UBE2M Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/3

Western blot analysis of UBE2M Antibody expression in 293T cells lysates.

DF8243 at 1/100 staining Mouse lung tissue by IHC-P.

Product:	UBE2M Antibody
Catalog:	DF8243
Description:	Rabbit polyclonal antibody to UBE2M
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Rabbit, Dog
Mol.Wt.:	21 kDa; 21kD(Calculated).
Uniprot:	P61081
RRID:	AB_2841538

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

UBE2M Antibody detects endogenous levels of total UBE2M.

RRID:

AB_2841538
Cite Format: Affinity Biosciences Cat# DF8243, RRID:AB_2841538.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

hUbc12; NEDD8 carrier protein; NEDD8 conjugating enzyme Ubc12; NEDD8 protein ligase; NEDD8-conjugating enzyme Ubc12; UBC-RS2; UBC12; UBC12_HUMAN; ube2m; ubiquitin carrier protein M; Ubiquitin conjugating enzyme E2 M; Ubiquitin-conjugating enzyme E2 M; ubiquitin-protein ligase M; yeast UBC12 homolog;

Immunogens

Immunogen:

Uniprot:

P61081(UBC12_HUMAN) >>Visit HPA database.

Gene(ID):

UBE2M(9040)

Sequence:

P61081 UBC12_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MIKLFSLKQQKKEEESAGGTKGSSKKASAAQLRIQKDINELNLPKTCDISFSDPDDLLNFKLVICPDEGFYKSGKFVFSFKVGQGYPHDPPKVKCETMVYHPNIDLEGNVCLNILREDWKPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLFEQNVQRSMRGGYIGSTYFERCLK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Dog

100

Rabbit

100

Xenopus

Chicken

Sheep

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P61081 As Substrate

P61081

Site	PTM Type	Source
M1	Acetylation	Uniprot
K3	Acetylation	Uniprot
K3	Ubiquitination	Uniprot
S6	Phosphorylation	Uniprot
K8	Ubiquitination	Uniprot
T20	Phosphorylation	Uniprot
S23	Phosphorylation	Uniprot
K26	Ubiquitination	Uniprot
S28	Phosphorylation	Uniprot
R33	Methylation	Uniprot
K36	Ubiquitination	Uniprot
K45	Ubiquitination	Uniprot
T46	Phosphorylation	Uniprot
C47	S-Nitrosylation	Uniprot
S50	Phosphorylation	Uniprot
K61	Methylation	Uniprot
K61	Ubiquitination	Uniprot
C65	S-Nitrosylation	Uniprot
K72	Ubiquitination	Uniprot
K75	Ubiquitination	Uniprot
K81	Ubiquitination	Uniprot
Y86	Phosphorylation	Uniprot
K92	Ubiquitination	Uniprot
K94	Ubiquitination	Uniprot
R157	Methylation	Uniprot
R166	Methylation	Uniprot
R169	Methylation	Uniprot
Y172	Phosphorylation	Uniprot
Y177	Phosphorylation	Uniprot
R180	Methylation	Uniprot
C181	S-Nitrosylation	Uniprot

Research Backgrounds

P61081_UBC12_HUMAN

Function:

Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.

PTMs:

The acetylation of Met-1 increases affinity for DCUN1D1 by about 2 orders of magnitude and is crucial for NEDD8 transfer to cullins.

Subunit Structure:

Interacts with UBA3 and RBX1. Interacts (acetylated at N-terminal methionine) with DCUN1D1 (via DCUN1 domain).

Family&Domains:

Both the N-terminal docking peptide and the catalytic core domain must bind the UBA3-NAE1 complex simultaneously for optimal transfer of NEDD8.

Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)

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UBE2M Antibody - #DF8243