beta Tubulin Antibody | Affinity Biosciences

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FIGURE 8 H9c2 cells were treated with 1 μM AngII, (0.

Product:	beta Tubulin Antibody
Catalog:	DF7967
Description:	Rabbit polyclonal antibody to beta Tubulin
Application:	WB
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	50 kDa; 50kD(Calculated).
Uniprot:	P07437
RRID:	AB_2841359

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

beta Tubulin Antibody detects endogenous levels of total beta Tubulin.

RRID:

AB_2841359
Cite Format: Affinity Biosciences Cat# DF7967, RRID:AB_2841359.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Tubulin beta 2b; Alpha tubulin; Alpha-tubulin ubiquitous; beta Ib tubulin; CDCBM5; CDCBM6; fd02b12; K ALPHA 1; M40; OK/SW-cl.56; TBA1B_HUMAN; TUBA1B; TUBB1; TUBB2; TUBB5; tubulin alpha 1b; Tubulin alpha-1B chain; Tubulin alpha-ubiquitous chain; Tubulin beta 1b; Tubulin beta 2A; tubulin beta 2A class IIa; Tubulin beta; tubulin beta chain; tubulin beta class I; tubulin beta-1 chain; tubulin beta-2A chain; tubulin beta-5 chain; Tubulin K-alpha-1; tubulin, alpha, ubiquitous; tubulin, beta 2A class IIa; tubulin, beta polypeptide 2; tubulin, beta polypeptide; tubulin, beta, class IIA; wu:fd02b12; zgc:55461;

Immunogens

Immunogen:

A synthesized peptide derived from human beta Tubulin, corresponding to a region within C-terminal amino acids.

Uniprot:

P07437(TBB5_HUMAN) >>Visit HPA database.

Gene(ID):

TUBB(203068)

Expression:

P07437 TBB5_HUMAN:

Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

Sequence:

P07437 TBB5_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATAEEEEDFGEEAEEEA

PTMs - P07437 As Substrate

P07437

Site	PTM Type	Enzyme	Source
R2	Methylation		Uniprot
C12	S-Nitrosylation		Uniprot
K19	Acetylation		Uniprot
K19	Methylation		Uniprot
K19	Ubiquitination		Uniprot
S25	Phosphorylation		Uniprot
T33	Phosphorylation		Uniprot
T35	Phosphorylation		Uniprot
Y36	Phosphorylation		Uniprot
S40	Phosphorylation		Uniprot
R46	Methylation		Uniprot
S48	Phosphorylation		Uniprot
Y50	Phosphorylation		Uniprot
Y51	Phosphorylation		Uniprot
T55	Phosphorylation		Uniprot
K58	Acetylation		Uniprot
K58	Methylation		Uniprot
K58	Sumoylation		Uniprot
K58	Ubiquitination		Uniprot
Y59	Phosphorylation		Uniprot
T72	Phosphorylation		Uniprot
S75	Phosphorylation		Uniprot
S78	Phosphorylation		Uniprot
S95	Phosphorylation		Uniprot
K103	Acetylation		Uniprot
K103	Sumoylation		Uniprot
K103	Ubiquitination		Uniprot
Y106	Phosphorylation		Uniprot
T107	Phosphorylation		Uniprot
S115	Phosphorylation		Uniprot
K122	Ubiquitination		Uniprot
S126	Phosphorylation		Uniprot
T136	Phosphorylation		Uniprot
S138	Phosphorylation		Uniprot
T143	Phosphorylation		Uniprot
S145	Phosphorylation		Uniprot
T149	Phosphorylation		Uniprot
S153	Phosphorylation		Uniprot
K154	Ubiquitination		Uniprot
Y159	Phosphorylation		Uniprot
R162	Methylation		Uniprot
T166	Phosphorylation		Uniprot
S168	Phosphorylation		Uniprot
S172	Phosphorylation		Uniprot
K174	Ubiquitination		Uniprot
Y183	Phosphorylation		Uniprot
T196	Phosphorylation		Uniprot
T199	Phosphorylation		Uniprot
Y200	Phosphorylation		Uniprot
Y208	Phosphorylation		Uniprot
K216	Ubiquitination		Uniprot
T218	Phosphorylation		Uniprot
T219	Phosphorylation		Uniprot
T221	Phosphorylation		Uniprot
Y222	Phosphorylation		Uniprot
S234	Phosphorylation		Uniprot
C239	S-Nitrosylation		Uniprot
K252	Sumoylation		Uniprot
K252	Ubiquitination		Uniprot
T274	Phosphorylation		Uniprot
S275	Phosphorylation	Q5TCY1 (TTBK1)	Uniprot
R276	Methylation		Uniprot
S278	Phosphorylation		Uniprot
Y281	Phosphorylation		Uniprot
T285	Phosphorylation		Uniprot
T290	Phosphorylation		Uniprot
K297	Ubiquitination		Uniprot
C303	S-Nitrosylation		Uniprot
Y310	Phosphorylation		Uniprot
T312	Phosphorylation		Uniprot
R318	Methylation		Uniprot
S322	Phosphorylation		Uniprot
K324	Acetylation		Uniprot
K324	Sumoylation		Uniprot
K324	Ubiquitination		Uniprot
K336	Acetylation		Uniprot
K336	Ubiquitination		Uniprot
S338	Phosphorylation		Uniprot
S339	Phosphorylation		Uniprot
Y340	Phosphorylation		Uniprot
K350	Sumoylation		Uniprot
K350	Ubiquitination		Uniprot
T351	Phosphorylation		Uniprot
K362	Ubiquitination		Uniprot
T366	Phosphorylation		Uniprot
S371	Phosphorylation		Uniprot
K379	Acetylation		Uniprot
K379	Ubiquitination		Uniprot
S382	Phosphorylation		Uniprot
T386	Phosphorylation		Uniprot
K392	Ubiquitination		Uniprot
T399	Phosphorylation		Uniprot
Y422	Phosphorylation		Uniprot
Y425	Phosphorylation		Uniprot
T429	Phosphorylation		Uniprot

Research Backgrounds

P07437_TBB5_HUMAN

Function:

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

PTMs:

Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold.

Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).

Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.

Subcellular Location:

Cytoplasm>Cytoskeleton.

Tissue Specificity:

Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

Subunit Structure:

Heterodimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells. Interacts with PIFO. Interacts with DIAPH1. Interacts with MX1 (By similarity). May interact with RNABP10 (By similarity). Interacts with CFAP157 (By similarity).

Family&Domains:

The highly acidic C-terminal region may bind cations such as calcium.

Belongs to the tubulin family.

Research Fields

· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Gap junction. (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

References

1). Network Pharmacology and In Vitro Experimental Verification Reveal the Mechanism of the Hirudin in Suppressing Myocardial Hypertrophy. Frontiers in Pharmacology, 2022 (PubMed: 35784743) [IF=5.6]

Application: WB Species: Rat Sample: H9c2 cells

FIGURE 8 H9c2 cells were treated with 1 μM AngII, (0.6, 1.2 mM) hirudin and 10 μM losartan for 24 h, respectively. (A) The expression of STAT3, MAPK1 and IL-6 were analyzed by qRT-PCR, n = 6. (B) The IL-6 in cell supernatant was assayed by ELISA, n = 5. (C) Western blot for the expression of proteins including STAT3, p-STAT3, MAPK1, p-MAPK1 and IL-6. (D) Quantified by western blot analysis and normalized to control, n = 3. Bars represent the mean ± SD, *p < 0.05, **p < 0.01.

2). Expression of Retinal G Protein-Coupled Receptor, a Member of the Opsin Family, in Human Skin Cells and Its Mediation of the Cellular Functions of Keratinocytes. Frontiers in Cell and Developmental Biology, 2022 (PubMed: 35445026) [IF=5.5]

3). Buyang Huanwu Decoction improves synaptic plasticity of ischemic stroke by regulating the cAMP/PKA/CREB pathway. Journal of ethnopharmacology, 2024 (PubMed: 39089658) [IF=5.4]

4). Identification and Validation of Genes Related to Macrophage Polarization and Cell Death Modes Under Mycobacterium tuberculosis Infection. Journal of Inflammation Research, 2024 [IF=4.5]

Application: WB Species: Human Sample:

Figure 7 Macrophage polarization. (A) The flowchart of inducing THP-1 polarization into M1/M2 Mφ. Created with BioRender.com. (B) PCR validation of M1/M2 Mφ marker genes. Compare data between multiple groups using one way ANOVA. (C) WB validation of M1/M2 Mφ marker genes. Compare data between multiple groups using one way ANOVA. The statistical chart shows the analysis results of three independent repeated experiments.

5). Reduced endometrial expression of histone deacetylase 3 (HDAC3) in women with adenomyosis who complained of heavy menstrual bleeding. Reproductive BioMedicine Online, 2023 (PubMed: 37690341) [IF=4.0]

6). Reduced endometrial expression of histone deacetylase 3 in women with adenomyosis who complained of heavy menstrual bleeding. Reproductive biomedicine online, 2023 (PubMed: 37690341) [IF=4.0]

7). Roles of Rufy3 in experimental subarachnoid hemorrhage-induced early brain injury via accelerating neuronal axon repair and synaptic plasticity. Molecular Brain, 2022 (PubMed: 35461284) [IF=3.6]

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beta Tubulin Antibody - #DF7967