ABI2 Antibody - #DF8070

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Product: ABI2 Antibody
Catalog: DF8070
Description: Rabbit polyclonal antibody to ABI2
Application: WB IHC
Reactivity: Human, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 80 kDa; 56kD(Calculated).
Uniprot: Q9NYB9
RRID: AB_2841425

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
ABI2 Antibody detects endogenous levels of total ABI2.
RRID:
AB_2841425
Cite Format: Affinity Biosciences Cat# DF8070, RRID:AB_2841425.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Abelson interactor 2; ABI 2; Abi-2; ABI2; ABI2_HUMAN; ABI2B; Abl binding protein 3; Abl interacting protein 1 (SH3 containing protein); Abl interactor 2; Abl interactor protein 2b; Abl-binding protein 3; AblBP3; AIP 1; Arg binding protein 1; Arg protein tyrosine kinase binding protein; Arg-binding protein 1; ArgBP1; ARGBPIA; ArgBPIB; OTTHUMP00000163783; OTTHUMP00000163784; OTTHUMP00000206182; OTTHUMP00000206183; OTTHUMP00000206184; OTTHUMP00000206185; SSH3BP2;

Immunogens

Immunogen:
Uniprot:

Q9NYB9(ABI2_HUMAN) >>Visit HPA database.

Gene(ID):
ABI2(10152)
Expression:
Q9NYB9 ABI2_HUMAN:

Widely expressed. Abundant in testes, ovary, thymus, and colon, with lower but detectable levels in prostate, peripheral blood leukocytes, and spleen.

Sequence:
MAELQMLLEEEIPGGRRALFDSYTNLERVADYCENNYIQSADKQRALEETKAYTTQSLASVAYLINTLANNVLQMLDIQASQLRRMESSINHISQTVDIHKEKVARREIGILTTNKNTSRTHKIIAPANLERPVRYIRKPIDYTILDDIGHGVKWLLRFKVSTQNMKMGGLPRTTPPTQKPPSPPMSGKGTLGRHSPYRTLEPVRPPVVPNDYVPSPTRNMAPSQQSPVRTASVNQRNRTYSSSGSSGGSHPSSRSSSRENSGSGSVGVPIAVPTPSPPSVFPAPAGSAGTPPLPATSASAPAPLVPATVPSSTAPNAAAGGAPNLADGFTSPTPPVVSSTPPTGHPVQFYSMNRPASRHTPPTIGGSLPYRRPPSITSQTSLQNQMNGGPFYSQNPVSDTPPPPPPVEEPVFDESPPPPPPPEDYEEEEAAVVEYSDPYAEEDPPWAPRSYLEKVVAIYDYTKDKEDELSFQEGAIIYVIKKNDDGWYEGVMNGVTGLFPGNYVESIMHYSE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
92
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NYB9 As Substrate

Site PTM Type Enzyme
K43 Ubiquitination
S88 Phosphorylation
K123 Ubiquitination
K139 Ubiquitination
K167 Ubiquitination
T174 Phosphorylation
T175 Phosphorylation
T178 Phosphorylation
S183 Phosphorylation
S187 Phosphorylation
T191 Phosphorylation
S196 Phosphorylation
Y198 Phosphorylation
T200 Phosphorylation
R205 Methylation
Y213 Phosphorylation
S216 Phosphorylation
T218 Phosphorylation
S224 Phosphorylation
S225 Phosphorylation
S227 Phosphorylation
R230 Methylation
T231 Phosphorylation
S233 Phosphorylation
T240 Phosphorylation
Y241 Phosphorylation
S242 Phosphorylation
S244 Phosphorylation
S253 Phosphorylation
T361 Phosphorylation
T364 Phosphorylation
S368 Phosphorylation
Y371 Phosphorylation
S376 Phosphorylation
S410 Phosphorylation
Y460 Phosphorylation
T463 Phosphorylation
K466 Acetylation
K482 Acetylation
S512 Phosphorylation

Research Backgrounds

Function:

Regulator of actin cytoskeleton dynamics underlying cell motility and adhesion. Functions as a component of the WAVE complex, which activates actin nucleating machinery Arp2/3 to drive lamellipodia formation. Acts as regulator and substrate of nonreceptor tyrosine kinases ABL1 and ABL2 involved in processes linked to cell growth and differentiation. Positively regulates ABL1-mediated phosphorylation of ENAH, which is required for proper polymerization of nucleated actin filaments at the leading edge. Contributes to the regulation of actin assembly at the tips of neuron projections. In particular, controls dendritic spine morphogenesis and may promote dendritic spine specification toward large mushroom-type spines known as repositories of memory in the brain (By similarity). In hippocampal neurons, may mediate actin-dependent BDNF-NTRK2 early endocytic trafficking that triggers dendrite outgrowth (By similarity). Participates in ocular lens morphogenesis, likely by regulating lamellipodia-driven adherens junction formation at the epithelial cell-secondary lens fiber interface (By similarity). Also required for nascent adherens junction assembly in epithelial cells.

PTMs:

Phosphorylated by ABL1.

Subcellular Location:

Cytoplasm. Nucleus.

Cell projection>Lamellipodium. Cell projection>Filopodium. Cytoplasm>Cytoskeleton. Cell junction>Adherens junction.
Note: Isoform 1 but not isoform 3 is localized to protruding lamellipodia and filopodia tips (PubMed:11516653, PubMed:15572692). Present at nascent adherens junctions, where it clusters adjacent to the tips of F-actin protrusions (PubMed:15572692).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed. Abundant in testes, ovary, thymus, and colon, with lower but detectable levels in prostate, peripheral blood leukocytes, and spleen.

Subunit Structure:

Component of the WAVE complex composed of ABI2, CYFIP1 or CYFIP2, BRK1, NCKAP1 and WASF1/WAVE1. Within the complex, a heterodimer containing NCKAP1 and CYFIP1 interacts with a heterotrimer formed by WAVE1, ABI2 and BRK1. CYFIP2 binds to activated RAC1 which causes the complex to dissociate, releasing activated WASF1. Interacts (via SH3 domain) with ABL1 and ABL2.

(Microbial infection) Interacts with human cytomegalovirus UL135.

Family&Domains:

The SH3 domain is critical for binding to ABL1 and ABL2.

Belongs to the ABI family.

Research Fields

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

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