XDH Antibody | Affinity Biosciences

WB
Cites

1/6

Western blot analysis of extracts from colo236(heat-shock treatment), using XDH Antibody at 1/1000 dilution.

Fig.

Figure 2 C1QBP regulates the expression of XDH in RCC.

Product:	XDH Antibody
Catalog:	DF8111
Description:	Rabbit polyclonal antibody to XDH
Application:	WB IHC
Reactivity:	Human, Rat
Prediction:	Pig, Bovine, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	146 kDa; 146kD(Calculated).
Uniprot:	P47989
RRID:	AB_2841454

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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MS Report

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MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Rat

Prediction:

Pig(88%), Bovine(100%), Sheep(88%), Rabbit(100%), Dog(100%), Chicken(88%)

Clonality:

Polyclonal

Specificity:

XDH Antibody detects endogenous levels of total XDH.

RRID:

AB_2841454
Cite Format: Affinity Biosciences Cat# DF8111, RRID:AB_2841454.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Xanthine dehydrogenase; Xanthine dehydrogenase/oxidase; Xanthine oxidase; Xanthine oxidoreductase; XD; XDH; XDH_HUMAN; xdha; XO; xor;

Immunogens

Immunogen:

Uniprot:

P47989(XDH_HUMAN) >>Visit HPA database.

Gene(ID):

XDH(7498)

Expression:

P47989 XDH_HUMAN:

Detected in milk (at protein level).

Sequence:

P47989 XDH_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MTADKLVFFVNGRKVVEKNADPETTLLAYLRRKLGLSGTKLGCGEGGCGACTVMLSKYDRLQNKIVHFSANACLAPICSLHHVAVTTVEGIGSTKTRLHPVQERIAKSHGSQCGFCTPGIVMSMYTLLRNQPEPTMEEIENAFQGNLCRCTGYRPILQGFRTFARDGGCCGGDGNNPNCCMNQKKDHSVSLSPSLFKPEEFTPLDPTQEPIFPPELLRLKDTPRKQLRFEGERVTWIQASTLKELLDLKAQHPDAKLVVGNTEIGIEMKFKNMLFPMIVCPAWIPELNSVEHGPDGISFGAACPLSIVEKTLVDAVAKLPAQKTEVFRGVLEQLRWFAGKQVKSVASVGGNIITASPISDLNPVFMASGAKLTLVSRGTRRTVQMDHTFFPGYRKTLLSPEEILLSIEIPYSREGEYFSAFKQASRREDDIAKVTSGMRVLFKPGTTEVQELALCYGGMANRTISALKTTQRQLSKLWKEELLQDVCAGLAEELHLPPDAPGGMVDFRCTLTLSFFFKFYLTVLQKLGQENLEDKCGKLDPTFASATLLFQKDPPADVQLFQEVPKGQSEEDMVGRPLPHLAADMQASGEAVYCDDIPRYENELSLRLVTSTRAHAKIKSIDTSEAKKVPGFVCFISADDVPGSNITGICNDETVFAKDKVTCVGHIIGAVVADTPEHTQRAAQGVKITYEELPAIITIEDAIKNNSFYGPELKIEKGDLKKGFSEADNVVSGEIYIGGQEHFYLETHCTIAVPKGEAGEMELFVSTQNTMKTQSFVAKMLGVPANRIVVRVKRMGGGFGGKETRSTVVSTAVALAAYKTGRPVRCMLDRDEDMLITGGRHPFLARYKVGFMKTGTVVALEVDHFSNVGNTQDLSQSIMERALFHMDNCYKIPNIRGTGRLCKTNLPSNTAFRGFGGPQGMLIAECWMSEVAVTCGMPAEEVRRKNLYKEGDLTHFNQKLEGFTLPRCWEECLASSQYHARKSEVDKFNKENCWKKRGLCIIPTKFGISFTVPFLNQAGALLHVYTDGSVLLTHGGTEMGQGLHTKMVQVASRALKIPTSKIYISETSTNTVPNTSPTAASVSADLNGQAVYAACQTILKRLEPYKKKNPSGSWEDWVTAAYMDTVSLSATGFYRTPNLGYSFETNSGNPFHYFSYGVACSEVEIDCLTGDHKNLRTDIVMDVGSSLNPAIDIGQVEGAFVQGLGLFTLEELHYSPEGSLHTRGPSTYKIPAFGSIPIEFRVSLLRDCPNKKAIYASKAVGEPPLFLAASIFFAIKDAIRAARAQHTGNNVKELFRLDSPATPEKIRNACVDKFTTLCVTGVPENCKPWSVRV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

100

Dog

100

Rabbit

100

Pig

Sheep

Chicken

Zebrafish

Horse

Xenopus

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P47989 As Substrate

P47989

Site	PTM Type	Enzyme	Source
T39	Phosphorylation		Uniprot
T222	Phosphorylation	Q00535 (CDK5)	Uniprot
T311	Phosphorylation		Uniprot
T324	Phosphorylation		Uniprot
K443	Methylation		Uniprot
K552	Acetylation		Uniprot
S810	Phosphorylation		Uniprot
Y818	Phosphorylation		Uniprot
T856	Phosphorylation		Uniprot
S1052	Phosphorylation		Uniprot
S1111	Phosphorylation		Uniprot
S1113	Phosphorylation		Uniprot
T1320	Phosphorylation		Uniprot

Research Backgrounds

P47989_XDH_HUMAN

Function:

Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species. Has also low oxidase activity towards aldehydes (in vitro).

PTMs:

Subject to partial proteolysis; this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O).

Contains sulfhydryl groups that are easily oxidized (in vitro); this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O).

Subcellular Location:

Cytoplasm. Peroxisome. Secreted.

Tissue Specificity:

Detected in milk (at protein level).

Subunit Structure:

Homodimer. Interacts with BTN1A1 (By similarity).

Family&Domains:

Belongs to the xanthine dehydrogenase family.

Research Fields

· Cellular Processes > Transport and catabolism > Peroxisome. (View pathway)

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Biosynthesis of other secondary metabolites > Caffeine metabolism.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). Sesamol supplementation alleviates nonalcoholic steatohepatitis and atherosclerosis in high-fat, high carbohydrate and high-cholesterol diet-fed rats. Food & Function, 2021 (PubMed: 34606548) [IF=6.1]

Application: WB Species: Rat Sample: liver tissue

Fig. 6 Sesamol decreased uric acid levels through the inhibition of XO activity and/or its expression in HF-HCC diet-fed rats. (A–D) The analyses of serum and hepatic XO activities and uric acid levels (samples from 6 rats in each group were analyzed). (E) Representative images of the western blot analysis from hepatic XO expression, and (F) representative images of the IHC analyses from hepatic XO expression (samples from 3 rats in each group were analyzed). Values are expressed as means ± SEM (n = 6); #P < 0.05, # #P < 0.01 and ###P < 0.001, versus control; *P < 0.05 and **P < 0.01, versus HF-HCC.

Application: IHC Species: Rat Sample: liver tissue

Application: WB Species: Rat Sample:

Fig. 6 Sesamol decreased uric acid levels through the inhibition of XO activity and/or its expression in HF-HCC diet-fed rats. (A–D) The analyses of serum and hepatic XO activities and uric acid levels (samples from 6 rats in each group were analyzed). (E) Representative images of the western blot analysis from hepatic XO expression, and (F) representative images of the IHC analyses from hepatic XO expression (samples from 3 rats in each group were analyzed). Values are expressed as means ± SEM (n = 6); # P < 0.05, # # P < 0.01 and # # # P < 0.001, versus control; *P < 0.05 and **P < 0.01, versus HF-HCC.

2). C1QBP regulates apoptosis of renal cell carcinoma via modulating xanthine dehydrogenase (XDH) mediated ROS generation. International Journal of Medical Sciences, 2022 (PubMed: 35693733) [IF=3.6]

Application: WB Species: Human Sample: ACHN and 786-O cells

Figure 2 C1QBP regulates the expression of XDH in RCC. (A) Western blot detected the expression of XDH in ACHN and 786-O cells with stable C1QBP knockdown (left panel) and C1QBP overexpression (right panel). (B) The XDH mRNA was examined by qRT-PCR in ACHN (upper panel) and 786-O (lower panel) cells with stable C1QBP knockdown (left panel) and C1QBP overexpression (right panel). The expression of C1QBP and XDH was detected by western blot both in tumors (T) and adjacent normal tissues (N) of 30 primary RCC patients. (C) Representative 8 pairs of western blot images were shown. (D) The expression of C1QBP (left panel) and XDH (right panel) was normalized with β-actin and quantified by Image J software in 30 primary RCC patients. The analysis was used paired t-test, P = 0.015, P < 0.0001, respectively, n = 30. (E) The expression of C1QBP and XDH was examined by immunohistochemistry staining in a study cohort with 57 pairs of RCC (Tumor) and adjacent normal kidney tissues (Para-tumor). Representative pictures were shown, and brown signals indicated positive staining. Scale bar = 50μm. Statistically significant differences were indicated: *, P < 0.05, **, P < 0.01 and ***, P < 0.001.

Application: IHC Species: Human Sample: kidney tissues

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XDH Antibody - #DF8111