FSCN1 Antibody | Affinity Biosciences

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Western blot analysis of FSCN1 using Jurkat whole lysates.

DF7988 at 1/100 staining Mouse brain tissue by IHC-P.

Product:	FSCN1 Antibody
Catalog:	DF7988
Description:	Rabbit polyclonal antibody to FSCN1
Application:	WB IHC
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Sheep, Dog, Chicken
Mol.Wt.:	54 kDa; 55kD(Calculated).
Uniprot:	Q16658
RRID:	AB_2841372

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Bovine(100%), Horse(88%), Sheep(100%), Dog(100%), Chicken(100%)

Clonality:

Polyclonal

Specificity:

FSCN1 Antibody detects endogenous levels of total FSCN1.

RRID:

AB_2841372
Cite Format: Affinity Biosciences Cat# DF7988, RRID:AB_2841372.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

55 kDa actin bundling protein; 55 kDa actin-bundling protein; Actin bundling protein; actin bundling protein, 55-KD; FAN 1; FAN1; Fascin 1; Fascin actin bundling protein 1; Fascin; Fascin homolog 1 actin bundling protein (Strongylocentrotus purpuratus); Fascin homolog 1; Fascin, sea urchin, homolog of, 1; Fascin1; FLJ38511; FSCN 1; FSCN1; FSCN1_HUMAN; HSN; p55; Singed (Drosophila) like (sea urchin fascin homolog like); Singed drosophila homolog like; Singed like (fascin homolog sea urchin) (Drosophila); Singed like (fascin homolog sea urchin); Singed like protein; Singed, drosophila, homolog of; Singed-like protein; SNL; Strongylocentrotus purpuratus;

Immunogens

Immunogen:

Uniprot:

Q16658(FSCN1_HUMAN) >>Visit HPA database.

Gene(ID):

FSCN1(6624)

Expression:

Q16658 FSCN1_HUMAN:

Ubiquitous.

Sequence:

Q16658 FSCN1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MTANGTAEAVQIQFGLINCGNKYLTAEAFGFKVNASASSLKKKQIWTLEQPPDEAGSAAVCLRSHLGRYLAADKDGNVTCEREVPGPDCRFLIVAHDDGRWSLQSEAHRRYFGGTEDRLSCFAQTVSPAEKWSVHIAMHPQVNIYSVTRKRYAHLSARPADEIAVDRDVPWGVDSLITLAFQDQRYSVQTADHRFLRHDGRLVARPEPATGYTLEFRSGKVAFRDCEGRYLAPSGPSGTLKAGKATKVGKDELFALEQSCAQVVLQAANERNVSTRQGMDLSANQDEETDQETFQLEIDRDTKKCAFRTHTGKYWTLTATGGVQSTASSKNASCYFDIEWRDRRITLRASNGKFVTSKKNGQLAASVETAGDSELFLMKLINRPIIVFRGEHGFIGCRKVTGTLDANRSSYDVFQLEFNDGAYNIKDSTGKYWTVGSDSAVTSSGDTPVDFFFEFCDYNKVAIKVGGRYLKGDHAGVLKASAETVDPASLWEY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Dog

100

Chicken

100

Horse

Xenopus

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q16658 As Substrate

Q16658

Site	PTM Type	Enzyme	Source
T2	Acetylation		Uniprot
Y23	Phosphorylation		Uniprot
S38	Phosphorylation		Uniprot
S39	Phosphorylation	P17252 (PRKCA) , Q05655 (PRKCD)	Uniprot
K41	Acetylation		Uniprot
K41	Ubiquitination		Uniprot
K42	Ubiquitination		Uniprot
K43	Acetylation		Uniprot
K43	Ubiquitination		Uniprot
Y69	Phosphorylation		Uniprot
K74	Acetylation		Uniprot
K74	Ubiquitination		Uniprot
T79	Phosphorylation		Uniprot
C80	S-Nitrosylation		Uniprot
R118	Methylation		Uniprot
S120	Phosphorylation		Uniprot
S127	Phosphorylation		Uniprot
Y145	Phosphorylation		Uniprot
S146	Phosphorylation		Uniprot
T148	Phosphorylation		Uniprot
R217	Methylation		Uniprot
S218	Phosphorylation		Uniprot
S234	Phosphorylation		Uniprot
S237	Phosphorylation		Uniprot
T239	Phosphorylation		Uniprot
K241	Ubiquitination		Uniprot
K247	Ubiquitination		Uniprot
K250	Acetylation		Uniprot
K250	Ubiquitination		Uniprot
S274	Phosphorylation		Uniprot
K304	Acetylation		Uniprot
K313	Ubiquitination		Uniprot
Y314	Phosphorylation		Uniprot
S333	Phosphorylation		Uniprot
K353	Methylation		Uniprot
K353	Ubiquitination		Uniprot
K359	Ubiquitination		Uniprot
C397	S-Nitrosylation		Uniprot
K399	Sumoylation		Uniprot
K399	Ubiquitination		Uniprot
T403	Phosphorylation		Uniprot
Y411	Phosphorylation		Uniprot
K426	Ubiquitination		Uniprot
Y432	Phosphorylation		Uniprot
T447	Phosphorylation		Uniprot
Y458	Phosphorylation		Uniprot
K464	Ubiquitination		Uniprot
K471	Acetylation		Uniprot
K471	Ubiquitination		Uniprot
K479	Ubiquitination		Uniprot
T484	Phosphorylation		Uniprot

Research Backgrounds

Q16658_FSCN1_HUMAN

Function:

Actin-binding protein that contains 2 major actin binding sites. Organizes filamentous actin into parallel bundles. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration. Mediates reorganization of the actin cytoskeleton and axon growth cone collapse in response to NGF.

PTMs:

Phosphorylation at Ser-39 inhibits actin-binding. Phosphorylation is required for the reorganization of the actin cytoskeleton in response to NGF.

Subcellular Location:

Cytoplasm>Cytosol. Cytoplasm>Cell cortex. Cytoplasm>Cytoskeleton. Cytoplasm>Cytoskeleton>Stress fiber. Cell projection>Filopodium. Cell projection>Invadopodium. Cell projection>Microvillus. Cell junction.
Note: Colocalized with RUFY3 and F-actin at filipodia of the axonal growth cone. Colocalized with DBN1 and F-actin at the transitional domain of the axonal growth cone (By similarity).

Tissue Specificity:

Ubiquitous.

Subunit Structure:

Interacts with RUFY3 (via N-terminus); the interaction induces neuron axon development (By similarity). Interacts with NGFR (By similarity). Associates with CTNNB1 (By similarity). Interacts with PLXNB3.

Family&Domains:

Composed of four fascin beta-trefoil domains.

Belongs to the fascin family.

Research Fields

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

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FSCN1 Antibody - #DF7988