SOX18 Antibody - #DF8018

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Product: SOX18 Antibody
Catalog: DF8018
Description: Rabbit polyclonal antibody to SOX18
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Dog
Mol.Wt.: 41 kDa; 41kD(Calculated).
Uniprot: P35713
RRID: AB_2841390

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Dog(92%)
Clonality:
Polyclonal
Specificity:
SOX18 Antibody detects endogenous levels of total SOX18.
RRID:
AB_2841390
Cite Format: Affinity Biosciences Cat# DF8018, RRID:AB_2841390.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

HLTS; SOX 18; Sox18; SOX18_HUMAN; SRY (sex determining region Y) box 18; SRY box 18; Transcription factor SOX 18; Transcription factor SOX-18;

Immunogens

Immunogen:
Uniprot:

P35713(SOX18_HUMAN) >>Visit HPA database.

Gene(ID):
SOX18(54345)
Expression:
P35713 SOX18_HUMAN:

Detected in heart, lung, placenta, skeletal muscle, liver, kidney, spleen, prostate, ovary, msosmall intestine and colon.

Sequence:
MQRSPPGYGAQDDPPARRDCAWAPGHGAAADTRGLAAGPAALAAPAAPASPPSPQRSPPRSPEPGRYGLSPAGRGERQAADESRIRRPMNAFMVWAKDERKRLAQQNPDLHNAVLSKMLGKAWKELNAAEKRPFVEEAERLRVQHLRDHPNYKYRPRRKKQARKARRLEPGLLLPGLAPPQPPPEPFPAASGSARAFRELPPLGAEFDGLGLPTPERSPLDGLEPGEAAFFPPPAAPEDCALRPFRAPYAPTELSRDPGGCYGAPLAEALRTAPPAAPLAGLYYGTLGTPGPYPGPLSPPPEAPPLESAEPLGPAADLWADVDLTEFDQYLNCSRTRPDAPGLPYHVALAKLGPRAMSCPEESSLISALSDASSAVYYSACISG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Dog
92
Chicken
58
Pig
0
Horse
0
Sheep
0
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P35713 As Substrate

Site PTM Type Enzyme
S4 Phosphorylation
Y8 Phosphorylation
S57 Phosphorylation
S70 Phosphorylation

Research Backgrounds

Function:

Transcriptional activator that binds to the consensus sequence 5'-AACAAAG-3' in the promoter of target genes and plays an essential role in embryonic cardiovascular development and lymphangiogenesis. Activates transcription of PROX1 and other genes coding for lymphatic endothelial markers. Plays an essential role in triggering the differentiation of lymph vessels, but is not required for the maintenance of differentiated lymphatic endothelial cells. Plays an important role in postnatal angiogenesis, where it is functionally redundant with SOX17. Interaction with MEF2C enhances transcriptional activation. Besides, required for normal hair development.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in heart, lung, placenta, skeletal muscle, liver, kidney, spleen, prostate, ovary, msosmall intestine and colon.

Subunit Structure:

Interacts (via C-terminus) with MEF2C (via MADS box).

Family&Domains:

Binds target DNA via the HMG box domain.

References

1). SOX18 enhances the proliferation and migration of airway smooth muscle cells induced by tumor necrosis factor-α via the regulation of Notch1 signaling. International Immunopharmacology, 2021 (PubMed: 34004439) [IF=5.6]

Application: WB    Species: mouse    Sample: airway smooth muscle cells

Fig. 2. |Knockdown of SOX18 impeded the proliferation and migration of airway smooth muscle cells induced by tumor necrosis factor-α (TNF-α). Airway smooth muscle cells were transfected with scrambled short hairpin RNA (shRNA) or SOX18 shRNA constructs and cultivated for 48 h prior to TNF-α stimulation for another 24 h.(B, C) Levels of the SOX18 protein were determined via western blotting (N = 3).
2). SOX18 exerts tumor-suppressive functions in papillary thyroid carcinoma through inhibition of Wnt/β-catenin signaling. EXPERIMENTAL CELL RESEARCH, 2020 (PubMed: 32858034) [IF=3.7]

Application: WB    Species: human    Sample: papillary thyroid carcinoma

Fig. 1.| Analysis of SOX18 expression in papillary thyroid carcinoma tissues and cell lines.(C, D)Protein expression of SOX18 in clinical specimens of papillary thyroid carcinoma was determined by Western blot. **p < 0.01.

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