HSPA14 Antibody | Affinity Biosciences

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Western blot analysis of HSPA14 using COS7 whole cell lysates.

Product:	HSPA14 Antibody
Catalog:	DF8060
Description:	Rabbit polyclonal antibody to HSPA14
Application:	WB IF/ICC
Reactivity:	Human, Monkey
Prediction:	Pig, Bovine, Horse, Rabbit, Dog
Mol.Wt.:	55 kDa; 55kD(Calculated).
Uniprot:	Q0VDF9
RRID:	AB_2841416

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Monkey

Prediction:

Pig(100%), Bovine(80%), Horse(80%), Rabbit(91%), Dog(90%)

Clonality:

Polyclonal

Specificity:

HSPA14 Antibody detects endogenous levels of total HSPA14.

RRID:

AB_2841416
Cite Format: Affinity Biosciences Cat# DF8060, RRID:AB_2841416.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Heat shock 70 kDa protein 14; Heat shock 70kDa protein 14; Heat shock protein HSP60; Heat shock protein hsp70 related protein; HSP60; HSP70 4; HSP70 like protein 1; HSP70-like protein 1; HSP70L1; HSP7E_HUMAN; HSPA 14; HSPA14; MGC131990;

Immunogens

Immunogen:

Uniprot:

Q0VDF9(HSP7E_HUMAN) >>Visit HPA database.

Gene(ID):

HSPA14(51182)

Sequence:

Q0VDF9 HSP7E_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAIGVHLGCTSACVAVYKDGRAGVVANDAGDRVTPAVVAYSENEEIVGLAAKQSRIRNISNTVMKVKQILGRSSSDPQAQKYIAESKCLVIEKNGKLRYEIDTGEETKFVNPEDVARLIFSKMKETAHSVLGSDANDVVITVPFDFGEKQKNALGEAARAAGFNVLRLIHEPSAALLAYGIGQDSPTGKSNILVFKLGGTSLSLSVMEVNSGIYRVLSTNTDDNIGGAHFTETLAQYLASEFQRSFKHDVRGNARAMMKLTNSAEVAKHSLSTLGSANCFLDSLYEGQDFDCNVSRARFELLCSPLFNKCIEAIRGLLDQNGFTADDINKVVLCGGSSRIPKLQQLIKDLFPAVELLNSIPPDEVIPIGAAIEAGILIGKENLLVEDSLMIECSARDILVKGVDESGASRFTVLFPSGTPLPARRQHTLQAPGSISSVCLELYESDGKNSAKEETKFAQVVLQDLDKKENGLRDILAVLTMKRDGSLHVTCTDQETGKCEAISIEIAS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Rabbit

Dog

Horse

Bovine

Sheep

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q0VDF9 As Substrate

Q0VDF9

Site	PTM Type	Source
K53	Ubiquitination	Uniprot
K66	Ubiquitination	Uniprot
K68	Ubiquitination	Uniprot
S75	Phosphorylation	Uniprot
S76	Phosphorylation	Uniprot
K82	Ubiquitination	Uniprot
S87	Phosphorylation	Uniprot
K88	Ubiquitination	Uniprot
Y100	Phosphorylation	Uniprot
K109	Ubiquitination	Uniprot
K123	Ubiquitination	Uniprot
K125	Ubiquitination	Uniprot
K150	Ubiquitination	Uniprot
S186	Phosphorylation	Uniprot
S219	Phosphorylation	Uniprot
K260	Acetylation	Uniprot
Y286	Phosphorylation	Uniprot
S305	Phosphorylation	Uniprot
K310	Ubiquitination	Uniprot
K331	Ubiquitination	Uniprot
K343	Ubiquitination	Uniprot
K402	Ubiquitination	Uniprot
T420	Phosphorylation	Uniprot
T456	Phosphorylation	Uniprot
K457	Ubiquitination	Uniprot
K468	Ubiquitination	Uniprot
K469	Acetylation	Uniprot
T481	Phosphorylation	Uniprot
S487	Phosphorylation	Uniprot

Research Backgrounds

Q0VDF9_HSP7E_HUMAN

Function:

Component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, binds to the nascent polypeptide chain, while DNAJC2 stimulates its ATPase activity.

Subcellular Location:

Cytoplasm>Cytosol.

Subunit Structure:

Component of ribosome-associated complex (RAC), a heterodimer composed of Hsp70/DnaK-type chaperone HSPA14 and Hsp40/DnaJ-type chaperone DNAJC2.

Family&Domains:

Belongs to the heat shock protein 70 family.

Restrictive clause

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For Research Use Only.
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HSPA14 Antibody - #DF8060