UNG Antibody - #DF8057

(3)  
Product: UNG Antibody
Catalog: DF8057
Description: Rabbit polyclonal antibody to UNG
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 35 kDa; 35kD(Calculated).
Uniprot: P13051
RRID: AB_2841413

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(91%), Dog(100%), Chicken(82%), Xenopus(80%)
Clonality:
Polyclonal
Specificity:
UNG Antibody detects endogenous levels of total UNG.
RRID:
AB_2841413
Cite Format: Affinity Biosciences Cat# DF8057, RRID:AB_2841413.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DGU; DKFZp781L1143; HIGM 4; HIGM4; OTTHUMP00000240527; OTTHUMP00000240528; OTTHUMP00000240529; UDG; UNG 1; UNG 15; ung; UNG_HUMAN; UNG1; UNG15; UNG2; Uracil DNA glycosylase 1; Uracil DNA glycosylase 2; Uracil DNA glycosylase; Uracil-DNA glycosylase;

Immunogens

Immunogen:
Uniprot:

P13051(UNG_HUMAN) >>Visit HPA database.

Gene(ID):
UNG(7374)
Expression:
P13051 UNG_HUMAN:

Isoform 1 is widely expressed with the highest expression in skeletal muscle, heart and testicles. Isoform 2 has the highest expression levels in tissues containing proliferating cells.

Sequence:
MIGQKTLYSFFSPSPARKRHAPSPEPAVQGTGVAGVPEESGDAAAIPAKKAPAGQEEPGTPPSSPLSAEQLDRIQRNKAAALLRLAARNVPVGFGESWKKHLSGEFGKPYFIKLMGFVAEERKHYTVYPPPHQVFTWTQMCDIKDVKVVILGQDPYHGPNQAHGLCFSVQRPVPPPPSLENIYKELSTDIEDFVHPGHGDLSGWAKQGVLLLNAVLTVRAHQANSHKERGWEQFTDAVVSWLNQNSNGLVFLLWGSYAQKKGSAIDRKRHHVLQTAHPSPLSVYRGFFGCRHFSKTNELLQKSGKKPIDWKEL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Sheep
91
Chicken
82
Xenopus
80
Rabbit
73
Zebrafish
64
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P13051 As Substrate

Site PTM Type Enzyme
K5 Acetylation
K5 Ubiquitination
T6 Phosphorylation
Y8 Phosphorylation
S9 Phosphorylation
S12 Phosphorylation P24941 (CDK2)
S14 Phosphorylation
S23 Phosphorylation
T31 Phosphorylation
K49 Ubiquitination
K50 Ubiquitination
T60 Phosphorylation
S63 Phosphorylation
S64 Phosphorylation P06493 (CDK1)
S67 Phosphorylation
K78 Ubiquitination
K100 Ubiquitination
K108 Ubiquitination
Y125 Phosphorylation
T126 Phosphorylation
Y128 Phosphorylation
T188 Phosphorylation
K261 Ubiquitination
Y284 Phosphorylation
K295 Acetylation
K295 Ubiquitination
K302 Ubiquitination

Research Backgrounds

Function:

Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.

PTMs:

Isoform 1 is processed by cleavage of a transit peptide.

Subcellular Location:

Mitochondrion.

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform 1 is widely expressed with the highest expression in skeletal muscle, heart and testicles. Isoform 2 has the highest expression levels in tissues containing proliferating cells.

Subunit Structure:

Monomer. Interacts with FAM72A.

(Microbial infection) Interacts with HIV-1 Vpr.

Family&Domains:

Belongs to the uracil-DNA glycosylase (UDG) superfamily. UNG family.

Research Fields

· Genetic Information Processing > Replication and repair > Base excision repair.

· Human Diseases > Immune diseases > Primary immunodeficiency.

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