BORA Antibody - #DF8147
(1)
| Product: | BORA Antibody |
| Catalog: | DF8147 |
| Description: | Rabbit polyclonal antibody to BORA |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus |
| Mol.Wt.: | 61kDa; 61kD(Calculated). |
| Uniprot: | Q6PGQ7 |
| RRID: | AB_2841478 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:200-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse
Prediction:
Pig(88%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(86%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
BORA Antibody detects endogenous levels of total BORA.
RRID:
AB_2841478
Cite Format: Affinity Biosciences Cat# DF8147, RRID:AB_2841478.
Cite Format: Affinity Biosciences Cat# DF8147, RRID:AB_2841478.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
Aurora borealis; bora; bora, aurora kinase A activator; BORA_HUMAN; C13orf34; Chromosome 13 open reading frame 34; FLJ22624; HsBora; Protein aurora borealis; RP11-342J4.2;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Sequence:
- Q6PGQ7 BORA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGDVKESKMQITPETPGRIPVLNPFESPSDYSNLHEQTLASPSVFKSTKLPTPGKFRWSIDQLAVINPVEIDPEDIHRQALYLSHSRIDKDVEDKRQKAIEEFFTKDVIVPSPWTDHEGKQLSQCHSSKCTNINSDSPVGKKLTIHSEKSDAACQTLLSLPVDFNLENILGDYFRADEFADQSPGNLSSSSLRRKLFLDGNGSISDSLPSASPGSPHSGVQTSLEMFYSIDLSPVKCRSPLQTPSSGQFSSSPIQASAKKYSLGSITSPSPISSPTFSPIEFQIGETPLSEQRKFTVHSPDASSGTNSNGITNPCIRSPYIDGCSPIKNWSPMRLQMYSGGTQYRTSVIQIPFTLETQGEDEEDKENIPSTDVSSPAMDAAGIHLRQFSNEASTHGTHLVVTAMSVTQNQSSASEKELALLQDVEREKDNNTVDMVDPIEIADETTWIKEPVDNGSLPMTDFVSGIAFSIENSHMCMSPLAESSVIPCESSNIQMDSGYNTQNCGSNIMDTVGAESYCKESDAQTCEVESKSQAFNMKQDHTTQRCWMKTASPFQCSSP
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Xenopus
100
Chicken
100
Pig
88
Rabbit
86
Zebrafish
75
Dog
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q6PGQ7 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y31 | Phosphorylation | Uniprot | |
| T38 | Phosphorylation | Uniprot | |
| S41 | Phosphorylation | Uniprot | |
| K46 | Ubiquitination | Uniprot | |
| T52 | Phosphorylation | Uniprot | |
| Y82 | Phosphorylation | Uniprot | |
| K95 | Acetylation | Uniprot | |
| K98 | Acetylation | Uniprot | |
| K106 | Acetylation | Uniprot | |
| K106 | Ubiquitination | Uniprot | |
| S112 | Phosphorylation | Uniprot | |
| K120 | Ubiquitination | Uniprot | |
| S135 | Phosphorylation | Uniprot | |
| S137 | Phosphorylation | Uniprot | |
| K142 | Ubiquitination | Uniprot | |
| S183 | Phosphorylation | Uniprot | |
| S188 | Phosphorylation | Uniprot | |
| S189 | Phosphorylation | Uniprot | |
| S190 | Phosphorylation | Uniprot | |
| S191 | Phosphorylation | Uniprot | |
| S239 | Phosphorylation | Uniprot | |
| S252 | Phosphorylation | P06493 (CDK1) | Uniprot |
| S270 | Phosphorylation | Uniprot | |
| S274 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| S278 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| T296 | Phosphorylation | Uniprot | |
| S299 | Phosphorylation | Uniprot | |
| S303 | Phosphorylation | Uniprot | |
| S318 | Phosphorylation | Uniprot | |
| S325 | Phosphorylation | P50750 (CDK9) | Uniprot |
| S331 | Phosphorylation | Uniprot | |
| S339 | Phosphorylation | Uniprot | |
| T432 | Phosphorylation | Uniprot | |
| T445 | Phosphorylation | Uniprot | |
| T446 | Phosphorylation | Uniprot | |
| S497 | Phosphorylation | P53350 (PLK1) | Uniprot |
| T501 | Phosphorylation | Q13535 (ATR) , P53350 (PLK1) | Uniprot |
| K531 | Ubiquitination | Uniprot | |
| K538 | Ubiquitination | Uniprot | |
| S552 | Phosphorylation | Uniprot | |
| S558 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
Required for the activation of AURKA at the onset of mitosis.
PTMs:
Phosphorylated by AURKA.
Subunit Structure:
Interacts with AURKA.
Family&Domains:
Belongs to the BORA family.
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