ABCA1 Antibody - #DF8233

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Product: ABCA1 Antibody
Catalog: DF8233
Description: Rabbit polyclonal antibody to ABCA1
Application: WB
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 254 kDa; 254kD(Calculated).
Uniprot: O95477
RRID: AB_2841530

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
ABCA1 Antibody detects endogenous levels of total ABCA1.
RRID:
AB_2841530
Cite Format: Affinity Biosciences Cat# DF8233, RRID:AB_2841530.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ABC 1; ABC Transporter 1; ABC-1; ABC1; ABCA 1; ABCA1; ABCA1_HUMAN; ATP binding Cassette 1; ATP binding cassette sub family A ABC1 member 1; ATP binding cassette sub family A member 1; ATP binding Cassette Transporter 1; ATP-binding cassette 1; ATP-binding cassette sub-family A member 1; ATP-binding cassette transporter 1; CERP; Cholesterol efflux regulatory protein; FLJ14958; HDLDT1; Membrane bound; MGC164864; MGC165011; TD; TGD;

Immunogens

Immunogen:
Uniprot:

O95477(ABCA1_HUMAN) >>Visit HPA database.

Gene(ID):
ABCA1(19)
Expression:
O95477 ABCA1_HUMAN:

Widely expressed, but most abundant in macrophages.

Sequence:
MACWPQLRLLLWKNLTFRRRQTCQLLLEVAWPLFIFLILISVRLSYPPYEQHECHFPNKAMPSAGTLPWVQGIICNANNPCFRYPTPGEAPGVVGNFNKSIVARLFSDARRLLLYSQKDTSMKDMRKVLRTLQQIKKSSSNLKLQDFLVDNETFSGFLYHNLSLPKSTVDKMLRADVILHKVFLQGYQLHLTSLCNGSKSEEMIQLGDQEVSELCGLPREKLAAAERVLRSNMDILKPILRTLNSTSPFPSKELAEATKTLLHSLGTLAQELFSMRSWSDMRQEVMFLTNVNSSSSSTQIYQAVSRIVCGHPEGGGLKIKSLNWYEDNNYKALFGGNGTEEDAETFYDNSTTPYCNDLMKNLESSPLSRIIWKALKPLLVGKILYTPDTPATRQVMAEVNKTFQELAVFHDLEGMWEELSPKIWTFMENSQEMDLVRMLLDSRDNDHFWEQQLDGLDWTAQDIVAFLAKHPEDVQSSNGSVYTWREAFNETNQAIRTISRFMECVNLNKLEPIATEVWLINKSMELLDERKFWAGIVFTGITPGSIELPHHVKYKIRMDIDNVERTNKIKDGYWDPGPRADPFEDMRYVWGGFAYLQDVVEQAIIRVLTGTEKKTGVYMQQMPYPCYVDDIFLRVMSRSMPLFMTLAWIYSVAVIIKGIVYEKEARLKETMRIMGLDNSILWFSWFISSLIPLLVSAGLLVVILKLGNLLPYSDPSVVFVFLSVFAVVTILQCFLISTLFSRANLAAACGGIIYFTLYLPYVLCVAWQDYVGFTLKIFASLLSPVAFGFGCEYFALFEEQGIGVQWDNLFESPVEEDGFNLTTSVSMMLFDTFLYGVMTWYIEAVFPGQYGIPRPWYFPCTKSYWFGEESDEKSHPGSNQKRISEICMEEEPTHLKLGVSIQNLVKVYRDGMKVAVDGLALNFYEGQITSFLGHNGAGKTTTMSILTGLFPPTSGTAYILGKDIRSEMSTIRQNLGVCPQHNVLFDMLTVEEHIWFYARLKGLSEKHVKAEMEQMALDVGLPSSKLKSKTSQLSGGMQRKLSVALAFVGGSKVVILDEPTAGVDPYSRRGIWELLLKYRQGRTIILSTHHMDEADVLGDRIAIISHGKLCCVGSSLFLKNQLGTGYYLTLVKKDVESSLSSCRNSSSTVSYLKKEDSVSQSSSDAGLGSDHESDTLTIDVSAISNLIRKHVSEARLVEDIGHELTYVLPYEAAKEGAFVELFHEIDDRLSDLGISSYGISETTLEEIFLKVAEESGVDAETSDGTLPARRNRRAFGDKQSCLRPFTEDDAADPNDSDIDPESRETDLLSGMDGKGSYQVKGWKLTQQQFVALLWKRLLIARRSRKGFFAQIVLPAVFVCIALVFSLIVPPFGKYPSLELQPWMYNEQYTFVSNDAPEDTGTLELLNALTKDPGFGTRCMEGNPIPDTPCQAGEEEWTTAPVPQTIMDLFQNGNWTMQNPSPACQCSSDKIKKMLPVCPPGAGGLPPPQRKQNTADILQDLTGRNISDYLVKTYVQIIAKSLKNKIWVNEFRYGGFSLGVSNTQALPPSQEVNDAIKQMKKHLKLAKDSSADRFLNSLGRFMTGLDTKNNVKVWFNNKGWHAISSFLNVINNAILRANLQKGENPSHYGITAFNHPLNLTKQQLSEVALMTTSVDVLVSICVIFAMSFVPASFVVFLIQERVSKAKHLQFISGVKPVIYWLSNFVWDMCNYVVPATLVIIIFICFQQKSYVSSTNLPVLALLLLLYGWSITPLMYPASFVFKIPSTAYVVLTSVNLFIGINGSVATFVLELFTDNKLNNINDILKSVFLIFPHFCLGRGLIDMVKNQAMADALERFGENRFVSPLSWDLVGRNLFAMAVEGVVFFLITVLIQYRFFIRPRPVNAKLSPLNDEDEDVRRERQRILDGGGQNDILEIKELTKIYRRKRKPAVDRICVGIPPGECFGLLGVNGAGKSSTFKMLTGDTTVTRGDAFLNKNSILSNIHEVHQNMGYCPQFDAITELLTGREHVEFFALLRGVPEKEVGKVGEWAIRKLGLVKYGEKYAGNYSGGNKRKLSTAMALIGGPPVVFLDEPTTGMDPKARRFLWNCALSVVKEGRSVVLTSHSMEECEALCTRMAIMVNGRFRCLGSVQHLKNRFGDGYTIVVRIAGSNPDLKPVQDFFGLAFPGSVLKEKHRNMLQYQLPSSLSSLARIFSILSQSKKRLHIEDYSVSQTTLDQVFVNFAKDQSDDDHLKDLSLHKNQTVVDVAVLTSFLQDEKVKESYV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Chicken
92
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95477 As Substrate

Site PTM Type Enzyme
N98 N-Glycosylation
S116 Phosphorylation
S231 Phosphorylation
N244 N-Glycosylation
Y325 Phosphorylation
S884 Phosphorylation
S1031 Phosphorylation
S1042 Phosphorylation P17612 (PRKACA)
S1145 Phosphorylation
Y1206 Phosphorylation
T1242 Phosphorylation P68400 (CSNK2A1)
T1243 Phosphorylation P68400 (CSNK2A1)
S1255 Phosphorylation P68400 (CSNK2A1)
S1262 Phosphorylation
T1286 Phosphorylation
S1296 Phosphorylation
T1305 Phosphorylation
S1536 Phosphorylation
S1540 Phosphorylation
S1548 Phosphorylation
T1582 Phosphorylation
T1586 Phosphorylation
K2050 Acetylation
S2054 Phosphorylation P00533 (EGFR) , P17612 (PRKACA)
S2101 Phosphorylation
S2234 Phosphorylation

Research Backgrounds

Function:

Catalyzes the translocation of specific phospholipids from the cytoplasmic to the extracellular/lumenal leaflet of membrane coupled to the hydrolysis of ATP. Thereby, participates to phospholipids transfer to apoliproteins to form nascent high density lipoproteins/HDLs. Transports preferentially phosphatidylcholine over phosphatidylserine. May play a similar role in the efflux of intracellular cholesterol to apoliproteins and the formation of nascent high density lipoproteins/HDLs.

PTMs:

Phosphorylation on Ser-2054 regulates phospholipid efflux.

Palmitoylation by DHHC8 is essential for membrane localization.

Subcellular Location:

Membrane>Multi-pass membrane protein. Cell membrane. Endosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed, but most abundant in macrophages.

Subunit Structure:

Interacts with MEGF10. May interact with APOE1; functionally associated with APOE1 in the biogenesis of HDLs.

Family&Domains:

Multifunctional polypeptide with two homologous halves, each containing a hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain.

Belongs to the ABC transporter superfamily. ABCA family.

Research Fields

· Environmental Information Processing > Membrane transport > ABC transporters.

· Organismal Systems > Digestive system > Fat digestion and absorption.

· Organismal Systems > Digestive system > Cholesterol metabolism.

References

1). Yin-xing-tong-mai decoction attenuates atherosclerosis via activating PPARγ-LXRα-ABCA1/ABCG1 pathway. Pharmacological research, 2021 (PubMed: 33932607) [IF=9.3]
2). MaiJiTong granule attenuates atherosclerosis by reducing ferroptosis via activating STAT6-mediated inhibition of DMT1 and SOCS1/p53 pathways in LDLR-/- mice. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2024 (PubMed: 38569295) [IF=7.9]
3). Proteus mirabilis Vesicles Induce Mitochondrial Apoptosis by Regulating miR96-5p/Abca1 to Inhibit Osteoclastogenesis and Bone Loss. Frontiers in Immunology, 2022 (PubMed: 35242136) [IF=7.3]

Application: WB    Species: mouse    Sample:

FIGURE 6| Effect of P.M OMVs on miR96/Abca1 mediated mitochondria-dependent apoptosis.(J, K) Expression of Abca1 after 0.15 μg/ml of P.M OMV treatment by RT-qPCR and WB.
4). Fatty acid binding protein 3 deficiency limits atherosclerosis development via macrophage foam cell formation inhibition. EXPERIMENTAL CELL RESEARCH, 2021 (PubMed: 34370993) [IF=3.7]

Application: WB    Species: Mice    Sample: macrophages

Fig. 3. FABP3 knockdown reduced macrophage foam cell formation. Murine peritoneal macrophages were maintained in the special culture medium at 37 ◦ C in a 5 % CO 2 atmosphere. The cells were infected with lentivirus carrying shFABP3 or shRNA NC for 24 h and subsequently exposed with ox-LDL (50 μ g/ml) for 24 h. (A) Representative Oil Red O-stained images of macrophages and quantification of the staining. (B) Immunoblots of SRA1, CD36, SRB1, ABCA1, and ABCG1 in macrophages. (C) Quantification of relative mRNA expression of SRA1, CD36, SRB1, ABCA1, and ABCG1. **p < 0.01 denote significant differences between the Control and the ox-LDL groups, && p < 0.01 denote significant differences between the ox-LDL shRNA NC and ox-LDL + shFABP3 groups.

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