Product: CALB1 Antibody
Catalog: DF8014
Description: Rabbit polyclonal antibody to CALB1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 30 kDa; 30kD(Calculated).
Uniprot: P05937
RRID: AB_2841389

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Horse(91%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
CALB1 Antibody detects endogenous levels of total CALB1.
RRID:
AB_2841389
Cite Format: Affinity Biosciences Cat# DF8014, RRID:AB_2841389.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

avian-type; CAB27; CALB 1; CALB; CALB1; CALB1_HUMAN; Calbindin 1 28kDa; Calbindin; Calbindin D28; D 28K; D-28K; D28K; OTTHUMP00000166027; OTTHUMP00000225441; RTVL H protein; Vitamin D dependent calcium binding protein; Vitamin D dependent calcium binding protein avian type; Vitamin D-dependent calcium-binding protein;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MAESHLQSSLITASQFFEIWLHFDADGSGYLEGKELQNLIQELQQARKKAGLELSPEMKTFVDQYGQRDDGKIGIVELAHVLPTEENFLLLFRCQQLKSCEEFMKTWRKYDTDHSGFIETEELKNFLKDLLEKANKTVDDTKLAEYTDLMLKLFDSNNDGKLELTEMARLLPVQENFLLKFQGIKMCGKEFNKAFELYDQDGNGYIDENELDALLKDLCEKNKQDLDINNITTYKKNIMALSDGGKLYRTDLALILCAGDN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Horse
91
Xenopus
70
Chicken
70
Pig
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P05937 As Substrate

Site PTM Type Enzyme
S55 Phosphorylation
K193 Ubiquitination
S242 Phosphorylation

Research Backgrounds

Function:

Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.

Subunit Structure:

Interacts with RANBP9.

Family&Domains:

This protein has four functional calcium-binding sites; potential sites II and VI have lost affinity for calcium.

Belongs to the calbindin family.

Research Fields

· Organismal Systems > Excretory system > Endocrine and other factor-regulated calcium reabsorption.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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