EVL Antibody - #DF8091

(2)  
Product: EVL Antibody
Catalog: DF8091
Description: Rabbit polyclonal antibody to EVL
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Dog
Mol.Wt.: 50 kDa; 45kD(Calculated).
Uniprot: Q9UI08
RRID: AB_2841440

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(80%), Horse(100%), Dog(90%)
Clonality:
Polyclonal
Specificity:
EVL Antibody detects endogenous levels of total EVL.
RRID:
AB_2841440
Cite Format: Affinity Biosciences Cat# DF8091, RRID:AB_2841440.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AI528774; Ena-vasodilator stimulated phosphoprotein; Ena/vasodilator stimulated phosphoprotein like; Ena/VASP like protein; EVL; RNB 6; RNB6;

Immunogens

Immunogen:
Uniprot:

Q9UI08(EVL_HUMAN) >>Visit HPA database.

Gene(ID):
EVL(51466)
Sequence:
MSEQSICQARASVMVYDDTSKKWVPIKPGQQGFSRINIYHNTASNTFRVVGVKLQDQQVVINYSIVKGLKYNQATPTFHQWRDARQVYGLNFASKEEATTFSNAMLFALNIMNSQEGGPSSQRQVQNGPSPDEMDIQRRQVMEQHQQQRQESLERRTSATGPILPPGHPSSAASAPVSCSGPPPPPPPPVPPPPTGATPPPPPPLPAGGAQGSSHDESSMSGLAAAIAGAKLRRVQRPEDASGGSSPSGTSKSDANRASSGGGGGGLMEEMNKLLAKRRKAASQSDKPAEKKEDESQMEDPSTSPSPGTRAASQPPNSSEAGRKPWERSNSVEKPVSSILSRTPSVAKSPEAKSPLQSQPHSRMKPAGSVNDMALDAFDLDRMKQEILEEVVRELHKVKEEIIDAIRQELSGISTT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
90
Bovine
80
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UI08 As Substrate

Site PTM Type Enzyme
Y16 Phosphorylation
S20 Phosphorylation
K27 Ubiquitination
Y39 Phosphorylation
S130 Phosphorylation
S152 Phosphorylation
S158 Phosphorylation
S171 Phosphorylation
S242 Phosphorylation
S246 Phosphorylation
S248 Phosphorylation
T250 Phosphorylation
K252 Ubiquitination
S253 Phosphorylation
S259 Phosphorylation
S260 Phosphorylation
K280 Ubiquitination
S283 Phosphorylation
S296 Phosphorylation
T303 Phosphorylation
S304 Phosphorylation
S306 Phosphorylation
S313 Phosphorylation
S329 Phosphorylation
S331 Phosphorylation
S337 Phosphorylation
S338 Phosphorylation
S341 Phosphorylation
T343 Phosphorylation
S345 Phosphorylation
S349 Phosphorylation
S354 Phosphorylation
S358 Phosphorylation
S362 Phosphorylation
K365 Ubiquitination
S369 Phosphorylation
K399 Ubiquitination

Research Backgrounds

Function:

Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. EVL enhances actin nucleation and polymerization.

PTMs:

Phosphorylated by PKA; phosphorylation abolishes binding to SH3 domains of ABL and SRC.

Subcellular Location:

Cytoplasm>Cytoskeleton. Cytoplasm>Cytoskeleton>Stress fiber. Cell projection>Lamellipodium.
Note: Targeted to the leading edge of lamellipodia and the distal tip of stress fibers through interaction with a number of proteins. In activated T-cells, localizes to the F-actin collar and the distal tip of microspikes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homotetramer (By similarity). Binds to the SH3 domains of ABL1, LYN and SRC. Also binds to profilin, with preference for isoform IIa of PFN2, and the WW domain of APBB1/FE65. Binds to SEMA6A. Interacts, via the Pro-rich region, with the C-terminal SH3 domain of DNMBP. Interacts with RAPH1. Binds, via the EVH1 domain, the Pro-rich domain of Listeria monocytogenes actA (By similarity). Binds, via the EVH1 domain, the Pro-rich domain of ZYX. Interacts with FYB1. Interacts with ZDHHC17.

Family&Domains:

The EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding. The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is required for F-actin binding and subcellular location, and Block C for tetramerization.

Belongs to the Ena/VASP family.

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