SOX10 Antibody | Affinity Biosciences

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Product:	SOX10 Antibody
Catalog:	DF8009
Description:	Rabbit polyclonal antibody to SOX10
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	60 kDa; 50kD(Calculated).
Uniprot:	P56693
RRID:	AB_2841386

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(83%)

Clonality:

Polyclonal

Specificity:

SOX10 Antibody detects endogenous levels of total SOX10.

RRID:

AB_2841386
Cite Format: Affinity Biosciences Cat# DF8009, RRID:AB_2841386.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

DOM; DOM; Dominant megacolon mouse human homolog of; MGC15649; PCWH; SOX 10; SOX10; SOX10_HUMAN; SRY (sex determining region Y) box 10; SRY (sex determining region Y) box 10; SRY box 10; SRY box containing gene 10; SRY related HMG box gene 10; SRY related HMG box gene 10; Transcription factor SOX 10; Transcription factor SOX-10; WS2E; WS4; WS4C;

Immunogens

Immunogen:

Uniprot:

P56693(SOX10_HUMAN) >>Visit HPA database.

Gene(ID):

SOX10(6663)

Expression:

P56693 SOX10_HUMAN:

Expressed in fetal brain and in adult brain, heart, small intestine and colon.

Sequence:

P56693 SOX10_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAEEQDLSEVELSPVGSEEPRCLSPGSAPSLGPDGGGGGSGLRASPGPGELGKVKKEQQDGEADDDKFPVCIREAVSQVLSGYDWTLVPMPVRVNGASKSKPHVKRPMNAFMVWAQAARRKLADQYPHLHNAELSKTLGKLWRLLNESDKRPFIEEAERLRMQHKKDHPDYKYQPRRRKNGKAAQGEAECPGGEAEQGGTAAIQAHYKSAHLDHRHPGEGSPMSDGNPEHPSGQSHGPPTPPTTPKTELQSGKADPKRDGRSMGEGGKPHIDFGNVDIGEISHEVMSNMETFDVAELDQYLPPNGHPGHVSSYSAAGYGLGSALAVASGHSAWISKPPGVALPTVSPPGVDAKAQVKTETAGPQGPPHYTDQPSTSQIAYTSLSLPHYGSAFPSISRPQFDYSDHQPSGPYYGHSGQASGLYSAFSYMGPSQRPLYTAISDPSPSGPQSHSPTHWEQPVYTTLSRP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Chicken

Xenopus

Zebrafish

Horse

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P56693 As Substrate

P56693

Site	PTM Type	Source
S8	Phosphorylation	Uniprot
S13	Phosphorylation	Uniprot
S17	Phosphorylation	Uniprot
S24	Phosphorylation	Uniprot
S27	Phosphorylation	Uniprot
S30	Phosphorylation	Uniprot
S45	Phosphorylation	Uniprot
K55	Sumoylation	Uniprot
K121	Ubiquitination	Uniprot
K136	Ubiquitination	Uniprot
K140	Acetylation	Uniprot
K140	Ubiquitination	Uniprot
Y171	Phosphorylation	Uniprot
K172	Ubiquitination	Uniprot
K208	Acetylation	Uniprot
S221	Phosphorylation	Uniprot
S224	Phosphorylation	Uniprot
S232	Phosphorylation	Uniprot
T240	Phosphorylation	Uniprot
T244	Phosphorylation	Uniprot
K246	Sumoylation	Uniprot
S346	Phosphorylation	Uniprot
K357	Sumoylation	Uniprot

Research Backgrounds

P56693_SOX10_HUMAN

Function:

Transcription factor that plays a central role in developing and mature glia. Specifically activates expression of myelin genes, during oligodendrocyte (OL) maturation, such as DUSP15 and MYRF, thereby playing a central role in oligodendrocyte maturation and CNS myelination. Once induced, MYRF cooperates with SOX10 to implement the myelination program. Transcriptional activator of MITF, acting synergistically with PAX3.

Subcellular Location:

Cytoplasm. Nucleus. Mitochondrion outer membrane>Peripheral membrane protein>Cytoplasmic side.

Tissue Specificity:

Expressed in fetal brain and in adult brain, heart, small intestine and colon.

Subunit Structure:

Monomer. Interacts with ARMCX3 at the mitochondrial outer membrane surface. Interacts with PAX3.

References

1). TGF-β-induced long non-coding RNA MIR155HG promotes the progression and EMT of laryngeal squamous cell carcinoma by regulating the miR-155-5p/SOX10 axis. INTERNATIONAL JOURNAL OF ONCOLOGY, 2019 (PubMed: 31081043) [IF=5.2]

Application: WB Species: human Sample: TU177 and AMC‑HN‑8 cells

Figure 6. |miR‑155‑5p directly targets SOX10 in LSCC cells. (A) Schematic of the potential binding sites of miR‑155‑5p on SOX10 3'UTR predicted by TargetScan and miRwark databases. (B) The relative expression of SOX10 in 45 pairs of LSCC tissues and the corresponding adjacent non‑tumor tissues by RT‑qPCR. (C) The correlation between miR‑155‑5p and SOX10 mRNA level in LSCC tissues. (D) The relative expression level of SOX10 mRNA following transfection with miR‑155‑5p mimic and inhibitor in TU177 and AMC‑HN‑8 cells by RT‑qPCR. (E) The relative protein level of SOX10 following transfection with miR‑155‑5p mimic and inhibitor in TU177 and AMC‑HN‑8 cells by western blot analysis.

2). Schwann cell‑derived exosomes induce bone marrow‑derived mesenchymal stem cells to express Schwann cell markers in vitro. Molecular Medicine Reports, 2020 (PubMed: 32016464) [IF=3.4]

Application: WB Species: rat Sample: BMSCs

Figure 3. |mRNA and protein expression levels of Schwann cell markers in differentially‑induced BMSCs. (F and G) Representative immunoblot of protein expression levels of S100, GFAP, NGRF, Sox10, EGR2 and GAPDH in control BMSCs, induced BMSCs, BMSCs + RSC96 exo, BMSCs + Fb exo or RSC96 cells. Data are presented as mean ± SD; *P<0.05, **P<0.01 and ***P<0.001. BMSCs, bone marrow mesenchymal stem cells; EGR2, early growth response 2; Fb exo, fibroblast‑derived exosomes; GFAP, glial fibrillary acidic protein; induced BMSCs, Dezawa's induction method; NGFR, low‑affinity nerve growth factor receptor; RSC96 exo, Schwann cell‑derived exosomes.

3). Effect of Exosomes from Different Cell Sources on Differentiation of Bone Marrow Mesenchymal Stem Cells into Schwann Cells.

Restrictive clause

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For Research Use Only.
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SOX10 Antibody - #DF8009