Product: GFPT1 Antibody
Catalog: DF8232
Description: Rabbit polyclonal antibody to GFPT1
Application: WB IHC
Reactivity: Human, Rat
Prediction: Pig, Bovine, Horse, Rabbit, Dog, Chicken
Mol.Wt.: 79 kDa; 79kD(Calculated).
Uniprot: Q06210
RRID: AB_2841529

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Pig(92%), Bovine(92%), Horse(100%), Rabbit(92%), Dog(92%), Chicken(92%)
Clonality:
Polyclonal
Specificity:
GFPT1 Antibody detects endogenous levels of total GFPT1.
RRID:
AB_2841529
Cite Format: Affinity Biosciences Cat# DF8232, RRID:AB_2841529.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CMS12; CMSTA1; D-fructose-6-phosphate amidotransferase 1; GFA; GFAT 1; GFAT; GFAT1; GFAT1m; GFPT; Gfpt1; GFPT1_HUMAN; GFPT1L; Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] 1; Glutamine--fructose-6-phosphate transaminase 1; Glutamine:fructose 6 phosphate amidotransferase 1; Hexosephosphate aminotransferase 1; MSLG;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q06210 GFPT1_HUMAN:

Isoform 1 is predominantly expressed in skeletal muscle. Not expressed in brain. Seems to be selectively expressed in striated muscle.

Sequence:
MCGIFAYLNYHVPRTRREILETLIKGLQRLEYRGYDSAGVGFDGGNDKDWEANACKIQLIKKKGKVKALDEEVHKQQDMDLDIEFDVHLGIAHTRWATHGEPSPVNSHPQRSDKNNEFIVIHNGIITNYKDLKKFLESKGYDFESETDTETIAKLVKYMYDNRESQDTSFTTLVERVIQQLEGAFALVFKSVHFPGQAVGTRRGSPLLIGVRSEHKLSTDHIPILYRTARTQIGSKFTRWGSQGERGKDKKGSCNLSRVDSTTCLFPVEEKAVEYYFASDASAVIEHTNRVIFLEDDDVAAVVDGRLSIHRIKRTAGDHPGRAVQTLQMELQQIMKGNFSSFMQKEIFEQPESVVNTMRGRVNFDDYTVNLGGLKDHIKEIQRCRRLILIACGTSYHAGVATRQVLEELTELPVMVELASDFLDRNTPVFRDDVCFFLSQSGETADTLMGLRYCKERGALTVGITNTVGSSISRETDCGVHINAGPEIGVASTKAYTSQFVSLVMFALMMCDDRISMQERRKEIMLGLKRLPDLIKEVLSMDDEIQKLATELYHQKSVLIMGRGYHYATCLEGALKIKEITYMHSEGILAGELKHGPLALVDKLMPVIMIIMRDHTYAKCQNALQQVVARQGRPVVICDKEDTETIKNTKRTIKVPHSVDCLQGILSVIPLQLLAFHLAVLRGYDVDFPRNLAKSVTVE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Pig
92
Bovine
92
Dog
92
Chicken
92
Rabbit
92
Zebrafish
75
Xenopus
73
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q06210 As Substrate

Site PTM Type Enzyme
C2 S-Nitrosylation
K25 Ubiquitination
K48 Ubiquitination
K56 Ubiquitination
S103 Phosphorylation
K139 Ubiquitination
T149 Phosphorylation
K154 Ubiquitination
K157 Ubiquitination
K190 Ubiquitination
T201 Phosphorylation
S205 Phosphorylation P17612 (PRKACA)
K216 Ubiquitination
S235 Phosphorylation P17612 (PRKACA)
S242 Phosphorylation Q13131 (PRKAA1)
K251 Ubiquitination
S253 Phosphorylation
S257 Phosphorylation
S261 Phosphorylation Q13131 (PRKAA1) , Q9UQM7 (CAMK2A)
T262 Phosphorylation
T263 Phosphorylation
Y275 Phosphorylation
Y276 Phosphorylation
K345 Ubiquitination
Y367 Phosphorylation
T368 Phosphorylation
K375 Ubiquitination
T467 Phosphorylation
S470 Phosphorylation
S473 Phosphorylation
C478 S-Nitrosylation
K536 Ubiquitination
K547 Acetylation
K547 Ubiquitination
Y553 Phosphorylation
S557 Phosphorylation
K640 Ubiquitination
K650 Acetylation
K694 Ubiquitination
S695 Phosphorylation

Research Backgrounds

Function:

Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins. Regulates the circadian expression of clock genes ARNTL/BMAL1 and CRY1.

Tissue Specificity:

Isoform 1 is predominantly expressed in skeletal muscle. Not expressed in brain. Seems to be selectively expressed in striated muscle.

Subunit Structure:

Homotetramer (Probable), may also exist as homodimers.

Research Fields

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Metabolism > Amino acid metabolism > Alanine, aspartate and glutamate metabolism.

· Metabolism > Carbohydrate metabolism > Amino sugar and nucleotide sugar metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

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