Product: HSPA2 Antibody
Catalog: DF8101
Description: Rabbit polyclonal antibody to HSPA2
Application: WB IHC
Reactivity: Human, Mouse, Monkey
Prediction: Pig, Bovine, Sheep, Rabbit, Dog
Mol.Wt.: 70 kDa; 70kD(Calculated).
Uniprot: P54652
RRID: AB_2841446

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Monkey
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(92%), Dog(100%)
Clonality:
Polyclonal
Specificity:
HSPA2 Antibody detects endogenous levels of total HSPA2.
RRID:
AB_2841446
Cite Format: Affinity Biosciences Cat# DF8101, RRID:AB_2841446.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

70kDa; Hcp70.2; Heat shock 70 kDa protein 2; Heat shock protein 2; Heat shock protein 70.2; Heat shock related 70 kDa protein 2; Heat shock-related 70 kDa protein 2; Heat-shock protein, 70-KD, 2; Heat-shock protein, 70-KD, 3; HSP70 2; HSP70 3; Hsp70-2; HSP70-3; HSP70.2; HSP70A2; HSP72; HSP72_HUMAN; HSPA2; Hspt70; Hst70; MGC58299; MGC7795; MGC93458; OTTHUMP00000180664; Testis-specific heat shock protein-related;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MSARGPAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTIFDAKRLIGRKFEDATVQSDMKHWPFRVVSEGGKPKVQVEYKGETKTFFPEEISSMVLTKMKEIAEAYLGGKVHSAVITVPAYFNDSQRQATKDAGTITGLNVLRIINEPTAAAIAYGLDKKGCAGGEKNVLIFDLGGGTFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVSHLAEEFKRKHKKDIGPNKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGVDFYTSITRARFEELNADLFRGTLEPVEKALRDAKLDKGQIQEIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILIGDKSENVQDLLLLDVTPLSLGIETAGGVMTPLIKRNTTIPTKQTQTFTTYSDNQSSVLVQVYEGERAMTKDNNLLGKFDLTGIPPAPRGVPQIEVTFDIDANGILNVTAADKSTGKENKITITNDKGRLSKDDIDRMVQEAERYKSEDEANRDRVAAKNALESYTYNIKQTVEDEKLRGKISEQDKNKILDKCQEVINWLDRNQMAEKDEYEHKQKELERVCNPIISKLYQGGPGGGSGGGGSGASGGPTIEEVD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
92
Xenopus
67
Horse
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P54652 As Substrate

Site PTM Type Enzyme
Y16 Phosphorylation
K26 Ubiquitination
T38 Phosphorylation
T39 Phosphorylation
S41 Phosphorylation
Y42 Phosphorylation
T46 Phosphorylation
T48 Phosphorylation
R50 Methylation
K57 Acetylation
T65 Phosphorylation
T67 Phosphorylation
K72 Acetylation
K78 Ubiquitination
K101 Ubiquitination
K103 Ubiquitination
Y108 Phosphorylation
K109 Acetylation
K109 Ubiquitination
T112 Phosphorylation
K113 Ubiquitination
S121 Phosphorylation
S122 Phosphorylation
T126 Phosphorylation
T159 Phosphorylation
T164 Phosphorylation
T166 Phosphorylation
T178 Phosphorylation
Y184 Phosphorylation
K188 Acetylation
K188 Ubiquitination
K189 Ubiquitination
K223 Ubiquitination
S224 Phosphorylation
T225 Phosphorylation
T229 Phosphorylation
K249 Acetylation
T268 Phosphorylation
T276 Phosphorylation
S280 Phosphorylation
T301 Phosphorylation
K322 Acetylation
K328 Ubiquitination
K348 Ubiquitination
K351 Acetylation
K351 Ubiquitination
K360 Acetylation
K360 Sumoylation
K360 Ubiquitination
K364 Sumoylation
K364 Ubiquitination
S365 Phosphorylation
T421 Phosphorylation
K454 Acetylation
K454 Ubiquitination
T465 Phosphorylation
R472 Methylation
T498 Phosphorylation
K500 Ubiquitination
K503 Ubiquitination
T505 Phosphorylation
K510 Acetylation
K510 Sumoylation
K510 Ubiquitination
K515 Acetylation
R520 Methylation
R527 Methylation
K564 Methylation
T634 Phosphorylation

Research Backgrounds

Function:

Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The affinity for polypeptides is regulated by its nucleotide bound state. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. It goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release. Plays a role in spermatogenesis. In association with SHCBP1L may participate in the maintenance of spindle integrity during meiosis in male germ cells (By similarity).

Subcellular Location:

Cytoplasm>Cytoskeleton>Spindle.
Note: Colocalizes with SHCBP1L at spindle during the meiosis process.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with FKBP6. Interacts with ZNF541. Component of the CatSper complex. Interacts with RABL2/RABL2A; binds preferentially to GTP-bound RABL2. Interacts with SHCBP1L; this interaction may promote the recruitment of HSPA2 to the spindle (By similarity). Interacts with FKBP6.

Family&Domains:

The N-terminal nucleotide binding domain (NBD) (also known as the ATPase domain) is responsible for binding and hydrolyzing ATP. The C-terminal substrate-binding domain (SBD) (also known as peptide-binding domain) binds to the client/substrate proteins. The two domains are allosterically coupled so that, when ATP is bound to the NBD, the SBD binds relatively weakly to clients. When ADP is bound in the NBD, a conformational change enhances the affinity of the SBD for client proteins.

Belongs to the heat shock protein 70 family.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Genetic Information Processing > Transcription > Spliceosome.

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Organismal Systems > Aging > Longevity regulating pathway - multiple species.   (View pathway)

· Organismal Systems > Immune system > Antigen processing and presentation.   (View pathway)

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

References

1). Melatonin improves the ability of spermatozoa to bind with oocytes in the mouse. REPRODUCTION FERTILITY AND DEVELOPMENT, 2023 (PubMed: 37068786) [IF=1.9]

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