FDFT1 Antibody | Affinity Biosciences

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Western blot analysis FDFT1 using A549 whole cell lysates.

DF8121 at 1/100 staining Mouse muscle tissue by IHC-P.

Product:	FDFT1 Antibody
Catalog:	DF8121
Description:	Rabbit polyclonal antibody to FDFT1
Application:	WB IHC
Reactivity:	Human
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	48 kDa; 48kD(Calculated).
Uniprot:	P37268
RRID:	AB_2841461

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(92%), Bovine(83%), Horse(92%), Sheep(92%), Rabbit(100%), Dog(92%), Chicken(92%)

Clonality:

Polyclonal

Specificity:

FDFT1 Antibody detects endogenous levels of total FDFT1.

RRID:

AB_2841461
Cite Format: Affinity Biosciences Cat# DF8121, RRID:AB_2841461.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

DGPT; ERG9; Farnesyl diphosphate farnesyltransferase; Farnesyl-diphosphate farnesyltransferase; FDFT_HUMAN; FDFT1; FPP:FPP farnesyltransferase; SQS; Squalene synthase; Squalene synthetase; SS;

Immunogens

Immunogen:

Uniprot:

P37268(FDFT_HUMAN) >>Visit HPA database.

Gene(ID):

FDFT1(2222)

Expression:

P37268 FDFT_HUMAN:

Widely expressed.

Sequence:

P37268 FDFT_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEFVKCLGHPEEFYNLVRFRIGGKRKVMPKMDQDSLSSSLKTCYKYLNQTSRSFAAVIQALDGEMRNAVCIFYLVLRALDTLEDDMTISVEKKVPLLHNFHSFLYQPDWRFMESKEKDRQVLEDFPTISLEFRNLAEKYQTVIADICRRMGIGMAEFLDKHVTSEQEWDKYCHYVAGLVGIGLSRLFSASEFEDPLVGEDTERANSMGLFLQKTNIIRDYLEDQQGGREFWPQEVWSRYVKKLGDFAKPENIDLAVQCLNELITNALHHIPDVITYLSRLRNQSVFNFCAIPQVMAIATLAACYNNQQVFKGAVKIRKGQAVTLMMDATNMPAVKAIIYQYMEEIYHRIPDSDPSSSKTRQIISTIRTQNLPNCQLISRSHYSPIYLSFVMLLAALSWQYLTTLSQVTEDYVQTGEH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Pig

Horse

Sheep

Dog

Chicken

Bovine

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P37268 As Substrate

P37268

Site	PTM Type	Source
K5	Ubiquitination	Uniprot
C6	S-Nitrosylation	Uniprot
Y14	Phosphorylation	Uniprot
K30	Ubiquitination	Uniprot
K41	Ubiquitination	Uniprot
K45	Ubiquitination	Uniprot
K92	Ubiquitination	Uniprot
K93	Ubiquitination	Uniprot
K115	Ubiquitination	Uniprot
K117	Ubiquitination	Uniprot
T127	Phosphorylation	Uniprot
S129	Phosphorylation	Uniprot
K138	Ubiquitination	Uniprot
K160	Ubiquitination	Uniprot
S164	Phosphorylation	Uniprot
K213	Ubiquitination	Uniprot
K318	Ubiquitination	Uniprot
Y346	Phosphorylation	Uniprot
S352	Phosphorylation	Uniprot
K358	Ubiquitination	Uniprot

Research Backgrounds

P37268_FDFT_HUMAN

Subcellular Location:

Endoplasmic reticulum membrane>Multi-pass membrane protein.

Tissue Specificity:

Widely expressed.

Subunit Structure:

Monomer.

Family&Domains:

Belongs to the phytoene/squalene synthase family.

Research Fields

· Metabolism > Lipid metabolism > Steroid biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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FDFT1 Antibody - #DF8121