TRIP10 Antibody - #DF8087

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Product: TRIP10 Antibody
Catalog: DF8087
Description: Rabbit polyclonal antibody to TRIP10
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Dog
Mol.Wt.: 68 kDa; 68kD(Calculated).
Uniprot: Q15642
RRID: AB_2841438

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Bovine(92%), Horse(92%), Dog(92%)
Clonality:
Polyclonal
Specificity:
TRIP10 Antibody detects endogenous levels of total TRIP10.
RRID:
AB_2841438
Cite Format: Affinity Biosciences Cat# DF8087, RRID:AB_2841438.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cdc42 interacting protein 4; Cdc42 interaction protein 4 long isoform; Cdc42-interacting protein 4; CG11341; CG15015 PA; Cip 4; CIP4; CIP4_HUMAN; DCIP4; hSTP; Protein Felic; Salt tolerant protein; Salt tolerator; STOT; STP; Thyroid hormone receptor interactor 10; Thyroid receptor interacting protein 10; Thyroid receptor-interacting protein 10; TR-interacting protein 10; TRIP 10; TRIP-10; trip10; Truncated Cdc42 interaction protein 4;

Immunogens

Immunogen:
Uniprot:

Q15642(CIP4_HUMAN) >>Visit HPA database.

Gene(ID):
TRIP10(9322)
Expression:
Q15642 CIP4_HUMAN:

Expressed in brain, colon, heart, kidney, liver, lung, megakaryocyte, ovary, pancreas, peripheral blood lymphocytes, placenta, prostate, skeletal muscle, small intestine, spleen, testis, thymus and trachea.

Sequence:
MDWGTELWDQFEVLERHTQWGLDLLDRYVKFVKERTEVEQAYAKQLRSLVKKYLPKRPAKDDPESKFSQQQSFVQILQEVNDFAGQRELVAENLSVRVCLELTKYSQEMKQERKMHFQEGRRAQQQLENGFKQLENSKRKFERDCREAEKAAQTAERLDQDINATKADVEKAKQQAHLRSHMAEESKNEYAAQLQRFNRDQAHFYFSQMPQIFDKLQDMDERRATRLGAGYGLLSEAELEVVPIIAKCLEGMKVAANAVDPKNDSHVLIELHKSGFARPGDVEFEDFSQPMNRAPSDSSLGTPSDGRPELRGPGRSRTKRWPFGKKNKPRPPPLSPLGGPVPSALPNGPPSPRSGRDPLAILSEISKSVKPRLASFRSLRGSRGTVVTEDFSHLPPEQQRKRLQQQLEERSRELQKEVDQREALKKMKDVYEKTPQMGDPASLEPQIAETLSNIERLKLEVQKYEAWLAEAESRVLSNRGDSLSRHARPPDPPASAPPDSSSNSASQDTKESSEEPPSEESQDTPIYTEFDEDFEEEPTSPIGHCVAIYHFEGSSEGTISMAEGEDLSLMEEDKGDGWTRVRRKEGGEGYVPTSYLRVTLN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
92
Horse
92
Bovine
92
Dog
92
Zebrafish
67
Sheep
0
Xenopus
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15642 As Substrate

Site PTM Type Enzyme
R27 Methylation
K44 Ubiquitination
K104 Acetylation
K132 Acetylation
K132 Ubiquitination
K166 Ubiquitination
K187 Ubiquitination
Y190 Phosphorylation
K215 Ubiquitination
S235 Phosphorylation
S288 Phosphorylation
S296 Phosphorylation
S298 Phosphorylation
S299 Phosphorylation
T302 Phosphorylation
S304 Phosphorylation
S335 Phosphorylation
S343 Phosphorylation
S351 Phosphorylation
S363 Phosphorylation
S375 Phosphorylation
S378 Phosphorylation
S442 Phosphorylation
K458 Ubiquitination
S477 Phosphorylation
S482 Phosphorylation
T579 Phosphorylation
Y590 Phosphorylation

Research Backgrounds

Function:

Required for translocation of GLUT4 to the plasma membrane in response to insulin signaling (By similarity). Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. Binds to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promotes membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by recruiting WASL/N-WASP which in turn activates the Arp2/3 complex. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. Required for the formation of podosomes, actin-rich adhesion structures specific to monocyte-derived cells. May be required for the lysosomal retention of FASLG/FASL.

PTMs:

Tyrosine phosphorylated. Also phosphorylated by PKA.

Subcellular Location:

Cytoplasm>Cytoskeleton. Cytoplasm>Cell cortex. Lysosome. Golgi apparatus. Cell membrane. Cell projection>Phagocytic cup.
Note: Translocates to the plasma membrane in response to insulin stimulation, and this may require active RHOQ (By similarity). Localizes to cortical regions coincident with F-actin, to lysosomes and to sites of phagocytosis in macrophages. Also localizes to the Golgi, and this requires AKAP9.

Cytoplasm>Perinuclear region.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in brain, colon, heart, kidney, liver, lung, megakaryocyte, ovary, pancreas, peripheral blood lymphocytes, placenta, prostate, skeletal muscle, small intestine, spleen, testis, thymus and trachea.

Subunit Structure:

Interacts specifically with GTP-bound RHOQ. Interacts with DNM2 and PDE6G (By similarity). Homodimerizes, the dimers can polymerize end-to-end to form filamentous structures. Interacts specifically with GTP-bound CDC42. Interacts with AKAP9, ARHGAP17, DAAM1, DIAPH1, DIAPH2, DNM1, FASLG/FASL, GAPVD1, LYN, microtubules, SRC, WAS/WASP and WASL/N-WASP. Interacts with the ligand binding domain of the thyroid receptor (TR) in the presence of thyroid hormone. May interact with CTNNB1 and HD/HTT.

Family&Domains:

The F-BAR domain binds the phospholipid membrane with its concave surface. The end-to-end polymerization of dimers of these domains provides a curved surface that fits best membranes with around 600 A diameter, and may drive tubulation.

Belongs to the FNBP1 family.

Research Fields

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

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