alpha 2 Macroglobulin/A2M Antibody | Affinity Biosciences

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Western blot analysis of A2M using K562 whole cell lysates.

Product:	alpha 2 Macroglobulin/A2M Antibody
Catalog:	DF8174
Description:	Rabbit polyclonal antibody to alpha 2 Macroglobulin/A2M
Application:	WB
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	180 kDa; 163kD(Calculated).
Uniprot:	P01023
RRID:	AB_2841493

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

alpha 2 Macroglobulin/A2M Antibody detects endogenous levels of total alpha 2 Macroglobulin/A2M.

RRID:

AB_2841493
Cite Format: Affinity Biosciences Cat# DF8174, RRID:AB_2841493.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

A2m; A2MG_HUMAN; Alpha 2 M; Alpha 2M; Alpha-2-M; Alpha-2-macroglobulin; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5; CPAMD5; DKFZp779B086; FWP007; S863 7;

Immunogens

Immunogen:

Uniprot:

P01023(A2MG_HUMAN) >>Visit HPA database.

Gene(ID):

A2M(2)

Expression:

P01023 A2MG_HUMAN:

Secreted in plasma.

Sequence:

P01023 A2MG_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGKNKLLHPSLVLLLLVLLPTDASVSGKPQYMVLVPSLLHTETTEKGCVLLSYLNETVTVSASLESVRGNRSLFTDLEAENDVLHCVAFAVPKSSSNEEVMFLTVQVKGPTQEFKKRTTVMVKNEDSLVFVQTDKSIYKPGQTVKFRVVSMDENFHPLNELIPLVYIQDPKGNRIAQWQSFQLEGGLKQFSFPLSSEPFQGSYKVVVQKKSGGRTEHPFTVEEFVLPKFEVQVTVPKIITILEEEMNVSVCGLYTYGKPVPGHVTVSICRKYSDASDCHGEDSQAFCEKFSGQLNSHGCFYQQVKTKVFQLKRKEYEMKLHTEAQIQEEGTVVELTGRQSSEITRTITKLSFVKVDSHFRQGIPFFGQVRLVDGKGVPIPNKVIFIRGNEANYYSNATTDEHGLVQFSINTTNVMGTSLTVRVNYKDRSPCYGYQWVSEEHEEAHHTAYLVFSPSKSFVHLEPMSHELPCGHTQTVQAHYILNGGTLLGLKKLSFYYLIMAKGGIVRTGTHGLLVKQEDMKGHFSISIPVKSDIAPVARLLIYAVLPTGDVIGDSAKYDVENCLANKVDLSFSPSQSLPASHAHLRVTAAPQSVCALRAVDQSVLLMKPDAELSASSVYNLLPEKDLTGFPGPLNDQDNEDCINRHNVYINGITYTPVSSTNEKDMYSFLEDMGLKAFTNSKIRKPKMCPQLQQYEMHGPEGLRVGFYESDVMGRGHARLVHVEEPHTETVRKYFPETWIWDLVVVNSAGVAEVGVTVPDTITEWKAGAFCLSEDAGLGISSTASLRAFQPFFVELTMPYSVIRGEAFTLKATVLNYLPKCIRVSVQLEASPAFLAVPVEKEQAPHCICANGRQTVSWAVTPKSLGNVNFTVSAEALESQELCGTEVPSVPEHGRKDTVIKPLLVEPEGLEKETTFNSLLCPSGGEVSEELSLKLPPNVVEESARASVSVLGDILGSAMQNTQNLLQMPYGCGEQNMVLFAPNIYVLDYLNETQQLTPEIKSKAIGYLNTGYQRQLNYKHYDGSYSTFGERYGRNQGNTWLTAFVLKTFAQARAYIFIDEAHITQALIWLSQRQKDNGCFRSSGSLLNNAIKGGVEDEVTLSAYITIALLEIPLTVTHPVVRNALFCLESAWKTAQEGDHGSHVYTKALLAYAFALAGNQDKRKEVLKSLNEEAVKKDNSVHWERPQKPKAPVGHFYEPQAPSAEVEMTSYVLLAYLTAQPAPTSEDLTSATNIVKWITKQQNAQGGFSSTQDTVVALHALSKYGAATFTRTGKAAQVTIQSSGTFSSKFQVDNNNRLLLQQVSLPELPGEYSMKVTGEGCVYLQTSLKYNILPEKEEFPFALGVQTLPQTCDEPKAHTSFQISLSVSYTGSRSASNMAIVDVKMVSGFIPLKPTVKMLERSNHVSRTEVSSNHVLIYLDKVSNQTLSLFFTVLQDVPVRDLKPAIVKVYDYYETDEFAIAEYNAPCSKDLGNA

PTMs - P01023 As Substrate

P01023

Site	PTM Type	Source
N55	N-Glycosylation	Uniprot
N70	N-Glycosylation	Uniprot
N247	N-Glycosylation	Uniprot
T346	Phosphorylation	Uniprot
N396	N-Glycosylation	Uniprot
N410	N-Glycosylation	Uniprot
Y497	Phosphorylation	Uniprot
T679	Phosphorylation	Uniprot
Y695	Phosphorylation	Uniprot
Y708	Phosphorylation	Uniprot
S710	Phosphorylation	Uniprot
Y817	Phosphorylation	Uniprot
K820	Acetylation	Uniprot
T861	Phosphorylation	Uniprot
N869	N-Glycosylation	Uniprot
T898	Phosphorylation	Uniprot
N991	N-Glycosylation	Uniprot
Y1007	Phosphorylation	Uniprot
Y1012	Phosphorylation	Uniprot
Y1021	Phosphorylation	Uniprot
Y1025	Phosphorylation	Uniprot
K1147	Acetylation	Uniprot
K1162	Acetylation	Uniprot
K1164	Acetylation	Uniprot
K1168	Acetylation	Uniprot
S1387	Phosphorylation	Uniprot
T1395	Phosphorylation	Uniprot
N1424	N-Glycosylation	Uniprot

Research Backgrounds

P01023_A2MG_HUMAN

Function:

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region, a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

Subcellular Location:

Secreted.

Tissue Specificity:

Secreted in plasma.

Subunit Structure:

Homotetramer; disulfide-linked.

Family&Domains:

Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.

Research Fields

· Organismal Systems > Immune system > Complement and coagulation cascades. (View pathway)

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alpha 2 Macroglobulin/A2M Antibody - #DF8174