MBD1 Antibody - #DF7981

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Product: MBD1 Antibody
Catalog: DF7981
Description: Rabbit polyclonal antibody to MBD1
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 67 kDa; 67kD(Calculated).
Uniprot: Q9UIS9
RRID: AB_2841368

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Horse(100%), Sheep(90%), Rabbit(90%), Dog(100%)
Clonality:
Polyclonal
Specificity:
MBD1 Antibody detects endogenous levels of total MBD1.
RRID:
AB_2841368
Cite Format: Affinity Biosciences Cat# DF7981, RRID:AB_2841368.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CXXC 3; CXXC-type zinc finger protein 3; CXXC3; MBD 1; Mbd1; MBD1_HUMAN; MECP1 COMPLEX; Methyl CpG binding domain protein 1; Methyl CpG binding domain protein 1 isoform PCM1; Methyl CpG binding protein MBD1; Methyl CpG binding protein splice variant 1; Methyl CpG binding protein splice variant 2; Methyl CpG binding protein splice variant 3; Methyl CpG binding protein splice variant 4; Methyl-CpG-binding domain protein 1; Methyl-CpG-binding protein MBD1; PCM 1; PCM1; Protein containing methyl-CpG-binding domain 1; Regulator of fibroblast growth factor 2 (FGF 2) transcription; RFT; The regulator of fibroblast growth factor 2(FGF 2) transcription;

Immunogens

Immunogen:
Uniprot:

Q9UIS9(MBD1_HUMAN) >>Visit HPA database.

Gene(ID):
MBD1(4152)
Expression:
Q9UIS9 MBD1_HUMAN:

Widely expressed.

Sequence:
MAEDWLDCPALGPGWKRREVFRKSGATCGRSDTYYQSPTGDRIRSKVELTRYLGPACDLTLFDFKQGILCYPAPKAHPVAVASKKRKKPSRPAKTRKRQVGPQSGEVRKEAPRDETKADTDTAPASFPAPGCCENCGISFSGDGTQRQRLKTLCKDCRAQRIAFNREQRMFKRVGCGECAACQVTEDCGACSTCLLQLPHDVASGLFCKCERRRCLRIVERSRGCGVCRGCQTQEDCGHCPICLRPPRPGLRRQWKCVQRRCLRGKHARRKGGCDSKMAARRRPGAQPLPPPPPSQSPEPTEPHPRALAPSPPAEFIYYCVDEDELQPYTNRRQNRKCGACAACLRRMDCGRCDFCCDKPKFGGSNQKRQKCRWRQCLQFAMKRLLPSVWSESEDGAGSPPPYRRRKRPSSARRHHLGPTLKPTLATRTAQPDHTQAPTKQEAGGGFVLPPPGTDLVFLREGASSPVQVPGPVAASTEALLQEAQCSGLSWVVALPQVKQEKADTQDEWTPGTAVLTSPVLVPGCPSKAVDPGLPSVKQEPPDPEEDKEENKDDSASKLAPEEEAGGAGTPVITEIFSLGGTRFRDTAVWLPRSKDLKKPGARKQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Sheep
90
Rabbit
90
Bovine
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UIS9 As Substrate

Site PTM Type Enzyme
S37 Phosphorylation
K46 Sumoylation
K46 Ubiquitination
Y52 Phosphorylation
K75 Ubiquitination
K84 Ubiquitination
K109 Ubiquitination
T116 Phosphorylation
T120 Phosphorylation
K277 Sumoylation
K277 Ubiquitination
S295 Phosphorylation
S297 Phosphorylation
T301 Phosphorylation
S311 Phosphorylation
K337 Ubiquitination
S365 Phosphorylation
K383 Ubiquitination
S388 Phosphorylation
S391 Phosphorylation
S393 Phosphorylation
S399 Phosphorylation
Y403 Phosphorylation
K422 Acetylation
K422 Sumoylation
K422 Ubiquitination
S464 Phosphorylation
K499 Sumoylation
K502 Sumoylation
K502 Ubiquitination
T505 Phosphorylation
K538 Sumoylation
S557 Phosphorylation
K558 Ubiquitination
T570 Phosphorylation
T574 Phosphorylation
T587 Phosphorylation
R593 Methylation
K595 Methylation

Research Backgrounds

Function:

Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters.

PTMs:

Sumoylated with SUMO1 by PIAS1 and PIAS3. Sumoylation affects transcriptional silencing by preventing the interaction with SETDB1. In contrast, sumoylation may increase interaction with AFT7IP.

Subcellular Location:

Nucleus. Nucleus matrix. Nucleus speckle. Chromosome.
Note: Colocalizes with the Ten-1 ICD form of TENM1 in foci associated with the nuclear matrix (By similarity). Nuclear, in a punctate pattern. Associated with euchromatic regions of the chromosomes, with pericentromeric regions on chromosome 1 and with telomeric regions from several chromosomes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed.

Subunit Structure:

Interacts with the Ten-1 ICD form of TENM1 (By similarity). Interacts with OASL, AFT7IP, AFT7IP2 and BAHD1. Binds CHAF1A and the SUV39H1-CBX5 complex via the MBD domain. Binds MGP via the TRD domain. May be part of the MeCP1 complex. During DNA replication, it recruits SETDB1 to form a S phase-specific complex that facilitates methylation of H3 'Lys-9' during replication-coupled chromatin assembly and is at least composed of the CAF-1 subunit CHAF1A, MBD1 and SETDB1.

Family&Domains:

The methyl-CpG-binding domain (MBD) functions both in binding to methylated DNA and in protein interactions.

The third CXXC-type zinc finger mediates binding to DNA containing unmethylated CpG dinucleotides.

The transcriptional repression domain (TRD) is involved in transcription repression and in protein interactions.

Restrictive clause

 

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