TSPO Antibody - #DF8227

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Product: TSPO Antibody
Catalog: DF8227
Description: Rabbit polyclonal antibody to TSPO
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Dog
Mol.Wt.: 19 kDa; 19kD(Calculated).
Uniprot: P30536
RRID: AB_2841527

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Dog(85%)
Clonality:
Polyclonal
Specificity:
TSPO Antibody detects endogenous levels of total TSPO.
RRID:
AB_2841527
Cite Format: Affinity Biosciences Cat# DF8227, RRID:AB_2841527.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Benzodiazapine receptor (peripheral); Benzodiazepine peripheral binding site; BPBS; BZRP; DBI; IBP; Isoquinoline carboxamide-binding protein; MBR; mDRC; Mitochondrial benzodiazepine receptor; PBR; PBS; Peripheral benzodiazepine receptor; Peripheral benzodiazepine receptor-related protein; Peripheral type benzodiazepine receptor; Peripheral-type benzodiazepine receptor; pk18; PKBS; PTBR; Ptbzr; PTBZR02; RATPTBZR02; translocator protein (18kDa); Translocator protein; Tspo; Tspo1; TSPOA_HUMAN;

Immunogens

Immunogen:
Uniprot:

P30536(TSPO_HUMAN) >>Visit HPA database.

Gene(ID):
TSPO(706)
Expression:
P30536 TSPO_HUMAN:

Found in many tissue types. Expressed at the highest levels under normal conditions in tissues that synthesize steroids.

Sequence:
MAPPWVPAMGFTLAPSLGCFVGSRFVHGEGLRWYAGLQKPSWHPPHWVLGPVWGTLYSAMGYGSYLVWKELGGFTEKAVVPLGLYTGQLALNWAWPPIFFGARQMGWALVDLLLVSGAAAATTVAWYQVSPLAARLLYPYLAWLAFTTTLNYCVWRDNHGWRGGRRLPE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
85
Pig
75
Horse
62
Sheep
62
Bovine
54
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P30536 As Substrate

Site PTM Type Enzyme

Research Backgrounds

Function:

Can bind protoporphyrin IX and may play a role in the transport of porphyrins and heme (By similarity). Promotes the transport of cholesterol across mitochondrial membranes and may play a role in lipid metabolism, but its precise physiological role is controversial. It is apparently not required for steroid hormone biosynthesis. Was initially identified as peripheral-type benzodiazepine receptor; can also bind isoquinoline carboxamides.

Subcellular Location:

Mitochondrion membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Found in many tissue types. Expressed at the highest levels under normal conditions in tissues that synthesize steroids.

Subunit Structure:

Interacts with TSPOAP1 (By similarity). May interact with STAR (By similarity). Interacts with MOST-1.

Family&Domains:

Belongs to the TspO/BZRP family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Organismal Systems > Digestive system > Cholesterol metabolism.

References

1). Inflammatory Immune Process and Depression-like Behavior in Hypothyroid Rats: A [18F] DPA-714 Micro Positron Emission Tomography Study. Pharmaceuticals (PubMed: 37259424) [IF=4.6]

Application: IHC    Species: Rat    Sample: hippocampus

Figure 5. Immunohistochemical results of TSPO, Iba-1, NLRP3 expression (Under 40× optical microscope). Brown cells are positive (as shown by the black arrow) and blue cells are negative.

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