PNPLA2 Antibody - #DF8138
| Product: | PNPLA2 Antibody |
| Catalog: | DF8138 |
| Description: | Rabbit polyclonal antibody to PNPLA2 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human |
| Mol.Wt.: | 54 kDa; 55kD(Calculated). |
| Uniprot: | Q96AD5 |
| RRID: | AB_2841473 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF8138, RRID:AB_2841473.
Fold/Unfold
1110001C14Rik; Adipose triglyceride lipase; ATGL; ATGL DESNUTRIN; Calcium independent phospholipase A2; Calcium-independent phospholipase A2; Desnutrin; EC 3.1.1.3; FP17548; IPLA2 zeta; IPLA2-zeta; Mutant patatin like phospholipase domain containing 2; Patatin like phospholipase domain containing 2; PATATIN LIKE PHOSPHOLIPASE DOMAIN CONTAINING PROTEIN 2; Patatin-like phospholipase domain-containing protein 2; PEDF R; PHOSPHOLIPASE A2 CALCIUM INDEPENDENT ZETA; Pigment epithelium derived factor; Pigment epithelium-derived factor; plpl; plpl2; PLPL2_HUMAN; Pnpla2; Transport secretion protein 2; Transport secretion protein 2.2; Transport-secretion protein 2; Triglyceride hydrolase; TTS 2.2; TTS2; TTS2.2; ZETA;
Immunogens
Highest expression in adipose tissue. Also detected in heart, skeletal muscle, and portions of the gastrointestinal tract. Detected in normal retina and retinoblastoma cells. Detected in retinal pigment epithelium and, at lower intensity, in the inner segments of photoreceptors and in the ganglion cell layer of the neural retina (at protein level).
- Q96AD5 PLPL2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MFPREKTWNISFAGCGFLGVYYVGVASCLREHAPFLVANATHIYGASAGALTATALVTGVCLGEAGAKFIEVSKEARKRFLGPLHPSFNLVKIIRSFLLKVLPADSHEHASGRLGISLTRVSDGENVIISHFNSKDELIQANVCSGFIPVYCGLIPPSLQGVRYVDGGISDNLPLYELKNTITVSPFSGESDICPQDSSTNIHELRVTNTSIQFNLRNLYRLSKALFPPEPLVLREMCKQGYRDGLRFLQRNGLLNRPNPLLALPPARPHGPEDKDQAVESAQAEDYSQLPGEDHILEHLPARLNEALLEACVEPTDLLTTLSNMLPVRLATAMMVPYTLPLESALSFTIRLLEWLPDVPEDIRWMKEQTGSICQYLVMRAKRKLGRHLPSRLPEQVELRRVQSLPSVPLSCAAYREALPGWMRNNLSLGDALAKWEECQRQLLLGLFCTNVAFPPEALRMRAPADPAPAPADPASPQHQLAGPAPLLSTPAPEARPVIGALGL
PTMs - Q96AD5 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K74 | Ubiquitination | Uniprot | |
| K92 | Ubiquitination | Uniprot | |
| K100 | Ubiquitination | Uniprot | |
| K224 | Ubiquitination | Uniprot | |
| Y287 | Phosphorylation | Uniprot | |
| K367 | Ubiquitination | Uniprot | |
| S391 | Phosphorylation | Uniprot | |
| S404 | Phosphorylation | Uniprot | |
| S407 | Phosphorylation | Uniprot | |
| S411 | Phosphorylation | Uniprot | |
| S428 | Phosphorylation | Uniprot | |
| K435 | Ubiquitination | Uniprot | |
| S476 | Phosphorylation | Uniprot | |
| T490 | Phosphorylation | Uniprot |
Research Backgrounds
Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. Also has acylglycerol transacylase activity. May act coordinately with LIPE/HLS within the lipolytic cascade. Regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion.
Phosphorylation at Ser-404 by PKA is increased during fasting and moderate intensity exercise, and moderately increases lipolytic activity (By similarity). Phosphorylation at Ser-404 is increased upon beta-adrenergic stimulation.
Lipid droplet. Cell membrane>Single-pass type II membrane protein.
Highest expression in adipose tissue. Also detected in heart, skeletal muscle, and portions of the gastrointestinal tract. Detected in normal retina and retinoblastoma cells. Detected in retinal pigment epithelium and, at lower intensity, in the inner segments of photoreceptors and in the ganglion cell layer of the neural retina (at protein level).
Interacts with ABHD5; this association stimulates PNPLA2 triglyceride hydrolase activity (By similarity). Interacts with SERPINF1; interacts at one site of interaction. Despite a colocalization in lipid droplets, it probably does not interact with PLIN (By similarity). Interacts with PLIN5; prevents interaction with ABHD5 (By similarity). Interacts with FAF2.
Research Fields
· Metabolism > Lipid metabolism > Glycerolipid metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.
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