STAMBP Antibody - #DF8083

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Product: STAMBP Antibody
Catalog: DF8083
Description: Rabbit polyclonal antibody to STAMBP
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Xenopus
Mol.Wt.: 50 kDa; 48kD(Calculated).
Uniprot: O95630
RRID: AB_2841436

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Xenopus(88%)
Clonality:
Polyclonal
Specificity:
STAMBP Antibody detects endogenous levels of total STAMBP.
RRID:
AB_2841436
Cite Format: Affinity Biosciences Cat# DF8083, RRID:AB_2841436.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AMSH; Associated molecule with the SH3 domain of STAM; Endosome associated ubiquitin isopeptidase; Endosome-associated ubiquitin isopeptidase; MGC126516; MGC126518; MICCAP; STABP_HUMAN; STAM binding protein; STAM-binding protein; Stambp;

Immunogens

Immunogen:
Uniprot:

O95630(STABP_HUMAN) >>Visit HPA database.

Gene(ID):
STAMBP(10617)
Expression:
O95630 STABP_HUMAN:

Ubiquitously expressed.

Sequence:
MSDHGDVSLPPEDRVRALSQLGSAVEVNEDIPPRRYFRSGVEIIRMASIYSEEGNIEHAFILYNKYITLFIEKLPKHRDYKSAVIPEKKDTVKKLKEIAFPKAEELKAELLKRYTKEYTEYNEEKKKEAEELARNMAIQQELEKEKQRVAQQKQQQLEQEQFHAFEEMIRNQELEKERLKIVQEFGKVDPGLGGPLVPDLEKPSLDVFPTLTVSSIQPSDCHTTVRPAKPPVVDRSLKPGALSNSESIPTIDGLRHVVVPGRLCPQFLQLASANTARGVETCGILCGKLMRNEFTITHVLIPKQSAGSDYCNTENEEELFLIQDQQGLITLGWIHTHPTQTAFLSSVDLHTHCSYQMMLPESVAIVCSPKFQETGFFKLTDHGLEEISSCRQKGFHPHSKDPPLFCSCSHVTVVDRAVTITDLR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Xenopus
88
Dog
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95630 As Substrate

Site PTM Type Enzyme
S2 Phosphorylation
S19 Phosphorylation
S39 Phosphorylation
S48 Phosphorylation
K81 Ubiquitination
K88 Acetylation
K88 Ubiquitination
K96 Ubiquitination
K102 Sumoylation
K102 Ubiquitination
K107 Ubiquitination
K112 Ubiquitination
K116 Ubiquitination
K144 Ubiquitination
K153 Acetylation
K153 Ubiquitination
K176 Ubiquitination
K180 Ubiquitination
K187 Ubiquitination
K238 Ubiquitination
S243 Phosphorylation
S245 Phosphorylation
S247 Phosphorylation
R255 Methylation
K378 Ubiquitination
K393 Ubiquitination
S399 Phosphorylation
R424 Methylation

Research Backgrounds

Function:

Zinc metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not cleave 'Lys-48'-linked polyubiquitin chains (By similarity). Plays a role in signal transduction for cell growth and MYC induction mediated by IL-2 and GM-CSF. Potentiates BMP (bone morphogenetic protein) signaling by antagonizing the inhibitory action of SMAD6 and SMAD7. Has a key role in regulation of cell surface receptor-mediated endocytosis and ubiquitin-dependent sorting of receptors to lysosomes. Endosomal localization of STAMBP is required for efficient EGFR degradation but not for its internalization (By similarity). Involved in the negative regulation of PI3K-AKT-mTOR and RAS-MAP signaling pathways.

PTMs:

Phosphorylated after BMP type I receptor activation.

Ubiquitinated by SMURF2 in the presence of RNF11.

Subcellular Location:

Nucleus. Membrane>Peripheral membrane protein. Cytoplasm. Early endosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed.

Subunit Structure:

Interacts with STAM. Interacts with SMAD6 and SMAD7. Interacts with CHMP3; the interaction appears to relieve the autoinhibition of CHMP3. Interacts with SMURF2 and RNF11; this interaction promotes ubiquitination.

Family&Domains:

The JAMM motif is essential for the protease activity.

Belongs to the peptidase M67C family.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

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