FLII Antibody - #DF8003
| Product: | FLII Antibody |
| Catalog: | DF8003 |
| Description: | Rabbit polyclonal antibody to FLII |
| Application: | WB |
| Reactivity: | Human |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Dog, Chicken, Xenopus |
| Mol.Wt.: | 145 kDa; 145kD(Calculated). |
| Uniprot: | Q13045 |
| RRID: | AB_2841382 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF8003, RRID:AB_2841382.
Fold/Unfold
Fli 1; FLI; Fli1; Flightless 1; Flightless I (Drosophila) homolog; Flightless I homolog; Flightless I homolog (Drosophila); Flightless1; FlightlessI; fliI; FLII_HUMAN; Fliih; FLIL; MGC39265; Protein flightless 1 homolog; Protein flightless-1 homolog;
Immunogens
Strongest expression in skeletal muscle with high expression also in the heart and lung.
- Q13045 FLII_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEATGVLPFVRGVDLSGNDFKGGYFPENVKAMTSLRWLKLNRTGLCYLPEELAALQKLEHLSVSHNNLTTLHGELSSLPSLRAIVARANSLKNSGVPDDIFKLDDLSVLDLSHNQLTECPRELENAKNMLVLNLSHNSIDTIPNQLFINLTDLLYLDLSENRLESLPPQMRRLVHLQTLVLNGNPLLHAQLRQLPAMTALQTLHLRSTQRTQSNLPTSLEGLSNLADVDLSCNDLTRVPECLYTLPSLRRLNLSSNQITELSLCIDQWVHVETLNLSRNQLTSLPSAICKLSKLKKLYLNSNKLDFDGLPSGIGKLTNLEEFMAANNNLELVPESLCRCPKLRKLVLNKNHLVTLPEAIHFLTEIEVLDVRENPNLVMPPKPADRAAEWYNIDFSLQNQLRLAGASPATVAAAAAAGSGPKDPMARKMRLRRRKDSAQDDQAKQVLKGMSDVAQEKNKKQEESADARAPSGKVRRWDQGLEKPRLDYSEFFTEDVGQLPGLTIWQIENFVPVLVEEAFHGKFYEADCYIVLKTFLDDSGSLNWEIYYWIGGEATLDKKACSAIHAVNLRNYLGAECRTVREEMGDESEEFLQVFDNDISYIEGGTASGFYTVEDTHYVTRMYRVYGKKNIKLEPVPLKGTSLDPRFVFLLDRGLDIYVWRGAQATLSSTTKARLFAEKINKNERKGKAEITLLVQGQELPEFWEALGGEPSEIKKHVPEDFWPPQPKLYKVGLGLGYLELPQINYKLSVEHKQRPKVELMPRMRLLQSLLDTRCVYILDCWSDVFIWLGRKSPRLVRAAALKLGQELCGMLHRPRHATVSRSLEGTEAQVFKAKFKNWDDVLTVDYTRNAEAVLQSPGLSGKVKRDAEKKDQMKADLTALFLPRQPPMSLAEAEQLMEEWNEDLDGMEGFVLEGKKFARLPEEEFGHFYTQDCYVFLCRYWVPVEYEEEEKKEDKEEKAEGKEGEEATAEAEEKQPEEDFQCIVYFWQGREASNMGWLTFTFSLQKKFESLFPGKLEVVRMTQQQENPKFLSHFKRKFIIHRGKRKAVQGAQQPSLYQIRTNGSALCTRCIQINTDSSLLNSEFCFILKVPFESEDNQGIVYAWVGRASDPDEAKLAEDILNTMFDTSYSKQVINEGEEPENFFWVGIGAQKPYDDDAEYMKHTRLFRCSNEKGYFAVTEKCSDFCQDDLADDDIMLLDNGQEVYMWVGTQTSQVEIKLSLKACQVYIQHMRSKEHERPRRLRLVRKGNEQHAFTRCFHAWSAFCKALA
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q13045 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| T4 | Phosphorylation | Uniprot | |
| K21 | Acetylation | Uniprot | |
| K21 | Ubiquitination | Uniprot | |
| K39 | Ubiquitination | Uniprot | |
| S64 | Phosphorylation | Uniprot | |
| K92 | Ubiquitination | Uniprot | |
| K102 | Ubiquitination | Uniprot | |
| K296 | Ubiquitination | Uniprot | |
| K303 | Ubiquitination | Uniprot | |
| K315 | Ubiquitination | Uniprot | |
| K344 | Ubiquitination | Uniprot | |
| K381 | Ubiquitination | Uniprot | |
| Y390 | Phosphorylation | Uniprot | |
| S406 | Phosphorylation | Uniprot | |
| T409 | Phosphorylation | Uniprot | |
| S418 | Phosphorylation | Uniprot | |
| K421 | Ubiquitination | Uniprot | |
| S436 | Phosphorylation | Q96BR1 (SGK3) | Uniprot |
| K447 | Ubiquitination | Uniprot | |
| S463 | Phosphorylation | Uniprot | |
| K472 | Ubiquitination | Uniprot | |
| K482 | Ubiquitination | Uniprot | |
| Y523 | Phosphorylation | Uniprot | |
| Y528 | Phosphorylation | Uniprot | |
| K631 | Ubiquitination | Uniprot | |
| K638 | Ubiquitination | Uniprot | |
| Y657 | Phosphorylation | Uniprot | |
| K671 | Ubiquitination | Uniprot | |
| K678 | Ubiquitination | Uniprot | |
| K715 | Ubiquitination | Uniprot | |
| K730 | Ubiquitination | Uniprot | |
| Y737 | Phosphorylation | Uniprot | |
| K746 | Ubiquitination | Uniprot | |
| K752 | Ubiquitination | Uniprot | |
| K756 | Ubiquitination | Uniprot | |
| S768 | Phosphorylation | Uniprot | |
| T772 | Phosphorylation | Uniprot | |
| S792 | Phosphorylation | Uniprot | |
| K802 | Ubiquitination | Uniprot | |
| T818 | Phosphorylation | Q96BR1 (SGK3) | Uniprot |
| S822 | Phosphorylation | Uniprot | |
| K832 | Methylation | Uniprot | |
| K832 | Ubiquitination | Uniprot | |
| K836 | Ubiquitination | Uniprot | |
| S856 | Phosphorylation | Uniprot | |
| S860 | Phosphorylation | Uniprot | |
| K862 | Ubiquitination | Uniprot | |
| K874 | Ubiquitination | Uniprot | |
| Y946 | Phosphorylation | Uniprot | |
| K951 | Ubiquitination | Uniprot | |
| K962 | Ubiquitination | Uniprot | |
| T968 | Phosphorylation | Uniprot | |
| K1007 | Ubiquitination | Uniprot | |
| K1015 | Ubiquitination | Uniprot | |
| K1029 | Ubiquitination | Uniprot | |
| K1035 | Ubiquitination | Uniprot | |
| K1046 | Ubiquitination | Uniprot | |
| Y1102 | Phosphorylation | Uniprot | |
| K1115 | Ubiquitination | Uniprot | |
| K1162 | Ubiquitination | Uniprot | |
| K1173 | Ubiquitination | Uniprot | |
| Y1175 | Phosphorylation | Uniprot | |
| Y1227 | Phosphorylation | Uniprot |
Research Backgrounds
May play a role as coactivator in transcriptional activation by hormone-activated nuclear receptors (NR) and acts in cooperation with NCOA2 and CARM1. Involved in estrogen hormone signaling. Involved in early embryonic development (By similarity). May play a role in regulation of cytoskeletal rearrangements involved in cytokinesis and cell migration, by inhibiting Rac1-dependent paxillin phosphorylation.
Nucleus. Cytoplasm>Cytoskeleton. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cell junction>Focal adhesion.
Note: Colocalizes to actin-rich structures in blastocysts and, together with HRAS, RHOA and CDC42, in migrating fibroblasts. Localizes to centrosomes (By similarity).
Strongest expression in skeletal muscle with high expression also in the heart and lung.
Interacts with actin, ACTL6A, NCOA2, CARM1 and MYD88. Interacts with LRRFIP1 and LRRFIP2. Upon LPS stimulation, LRRFIP2 competes for MYD88-binding. LRRFIP1 constitutively blocks the interaction with MyD88, even in the absence of LPS. Interacts with the nuclear receptors ESR1 and THRB. Interacts with SGK3.
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