CEACAM6 Antibody | Affinity Biosciences

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Western blot analysis of extracts from PC12 cells(heat shock treatment), using CEACAM6 Antibody.

DF8029 at 1/100 staining Rat testis tissue by IHC-P.

Product:	CEACAM6 Antibody
Catalog:	DF8029
Description:	Rabbit polyclonal antibody to CEACAM6
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	37 kDa; 37kD(Calculated).
Uniprot:	P40199
RRID:	AB_2841397

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

CEACAM6 Antibody detects endogenous levels of total CEACAM6.

RRID:

AB_2841397
Cite Format: Affinity Biosciences Cat# DF8029, RRID:AB_2841397.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Carcinoembryonic antigen related cell adhesion molecule 6; Carcinoembryonic antigen related cell adhesion molecule 6 (non specific cross reacting antigen); Carcinoembryonic antigen-related cell adhesion molecule 6; CD 66c; CD66c; CD66c antigen; CEA LIKE PROTEIN; CEACAM 6; CEACAM6; CEAL; CEAM6_HUMAN; MGC93832; NCA; Non specific cross reacting antigen; Non-specific crossreacting antigen; Normal cross reacting antigen; Normal cross-reacting antigen;

Immunogens

Immunogen:

Uniprot:

P40199(CEAM6_HUMAN) >>Visit HPA database.

Gene(ID):

CEACAM6(4680)

Expression:

P40199 CEAM6_HUMAN:

Expressed in neutrophils (PubMed:1378450). Expressed in columnar epithelial and goblet cells of the colon (PubMed:10436421). Expressed in numerous tumor cell lines (at protein level) (PubMed:16204051).

Sequence:

P40199 CEAM6_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGPPSAPPCRLHVPWKEVLLTASLLTFWNPPTTAKLTIESTPFNVAEGKEVLLLAHNLPQNRIGYSWYKGERVDGNSLIVGYVIGTQQATPGPAYSGRETIYPNASLLIQNVTQNDTGFYTLQVIKSDLVNEEATGQFHVYPELPKPSISSNNSNPVEDKDAVAFTCEPEVQNTTYLWWVNGQSLPVSPRLQLSNGNMTLTLLSVKRNDAGSYECEIQNPASANRSDPVTLNVLYGPDGPTISPSKANYRPGENLNLSCHAASNPPAQYSWFINGTFQQSTQELFIPNITVNNSGSYMCQAHNSATGLNRTTVTMITVSGSAPVLSAVATVGITIGVLARVALI

PTMs - P40199 As Substrate

P40199

Site	PTM Type	Source
N197	N-Glycosylation	Uniprot
N224	N-Glycosylation	Uniprot
S243	Phosphorylation	Uniprot
S304	Phosphorylation	Uniprot
T312	Phosphorylation	Uniprot
S326	Phosphorylation	Uniprot
T330	Phosphorylation	Uniprot

Research Backgrounds

P40199_CEAM6_HUMAN

Function:

Cell surface glycoprotein that plays a role in cell adhesion and tumor progression. Intercellular adhesion occurs in a calcium- and fibronectin-independent manner. Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM5 and CEACAM8. Heterophilic interaction with CEACAM8 occurs in activated neutrophils. Plays a role in neutrophil adhesion to cytokine-activated endothelial cells. Plays a role as an oncogene by promoting tumor progression; positively regulates cell migration, cell adhesion to endothelial cells and cell invasion. Also involved in the metastatic cascade process by inducing gain resistance to anoikis of pancreatic adenocarcinoma and colorectal carcinoma cells.

PTMs:

Glycosylated.

Subcellular Location:

Cell membrane>Lipid-anchor. Apical cell membrane. Cell surface.
Note: Localized to the apical glycocalyx surface.

Tissue Specificity:

Expressed in neutrophils. Expressed in columnar epithelial and goblet cells of the colon. Expressed in numerous tumor cell lines (at protein level).

Subunit Structure:

Homodimer; homodimerizes via its Ig-like V-type domain. Heterodimer with CEACAM8; heterodimerizes via its Ig-like V-type domain.

Family&Domains:

The extracellular N-terminus Ig-like V-type domain is necessary for homophilic and heterophilic intercellular adhesion.

Belongs to the immunoglobulin superfamily. CEA family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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CEACAM6 Antibody - #DF8029