ENTPD2 Antibody | Affinity Biosciences

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Western blot analysis of ENTPD2 using HuvEc whole cell lysates.

Product:	ENTPD2 Antibody
Catalog:	DF8194
Description:	Rabbit polyclonal antibody to ENTPD2
Application:	WB
Reactivity:	Human
Prediction:	Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.:	54 kDa; 54kD(Calculated).
Uniprot:	Q9Y5L3
RRID:	AB_2841503

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(89%), Bovine(100%), Horse(89%), Sheep(89%), Dog(89%)

Clonality:

Polyclonal

Specificity:

ENTPD2 Antibody detects endogenous levels of total ENTPD2.

RRID:

AB_2841503
Cite Format: Affinity Biosciences Cat# DF8194, RRID:AB_2841503.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

NTPDase 2; NTPDase2; CD39 antigen like 1; CD39 antigen-like 1; CD39 like1; CD39L1; CD39like1; ecto ATP diphosphohydrolase 2; ecto ATPase 2; ecto ATPDase 2; Ecto-ATP diphosphohydrolase 2; Ecto-ATPase 2; Ecto-ATPDase 2; ectoATPase 2; ectoATPDase 2; Ectonucleoside triphosphate diphosphohydrolase 2; ENTP2_HUMAN; Entpd2; NTPDase 2;

Immunogens

Immunogen:

Uniprot:

Q9Y5L3(ENTP2_HUMAN) >>Visit HPA database.

Gene(ID):

ENTPD2(954)

Expression:

Q9Y5L3 ENTP2_HUMAN:

Brain, placenta, skeletal muscle, kidney, pancreas, heart, ovary, testis, colon, small intestine, prostate and pancreas. No expression in adult thymus, spleen, lung, liver and peripheral blood leukocytes.

Sequence:

Q9Y5L3 ENTP2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAGKVRSLLPPLLLAAAGLAGLLLLCVPTRDVREPPALKYGIVLDAGSSHTSMFIYKWPADKENDTGIVGQHSSCDVPGGGISSYADNPSGASQSLVGCLEQALQDVPKERHAGTPLYLGATAGMRLLNLTNPEASTSVLMAVTHTLTQYPFDFRGARILSGQEEGVFGWVTANYLLENFIKYGWVGRWFRPRKGTLGAMDLGGASTQITFETTSPAEDRASEVQLHLYGQHYRVYTHSFLCYGRDQVLQRLLASALQTHGFHPCWPRGFSTQVLLGDVYQSPCTMAQRPQNFNSSARVSLSGSSDPHLCRDLVSGLFSFSSCPFSRCSFNGVFQPPVAGNFVAFSAFFYTVDFLRTSMGLPVATLQQLEAAAVNVCNQTWAQLQARVPGQRARLADYCAGAMFVQQLLSRGYGFDERAFGGVIFQKKAADTAVGWALGYMLNLTNLIPADPPGLRKGTDFSSWVVLLLLFASALLAALVLLLRQVHSAKLPSTI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

100

Pig

Horse

Sheep

Dog

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y5L3 As Substrate

Q9Y5L3

Site	PTM Type	Source
T196	Phosphorylation	Uniprot
S215	Phosphorylation	Uniprot
N443	N-Glycosylation	Uniprot

Research Backgrounds

Q9Y5L3_ENTP2_HUMAN

Function:

In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Hydrolyzes ADP only to a marginal extent. The order of activity with different substrates is ATP > GTP > CTP = ITP > UTP >> ADP = UDP.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Endoplasmic reticulum membrane>Multi-pass membrane protein.

Endoplasmic reticulum membrane>Multi-pass membrane protein.

Tissue Specificity:

Family&Domains:

Belongs to the GDA1/CD39 NTPase family.

Research Fields

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Organismal Systems > Sensory system > Taste transduction.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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ENTPD2 Antibody - #DF8194