USP18 Antibody - #DF7968

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Product: USP18 Antibody
Catalog: DF7968
Description: Rabbit polyclonal antibody to USP18
Application: WB
Reactivity: Human
Mol.Wt.: 43 kDa.; 43kD(Calculated).
Uniprot: Q9UMW8
RRID: AB_2841360

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
USP18 Antibody detects endogenous levels of total USP18.
RRID:
AB_2841360
Cite Format: Affinity Biosciences Cat# DF7968, RRID:AB_2841360.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

43 kDa ISG15 specific protease; 43 kDa ISG15-specific protease; EC 3.1.2.; hUBP43; Interferon Stimulated Gene 43 kD; ISG15 Specific Processing Protease; ISG15-specific-processing protease; ISG43; Ubiquitin Specific Peptidase 18; Ubiquitin Specific Protease 18 43 kD; Ubiquitin Specific Protease 18; Ubl carboxyl terminal hydrolase 18; Ubl carboxyl-terminal hydrolase 18; Ubl thioesterase 18; Ubl thiolesterase 18; Ubp15; UBP18_HUMAN; USP18;

Immunogens

Immunogen:
Uniprot:

Q9UMW8(UBP18_HUMAN) >>Visit HPA database.

Gene(ID):
USP18(11274)
Sequence:
MSKAFGLLRQICQSILAESSQSPADLEEKKEEDSNMKREQPRERPRAWDYPHGLVGLHNIGQTCCLNSLIQVFVMNVDFTRILKRITVPRGADEQRRSVPFQMLLLLEKMQDSRQKAVRPLELAYCLQKCNVPLFVQHDAAQLYLKLWNLIKDQITDVHLVERLQALYTIRVKDSLICVDCAMESSRNSSMLTLPLSLFDVDSKPLKTLEDALHCFFQPRELSSKSKCFCENCGKKTRGKQVLKLTHLPQTLTIHLMRFSIRNSQTRKICHSLYFPQSLDFSQILPMKRESCDAEEQSGGQYELFAVIAHVGMADSGHYCVYIRNAVDGKWFCFNDSNICLVSWEDIQCTYGNPNYHWQETAYLLVYMKMEC

PTMs - Q9UMW8 As Substrate

Site PTM Type Enzyme
K3 Ubiquitination
Y125 Phosphorylation
K173 Ubiquitination
K268 Ubiquitination
K288 Ubiquitination

Research Backgrounds

Function:

Involved in the regulation of inflammatory response to interferon type 1. Can efficiently cleave only ISG15 fusions including native ISG15 conjugates linked via isopeptide bonds. Necessary to maintain a critical cellular balance of ISG15-conjugated proteins in both healthy and stressed organisms.

Has enzymatic activity similar to isoform 1 and interferes with type I interferon signaling. Major deISGylation enzyme for nuclear proteins.

Subcellular Location:

Cytoplasm.

Nucleus. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the peptidase C19 family.

References

1). USP18‑deficiency in cervical carcinoma is crucial for the malignant behavior of tumor cells in an ERK signal‑dependent manner. Oncology Letters, 2021 (PubMed: 33850562) [IF=2.9]

Application: IHC    Species: Human    Sample: tumor tissues

Figure 1. Downregulated USP18 expression in tumor tissues of a cervix tissue microarray. UCC tissues with grade (A) 1 and (B) 2 exhibited moderate staining, and grade (C) 3 exhibited weak staining of USP18. (D) Non-cancer tissues exhibited super intensive staining of USP18. Magnification, ×200. USP18, ubiquitin-specific peptidase 18.

Application: WB    Species: Human    Sample: HeLa cells

Figure 2. USP18 expression in HeLa cells. (A) Representative images of USP18 protein expression from western blot assay. (B) Fluorescence images of mock- and USP18-siRNA-transfected HeLa cells. Magnification, ×200. (C) Statistical analysis of USP18 protein expression. **P<0.01 vs. si-USP18. USP18, ubiquitin-specific peptidase 18; siRNA, small interfering RNA.

Restrictive clause

 

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For Research Use Only.
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