PCYT1A Antibody - #DF7927

(2)  
Product: PCYT1A Antibody
Catalog: DF7927
Description: Rabbit polyclonal antibody to PCYT1A
Application: WB IHC
Reactivity: Human
Prediction: Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 42 kDa; 42kD(Calculated).
Uniprot: P49585
RRID: AB_2841339

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
PCYT1A Antibody detects endogenous levels of total PCYT1A.
RRID:
AB_2841339
Cite Format: Affinity Biosciences Cat# DF7927, RRID:AB_2841339.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CCT A; CCT alpha; CCT-alpha; Choline phosphate cytidylyltransferase A; Choline-phosphate cytidylyltransferase A; CT A; CT; CTP:phosphocholine cytidylyltransferase A; CTPCT; PCY1A_HUMAN; PCYT1; Pcyt1a; Phosphate cytidylyltransferase 1 choline alpha; Phosphate cytidylyltransferase 1 choline alpha isoform; Phosphorylcholine transferase A;

Immunogens

Immunogen:
Uniprot:

P49585(PCY1A_HUMAN) >>Visit HPA database.

Gene(ID):
PCYT1A(5130)
Sequence:
MDAQCSAKVNARKRRKEAPGPNGATEEDGVPSKVQRCAVGLRQPAPFSDEIEVDFSKPYVRVTMEEASRGTPCERPVRVYADGIFDLFHSGHARALMQAKNLFPNTYLIVGVCSDELTHNFKGFTVMNENERYDAVQHCRYVDEVVRNAPWTLTPEFLAEHRIDFVAHDDIPYSSAGSDDVYKHIKEAGMFAPTQRTEGISTSDIITRIVRDYDVYARRNLQRGYTAKELNVSFINEKKYHLQERVDKVKKKVKDVEEKSKEFVQKVEEKSIDLIQKWEEKSREFIGSFLEMFGPEGALKHMLKEGKGRMLQAISPKQSPSSSPTRERSPSPSFRWPFSGKTSPPCSPANLSRHKAAAYDISEDEED

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Pig
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P49585 As Substrate

Site PTM Type Enzyme
M1 Acetylation
K8 Acetylation
K8 Sumoylation
K8 Ubiquitination
K33 Ubiquitination
K57 Acetylation
K57 Ubiquitination
Y141 Phosphorylation
Y173 Phosphorylation
Y182 Phosphorylation
K183 Ubiquitination
K186 Ubiquitination
T197 Phosphorylation
Y216 Phosphorylation
K228 Ubiquitination
S233 Phosphorylation
K238 Ubiquitination
K261 Ubiquitination
K266 Sumoylation
K270 Ubiquitination
S271 Phosphorylation
K277 Ubiquitination
S315 Phosphorylation P27361 (MAPK3) , P28482 (MAPK1)
S319 Phosphorylation
S321 Phosphorylation
S322 Phosphorylation
S323 Phosphorylation
T325 Phosphorylation
S329 Phosphorylation
S331 Phosphorylation
S333 Phosphorylation
S339 Phosphorylation
T342 Phosphorylation
S343 Phosphorylation
S347 Phosphorylation
S352 Phosphorylation
Y359 Phosphorylation
S362 Phosphorylation

Research Backgrounds

Function:

Controls phosphatidylcholine synthesis.

PTMs:

The serine residues of the C-terminus are phosphorylated. The inactive soluble form is stabilized by phosphorylation, the active membrane bound form is promoted by anionic lipids or diacylglycerol, and is stabilized by dephosphorylation (By similarity).

Monoubiquitinated by the SCF(FBXL2) complex, leading to proteasomal degradation.

Subcellular Location:

Cytoplasm>Cytosol. Membrane>Peripheral membrane protein.
Note: It can interconvert between an inactive cytosolic form and an active membrane-bound form.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the cytidylyltransferase family.

Research Fields

· Human Diseases > Cancers: Overview > Choline metabolism in cancer.   (View pathway)

· Metabolism > Metabolism of other amino acids > Phosphonate and phosphinate metabolism.

· Metabolism > Lipid metabolism > Glycerophospholipid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

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