SEMA4D Antibody | Affinity Biosciences

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Western blot analysis of extracts from Hela cells(heat-shock treatment), using SEMA4D Antibody.

Product:	SEMA4D Antibody
Catalog:	DF8058
Description:	Rabbit polyclonal antibody to SEMA4D
Application:	WB IHC
Reactivity:	Human
Prediction:	Pig, Sheep, Rabbit, Dog
Mol.Wt.:	96 kDa; 96kD(Calculated).
Uniprot:	Q92854
RRID:	AB_2841414

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(91%), Sheep(91%), Rabbit(100%), Dog(91%)

Clonality:

Polyclonal

Specificity:

SEMA4D Antibody detects endogenous levels of total SEMA4D.

RRID:

AB_2841414
Cite Format: Affinity Biosciences Cat# DF8058, RRID:AB_2841414.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

A8; BB 18; BB18; CD 100; CD100; CD100 antigen; Coll 4; COLL4; Collapsin 4; Collapsin4; GR3; Leukocyte activation antigen CD100; M sema G; MSEMA; SEM4D_HUMAN; Sema 4d; Sema domain immunoglobulin domain Ig transmembrane domain TM and short cytoplasmic domain semaphorin 4D; Sema H; SEMA J; Sema4d; Semacl 2; Semacl2; SemaH; SEMAJ; Semaphorin 4D; Semaphorin C like 2; Semaphorin H; Semaphorin J; Semaphorin-4D; Semaphorin4D; SemaphorinJ; Semcl 2; Semcl2;

Immunogens

Immunogen:

Uniprot:

Q92854(SEM4D_HUMAN) >>Visit HPA database.

Gene(ID):

SEMA4D(10507)

Expression:

Q92854 SEM4D_HUMAN:

Strongly expressed in skeletal muscle, peripheral blood lymphocytes, spleen, and thymus and also expressed at lower levels in testes, brain, kidney, small intestine, prostate, heart, placenta, lung and pancreas, but not in colon and liver.

Sequence:

Q92854 SEM4D_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MRMCTPIRGLLMALAVMFGTAMAFAPIPRITWEHREVHLVQFHEPDIYNYSALLLSEDKDTLYIGAREAVFAVNALNISEKQHEVYWKVSEDKKAKCAEKGKSKQTECLNYIRVLQPLSATSLYVCGTNAFQPACDHLNLTSFKFLGKNEDGKGRCPFDPAHSYTSVMVDGELYSGTSYNFLGSEPIISRNSSHSPLRTEYAIPWLNEPSFVFADVIRKSPDSPDGEDDRVYFFFTEVSVEYEFVFRVLIPRIARVCKGDQGGLRTLQKKWTSFLKARLICSRPDSGLVFNVLRDVFVLRSPGLKVPVFYALFTPQLNNVGLSAVCAYNLSTAEEVFSHGKYMQSTTVEQSHTKWVRYNGPVPKPRPGACIDSEARAANYTSSLNLPDKTLQFVKDHPLMDDSVTPIDNRPRLIKKDVNYTQIVVDRTQALDGTVYDVMFVSTDRGALHKAISLEHAVHIIEETQLFQDFEPVQTLLLSSKKGNRFVYAGSNSGVVQAPLAFCGKHGTCEDCVLARDPYCAWSPPTATCVALHQTESPSRGLIQEMSGDASVCPDKSKGSYRQHFFKHGGTAELKCSQKSNLARVFWKFQNGVLKAESPKYGLMGRKNLLIFNLSEGDSGVYQCLSEERVKNKTVFQVVAKHVLEVKVVPKPVVAPTLSVVQTEGSRIATKVLVASTQGSSPPTPAVQATSSGAITLPPKPAPTGTSCEPKIVINTVPQLHSEKTMYLKSSDNRLLMSLFLFFFVLFLCLFFYNCYKGYLPRQCLKFRSALLIGKKKPKSDFCDREQSLKETLVEPGSFSQQNGEHPKPALDTGYETEQDTITSKVPTDREDSQRIDDLSARDKPFDVKCELKFADSDADGD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Pig

Sheep

Dog

Horse

Bovine

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92854 As Substrate

Q92854

Site	PTM Type	Source
T5	Phosphorylation	Uniprot
N49	N-Glycosylation	Uniprot
N77	N-Glycosylation	Uniprot
K81	Ubiquitination	Uniprot
K104	Ubiquitination	Uniprot
N139	N-Glycosylation	Uniprot
K148	Ubiquitination	Uniprot
N191	N-Glycosylation	Uniprot
K269	Acetylation	Uniprot
K276	Acetylation	Uniprot
N329	N-Glycosylation	Uniprot
N379	N-Glycosylation	Uniprot
T390	Phosphorylation	Uniprot
N419	N-Glycosylation	Uniprot
K505	Ubiquitination	Uniprot
S547	Phosphorylation	Uniprot
S551	Phosphorylation	Uniprot
Y561	Phosphorylation	Uniprot
K575	Ubiquitination	Uniprot
T657	O-Glycosylation	Uniprot
T663	O-Glycosylation	Uniprot
S666	O-Glycosylation	Uniprot
T670	O-Glycosylation	Uniprot
T716	O-Glycosylation	Uniprot
S722	O-Glycosylation	Uniprot
Y727	Phosphorylation	Uniprot
S769	Phosphorylation	Uniprot
S788	Phosphorylation	Uniprot
K790	Ubiquitination	Uniprot
S798	Phosphorylation	Uniprot
K808	Ubiquitination	Uniprot
Y815	Phosphorylation	Uniprot
T817	Phosphorylation	Uniprot
K825	Ubiquitination	Uniprot
S833	Phosphorylation	Uniprot
S840	Phosphorylation	Uniprot
S857	Phosphorylation	Uniprot

Research Backgrounds

Q92854_SEM4D_HUMAN

Function:

Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling. Regulates GABAergic synapse development (By similarity). Promotes the development of inhibitory synapses in a PLXNB1-dependent manner (By similarity). Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA. Promotes the migration of cerebellar granule cells. Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro). Induces endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Tissue Specificity:

Subunit Structure:

Homodimer. Interacts with PLXNB2 (By similarity). Interacts with PLXNB1.

Family&Domains:

Belongs to the semaphorin family.

Research Fields

· Organismal Systems > Development > Axon guidance. (View pathway)

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SEMA4D Antibody - #DF8058