MESDC2 Antibody | Affinity Biosciences

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Western blot analysis of MESDC2 using NIH-3T3 whole lysates.

DF7971 at 1/100 staining Human prostate cancer by IHC-P.

DF7971 at 1/100 staining Mouse testis tissue by IHC-P.

DF7971 at 1/100 staining Rat heart tissue by IHC-P.

Product:	MESDC2 Antibody
Catalog:	DF7971
Description:	Rabbit polyclonal antibody to MESDC2
Application:	WB IHC
Reactivity:	Human, Mouse
Prediction:	Zebrafish, Bovine, Chicken
Mol.Wt.:	26 kDa; 26kD(Calculated).
Uniprot:	Q14696
RRID:	AB_2841361

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Zebrafish(100%), Bovine(100%), Chicken(100%)

Clonality:

Polyclonal

Specificity:

MESDC2 Antibody detects endogenous levels of total MESDC2.

RRID:

AB_2841361
Cite Format: Affinity Biosciences Cat# DF7971, RRID:AB_2841361.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

BOCA; KIAA0081; LDLR chaperone MESD; MESD; MESD_HUMAN; MESDC 2; mesdc2; Mesoderm development candidate 2; Mesoderm development protein; Renal carcinoma antigen NY REN 61; Renal carcinoma antigen NY-REN-61;

Immunogens

Immunogen:

Uniprot:

Q14696(MESD_HUMAN) >>Visit HPA database.

Gene(ID):

MESDC2(23184)

Sequence:

Q14696 MESD_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAASRWARKAVVLLCASDLLLLLLLLPPPGSCAAEGSPGTPDESTPPPRKKKKDIRDYNDADMARLLEQWEKDDDIEEGDLPEHKRPSAPVDFSKIDPSKPESILKMTKKGKTLMMFVTVSGSPTEKETEEITSLWQGSLFNANYDVQRFIVGSDRAIFMLRDGSYAWEIKDFLVGQDRCADVTLEGQVYPGKGGGSKEKNKTKQDKGKKKKEGDLKSRSSKEENRAGNKREDL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Zebrafish

100

Bovine

100

Chicken

100

Pig

Horse

Sheep

Dog

Xenopus

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14696 As Substrate

Q14696

Site	PTM Type	Source
Y58	Phosphorylation	Uniprot
K72	Ubiquitination	Uniprot
K85	Ubiquitination	Uniprot
S88	O-Glycosylation	Uniprot
S88	Phosphorylation	Uniprot
K95	Methylation	Uniprot
T113	Phosphorylation	Uniprot
S121	Phosphorylation	Uniprot
S123	Phosphorylation	Uniprot
T125	Phosphorylation	Uniprot
R179	Methylation	Uniprot
K207	Ubiquitination	Uniprot
K209	Ubiquitination	Uniprot
K210	Ubiquitination	Uniprot
K211	Ubiquitination	Uniprot

Research Backgrounds

Q14696_MESD_HUMAN

Function:

Chaperone specifically assisting the folding of beta-propeller/EGF modules within the family of low-density lipoprotein receptors (LDLRs). Acts as a modulator of the Wnt pathway through chaperoning the coreceptors of the canonical Wnt pathway, LRP5 and LRP6, to the plasma membrane. Essential for specification of embryonic polarity and mesoderm induction. Plays an essential role in neuromuscular junction (NMJ) formation by promoting cell-surface expression of LRP4 (By similarity). May regulate phagocytosis of apoptotic retinal pigment epithelium (RPE) cells (By similarity).

Subcellular Location:

Endoplasmic reticulum.
Note: Released from apoptotic cells and shed photoreceptor outer segments.

Subunit Structure:

Monomer. Interacts with LRP5; the interaction prevents LRP5 from forming aggregates and chaperones LRP6 to the plasma membrane. Interacts with LRP6; the interaction prevents LRP6 from forming aggregates and chaperones LRP6 to the plasma membrane. Interacts with LRP4; the interaction promotes glycosylation of LRP4 and its cell-surface expression (By similarity).

Family&Domains:

The chaperone domain provides a folding template for proper folding of the beta-propeller (BP) domains of LRP5/6.

The escort domain ensures LRP5/6 safe-trafficking from the ER to the Golgi by preventing premature ligand-binding.

Belongs to the MESD family.

Restrictive clause

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For Research Use Only.
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MESDC2 Antibody - #DF7971