSVDWREKGCVTEVKYQGSCGACWAFSAVGALEAQLKLKTGKLVSLSAQNLVDCSTEKYGNKGCNGGFMTTAFQYIIDNKGIDSDASYPYKAMDQKCQYDSKYRAATCSKYTELPYGREDVLKEAVANKGPVSVGVDARHPSFFLYRSGVYYEPSCTQNVNHGVLVVGYGDLNGKEYWLVKNSWGHNFGEEGYIRMARNKGNHCGIASFPSYPEI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

Sheep

Rabbit

Pig

Dog

Xenopus

Chicken

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P25774 As Substrate

P25774

Site	PTM Type	Source
K42	Ubiquitination	Uniprot
S171	Phosphorylation	Uniprot
Y175	Phosphorylation	Uniprot
S200	Phosphorylation	Uniprot
Y215	Phosphorylation	Uniprot
S217	Phosphorylation	Uniprot
Y232	Phosphorylation	Uniprot
Y262	Phosphorylation	Uniprot

Research Backgrounds

P25774_CATS_HUMAN

Function:

Thiol protease. Key protease responsible for the removal of the invariant chain from MHC class II molecules. The bond-specificity of this proteinase is in part similar to the specificities of cathepsin L.

Subcellular Location:

Lysosome. Secreted.

Subunit Structure:

Monomer.

Family&Domains:

Belongs to the peptidase C1 family.

Research Fields

· Cellular Processes > Transport and catabolism > Lysosome. (View pathway)

· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Organismal Systems > Immune system > Antigen processing and presentation. (View pathway)

References

1). Integrated Multiomics Analysis Identifies a Novel Biomarker Associated with Prognosis in Intracerebral Hemorrhage. Oxidative Medicine and Cellular Longevity, 2021 (PubMed: 36226158)

Application: WB Species: Mouse Sample:

Figure 7 Validation of four candidates by Western blot and the correlation analysis between four candidates and behavior tests. (a) Representative Western blot bands and quantitative analysis of Itgb2, Serpina3n, Serping1, and Ctss protein expression. (b) Spearman's correlation analysis results showing the correlation between the protein expression levels of candidates and the scores of mGarcia test. (c) Spearman's correlation analysis results showing the correlation between the protein expression levels of candidates and the scores of beam walking test. n = 6 per group.

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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CTSS Antibody - #DF8246