TMPRSS2 Antibody - #DF7917

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Product: TMPRSS2 Antibody
Catalog: DF7917
Description: Rabbit polyclonal antibody to TMPRSS2
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Dog
Mol.Wt.: 54 kDa; 54kD(Calculated).
Uniprot: O15393
RRID: AB_2841332

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(92%), Bovine(100%), Horse(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
TMPRSS2 Antibody detects endogenous levels of total TMPRSS2.
RRID:
AB_2841332
Cite Format: Affinity Biosciences Cat# DF7917, RRID:AB_2841332.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

D16Ertd61e; Epitheliasin; FLJ41954; MGC6821; PP9284; PRSS10; Serine protease 10; TMPRSS2; TMPRSS2 ERG FUSION GENE, INCLUDED; TMPRSS2 ETV1 FUSION GENE, INCLUDED; TMPS2_HUMAN; Transmembrane protease serine 2 catalytic chain; Transmembrane protease, serine 2; Transmembrane protease, serine 2, EC 3.4.219;

Immunogens

Immunogen:
Uniprot:

O15393(TMPS2_HUMAN) >>Visit HPA database.

Gene(ID):
TMPRSS2(7113)
Expression:
O15393 TMPS2_HUMAN:

Highly expressed in prostate epithelial cells and in prostate cancers. Expressed in type II pneumocytes in the lung (at protein level). Expressed strongly in small intestine. Also expressed in colon, stomach and salivary gland.

Sequence:
MALNSGSPPAIGPYYENHGYQPENPYPAQPTVVPTVYEVHPAQYYPSPVPQYAPRVLTQASNPVVCTQPKSPSGTVCTSKTKKALCITLTLGTFLVGAALAAGLLWKFMGSKCSNSGIECDSSGTCINPSNWCDGVSHCPGGEDENRCVRLYGPNFILQVYSSQRKSWHPVCQDDWNENYGRAACRDMGYKNNFYSSQGIVDDSGSTSFMKLNTSAGNVDIYKKLYHSDACSSKAVVSLRCIACGVNLNSSRQSRIVGGESALPGAWPWQVSLHVQNVHVCGGSIITPEWIVTAAHCVEKPLNNPWHWTAFAGILRQSFMFYGAGYQVEKVISHPNYDSKTKNNDIALMKLQKPLTFNDLVKPVCLPNPGMMLQPEQLCWISGWGATEEKGKTSEVLNAAKVLLIETQRCNSRYVYDNLITPAMICAGFLQGNVDSCQGDSGGPLVTSKNNIWWLIGDTSWGSGCAKAYRPGVYGNVMVFTDWIYRQMRADG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Dog
100
Pig
92
Chicken
58
Sheep
0
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15393 As Substrate

Site PTM Type Enzyme
Y44 Phosphorylation
Y45 Phosphorylation
Y52 Phosphorylation
T58 Phosphorylation
S61 Phosphorylation
T341 Phosphorylation

Research Backgrounds

Function:

Serine protease that proteolytically cleaves and activates the viral spike glycoproteins which facilitate virus-cell membrane fusions; spike proteins are synthesized and maintained in precursor intermediate folding states and proteolysis permits the refolding and energy release required to create stable virus-cell linkages and membrane coalescence. Facilitates human SARS coronavirus (SARS-CoV) infection via two independent mechanisms, proteolytic cleavage of ACE2, which might promote viral uptake, and cleavage of coronavirus spike glycoprotein which activates the glycoprotein for cathepsin L-independent host cell entry. Proteolytically cleaves and activates the spike glycoproteins of human coronavirus 229E (HCoV-229E) and human coronavirus EMC (HCoV-EMC) and the fusion glycoproteins F0 of Sendai virus (SeV), human metapneumovirus (HMPV), human parainfluenza 1, 2, 3, 4a and 4b viruses (HPIV). Essential for spread and pathogenesis of influenza A virus (strains H1N1, H3N2 and H7N9); involved in proteolytic cleavage and activation of hemagglutinin (HA) protein which is essential for viral infectivity.

PTMs:

Proteolytically processed; by an autocatalytic mechanism.

Subcellular Location:

Cell membrane>Single-pass type II membrane protein.

Secreted.
Note: Activated by cleavage and secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in prostate epithelial cells and in prostate cancers. Expressed in type II pneumocytes in the lung (at protein level). Expressed strongly in small intestine. Also expressed in colon, stomach and salivary gland.

Subunit Structure:

The catalytically active form interacts with ACE2.

Family&Domains:

Belongs to the peptidase S1 family.

Research Fields

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

References

1). Revealing the mechanism of ethyl acetate extracts of Semen Impatientis against prostate cancer based on network pharmacology and transcriptomics. Journal of ethnopharmacology, 2024 (PubMed: 38643863) [IF=5.4]

Application: WB    Species: Human    Sample:

Fig. 6. Network pharmacology analysis and experiments were utilized to explore the molecular mechanism of EAESI in PCa. (A) Disease-related targets for PCa were selected from DrugBank, GeneCards, OMIM, and the TTD. (B) Venn diagram showing the intersection of the drug targets and the disease-related targets. (C) A compound–target network was constructed. (D) The common targets of Semen Impatientis and PCa were used to construct the PPI network. (E) Topological analysis of the PPI network was employed to identify the hub genes. (F–G) The impacts of EAESI on the expression and transcriptional activity of AR in LNCaP and 22RV1 cells were evaluated by RT‒qPCR (F) and Western blot (G) assays. The data are presented as the means ± SDs. *P < 0.05, **P < 0.01, ***P < 0.001 compared with the 0 mg/mL group.

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