RGS9 Antibody | Affinity Biosciences

1/1

Western blot analysis of RGS9 using K562 whole lysates.

Product:	RGS9 Antibody
Catalog:	DF7979
Description:	Rabbit polyclonal antibody to RGS9
Application:	WB
Reactivity:	Human, Mouse
Prediction:	Pig
Mol.Wt.:	55 kDa; 77kD(Calculated).
Uniprot:	O75916
RRID:	AB_2841366

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(88%)

Clonality:

Polyclonal

Specificity:

RGS9 Antibody detects endogenous levels of total RGS9.

RRID:

AB_2841366
Cite Format: Affinity Biosciences Cat# DF7979, RRID:AB_2841366.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

PERRS; R9AP; Regulator of G protein signalling 9; Regulator of G-protein signaling 9; RGS9; RGS9_HUMAN; RGS9L;

Immunogens

Immunogen:

Uniprot:

O75916(RGS9_HUMAN) >>Visit HPA database.

Gene(ID):

RGS9(8787)

Expression:

O75916 RGS9_HUMAN:

Highly expressed in the caudate and putamen, lower levels found in the hypothalamus and nucleus accumbens and very low levels in cerebellum. Not expressed in globus pallidus or cingulate cortex. Isoform 2 is expressed predominantly in pineal gland and retina. Isoform 3 is expressed in retina (abundant in photoreceptors).

Sequence:

O75916 RGS9_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MTIRHQGQQYRPRMAFLQKIEALVKDMQNPETGVRMQNQRVLVTSVPHAMTGSDVLQWIVQRLWISSLEAQNLGNFIVRYGYIYPLQDPKNLILKPDGSLYRFQTPYFWPTQQWPAEDTDYAIYLAKRNIKKKGILEEYEKENYNFLNQKMNYKWDFVIMQAKEQYRAGKERNKADRYALDCQEKAYWLVHRCPPGMDNVLDYGLDRVTNPNEVKVNQKQTVVAVKKEIMYYQQALMRSTVKSSVSLGGIVKYSEQFSSNDAIMSGCLPSNPWITDDTQFWDLNAKLVEIPTKMRVERWAFNFSELIRDPKGRQSFQYFLKKEFSGENLGFWEACEDLKYGDQSKVKEKAEEIYKLFLAPGARRWINIDGKTMDITVKGLKHPHRYVLDAAQTHIYMLMKKDSYARYLKSPIYKDMLAKAIEPQETTKKSSTLPFMRRHLRSSPSPVILRQLEEEAKAREAANTVDITQPGQHMAPSPHLTVYTGTCMPPSPSSPFSSSCRSPRKPFASPSRFIRRPSTTICPSPIRVALESSSGLEQKGECSGSMAPRGPSVTESSEASLDTSWPRSRPRAPPKARMALSFSRFLRRGCLASPVFARLSPKCPAVSHGRVQPLGDVGQQLPRLKSKRVANFFQIKMDVPTGSGTCLMDSEDAGTGESGDRATEKEVICPWESL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Dog

Bovine

Sheep

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75916 As Substrate

O75916

Site	PTM Type	Source
S66	Phosphorylation	Uniprot
K141	Acetylation	Uniprot
K141	Ubiquitination	Uniprot
Y153	Phosphorylation	Uniprot
K185	Ubiquitination	Uniprot
S304	Phosphorylation	Uniprot
K409	Ubiquitination	Uniprot
K419	Acetylation	Uniprot
S445	Phosphorylation	Uniprot
S545	Phosphorylation	Uniprot
S556	Phosphorylation	Uniprot
T563	Phosphorylation	Uniprot
S564	Phosphorylation	Uniprot
S568	Phosphorylation	Uniprot

Research Backgrounds

O75916_RGS9_HUMAN

Function:

Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to GNAT1. Involved in phototransduction; key element in the recovery phase of visual transduction (By similarity).

PTMs:

Retinal isoform 3 is light-dependent phosphorylated at 'Ser-478'. Phosphorylation is decreased by light exposition (By similarity).

Subcellular Location:

Membrane>Peripheral membrane protein.
Note: Isoform 3 is targeted to the membrane via its interaction with RGS9BP.

Tissue Specificity:

Subunit Structure:

Heterodimer with GNB5. Interacts with RGS7BP, leading to regulate the subcellular location of the heterodimer formed with GNB5. Component of the RGS9-1-Gbeta5 complex composed of RGS9 (RGS9-1), Gbeta5 (GNB5) and RGS9BP. Interacts with PDE6G and GNAT1.

Family&Domains:

In photoreceptor cells the DEP domain is essential for targeting RGS9 to the outer rod segments.

Research Fields

· Human Diseases > Substance dependence > Cocaine addiction.

· Organismal Systems > Sensory system > Phototransduction.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

RGS9 Antibody - #DF7979