RRAGA Antibody - #DF7957

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Product: RRAGA Antibody
Catalog: DF7957
Description: Rabbit polyclonal antibody to RRAGA
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Sheep, Rabbit, Chicken, Xenopus
Mol.Wt.: 37 kDa; 37kD(Calculated).
Uniprot: Q7L523
RRID: AB_2841355

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
RRAGA Antibody detects endogenous levels of total RRAGA.
RRID:
AB_2841355
Cite Format: Affinity Biosciences Cat# DF7957, RRID:AB_2841355.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Adenovirus E3 14.7 kDa-interacting protein 1; FIP-1; Rag A; RagA; Ras-related GTP-binding protein A; RRAGA; RRAGA_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q7L523(RRAGA_HUMAN) >>Visit HPA database.

Gene(ID):
RRAGA(10670)
Expression:
Q7L523 RRAGA_HUMAN:

Ubiquitously expressed with highest levels of expression in skeletal muscle, heart, and brain.

Sequence:
MPNTAMKKKVLLMGKSGSGKTSMRSIIFANYIARDTRRLGATIDVEHSHVRFLGNLVLNLWDCGGQDTFMENYFTSQRDNIFRNVEVLIYVFDVESRELEKDMHYYQSCLEAILQNSPDAKIFCLVHKMDLVQEDQRDLIFKEREEDLRRLSRPLECACFRTSIWDETLYKAWSSIVYQLIPNVQQLEMNLRNFAQIIEADEVLLFERATFLVISHYQCKEQRDVHRFEKISNIIKQFKLSCSKLAASFQSMEVRNSNFAAFIDIFTSNTYVMVVMSDPSIPSAATLINIRNARKHFEKLERVDGPKHSLLMR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Horse
0
Dog
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q7L523 As Substrate

Site PTM Type Enzyme
K15 Ubiquitination
S18 Phosphorylation
T21 Phosphorylation
S22 Phosphorylation
S25 Phosphorylation
Y31 Phosphorylation
T36 Phosphorylation
T42 Phosphorylation
S96 Phosphorylation
K128 Ubiquitination
K142 Ubiquitination
K220 Ubiquitination
K230 Ubiquitination
K236 Ubiquitination
K239 Ubiquitination
K244 Ubiquitination
K299 Ubiquitination
K307 Ubiquitination
S309 Phosphorylation

Research Backgrounds

Function:

Guanine nucleotide-binding protein that plays a crucial role in the cellular response to amino acid availability through regulation of the mTORC1 signaling cascade. Forms heterodimeric Rag complexes with RRAGC or RRAGD and cycles between an inactive GDP-bound and an active GTP-bound form. In its active form participates in the relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. Involved in the RCC1/Ran-GTPase pathway. May play a direct role in a TNF-alpha signaling pathway leading to induction of cell death. May alternatively act as a cellular target for adenovirus E3-14.7K, an inhibitor of TNF-alpha functions, thereby affecting cell death.

PTMs:

Ubiquitinated. 'Lys-68'-linked polyubiquitination of the GDP-bound inactive form of RRAGA by RNF152 is increased in response to amino acid starvation. Polyubiquitination promotes interaction with the GATOR1 complex. This does not affect RRAGA degradation.

Subcellular Location:

Cytoplasm. Nucleus. Lysosome.
Note: Predominantly cytoplasmic. May shuttle between the cytoplasm and nucleus, depending on the bound nucleotide state (PubMed:8995684, PubMed:9394008). Colocalizes in vivo with adenovirus E3-14.7K mainly to the cytoplasm especially near the nuclear membrane and in discrete foci on or near the plasma membrane (PubMed:8995684).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed with highest levels of expression in skeletal muscle, heart, and brain.

Subunit Structure:

Can occur as a homodimer or as a heterodimer with RRAGC or RRAGD in a sequence-independent manner; heterodimerization stabilizes proteins of the heterodimer. In complex with RRAGC, but not with RRAGB, interacts with RPTOR. The GTP-bound form of RRAGA interacts with NOL8. Interacts with SH3BP4; the interaction with this negative regulator is most probably direct, preferentially occurs with the inactive GDP-bound form of RRAGA and is negatively regulated by amino acids. The Rag heterodimer interacts with SLC38A9; the probable amino acid sensor. Interacts (inactive GDP-bound form) with RNF152; stimulated by amino acid starvation. Interacts (polyubiquitinated) with the GATOR1 complex; inactivates RRAGA. Interacts (polyubiquitinated) with TSC2. Interacts with SESN1, SESN2 AND SESN3. Interacts with PIP4P1 (By similarity). Interacts with GPR137B.

(Microbial infection) Interacts with adenovirus E3 14.7 kDa protein.

Family&Domains:

Belongs to the GTR/RAG GTP-binding protein family.

Research Fields

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

References

1). A leucine-rich diet modulates the mTOR cell signalling pathway in the gastrocnemius muscle under different Walker-256 tumour growth conditions. BMC CANCER, 2019 (PubMed: 30975087) [IF=3.8]

Application: WB    Species: rat    Sample:

Fig. 3 | mTOR, p70S6K-1, mTOR/p70S6K ratio and RAG-A (arbitrary units). The animals were euthanised at different times during tumour development,including the 7th, 14th, and 21st days of the experiment. Legend: C, control group; W, Walker tumour-bearing group; L, rats fed a leucine-rich diet; WL,tumour-bearing rats fed a leucine-rich diet. Graphics represent mean ± standard deviation. Statistical significance is presented in Table 1 after two-way Anova analysis, followed by the Fisher’s LSD multiple comparison test; P < 0.05

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