PAX7 Antibody - #DF7915

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Product: PAX7 Antibody
Catalog: DF7915
Description: Rabbit polyclonal antibody to PAX7
Application: WB
Reactivity: Human
Prediction: Pig, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 57 kDa; 55kD(Calculated).
Uniprot: P23759
RRID: AB_2841330

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(88%)
Clonality:
Polyclonal
Specificity:
PAX7 Antibody detects endogenous levels of total PAX7.
RRID:
AB_2841330
Cite Format: Affinity Biosciences Cat# DF7915, RRID:AB_2841330.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FLJ37460; HUP1; OTTHUMP00000002534; Paired box 7; Paired box gene 7; Paired box homeotic gene 7; Paired box protein Pax-7; Paired domain gene 7; Paired domain gene HuP1; Pax7; PAX7 transcriptional factor; PAX7/FKHR fusion gene, included; PAX7_HUMAN; PAX7B; RGD1564360; RMS2;

Immunogens

Immunogen:
Uniprot:

P23759(PAX7_HUMAN) >>Visit HPA database.

Gene(ID):
PAX7(5081)
Sequence:
MAALPGTVPRMMRPAPGQNYPRTGFPLEVSTPLGQGRVNQLGGVFINGRPLPNHIRHKIVEMAHHGIRPCVISRQLRVSHGCVSKILCRYQETGSIRPGAIGGSKPRQVATPDVEKKIEEYKRENPGMFSWEIRDRLLKDGHCDRSTVPSGLVSSISRVLRIKFGKKEEEDEADKKEDDGEKKAKHSIDGILGDKGNRLDEGSDVESEPDLPLKRKQRRSRTTFTAEQLEELEKAFERTHYPDIYTREELAQRTKLTEARVQVWFSNRRARWRKQAGANQLAAFNHLLPGGFPPTGMPTLPPYQLPDSTYPTTTISQDGGSTVHRPQPLPPSTMHQGGLAAAAAAADTSSAYGARHSFSSYSDSFMNPAAPSNHMNPVSNGLSPQVMSILGNPSAVPPQPQADFSISPLHGGLDSATSISASCSQRADSIKPGDSLPTSQAYCPPTYSTTGYSVDPVAGYQYGQYGQTAVDYLAKNVSLSTQRRMKLGEHSAVLGLLPVETGQAY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
88
Horse
0
Bovine
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P23759 As Substrate

Site PTM Type Enzyme
Y121 Phosphorylation
T147 Phosphorylation
K176 Acetylation
S203 Phosphorylation
S207 Phosphorylation

Research Backgrounds

Function:

Transcription factor playing a role in myogenesis through regulation of muscle precursor cells proliferation.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Can bind to DNA as a heterodimer with PAX3. Interacts with PAXBP1; the interaction links PAX7 to a WDR5-containing histone methyltransferase complex (By similarity). Interacts with DAXX.

Family&Domains:

Belongs to the paired homeobox family.

Research Fields

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

References

1). Sympathetic activity is correlated with satellite cell aging and myogenesis via β2-adrenoceptor. Stem Cell Research & Therapy, 2021 (PubMed: 34530910) [IF=7.5]

Application: IF/ICC    Species: Mice    Sample: SC cells

Fig. 2 The distributions of β-ADR and β2-ADR were decreased in aged SCs. A, B WB analysis showed low expression of β1-ADR, high expression of β2-ADR, and almost no expression of β3-ADR (n = 5) (F = 18.95, P < 0.001). C, D The β2-ADR expression in the SCs of aged mice was significantly lower than that in the SCs of young mice (n = 5) (t = 6.321, P < 0.001). E, F Immunofluorescence staining showed that the expression of β2-ADR in the TA muscles of aged mice was lower than that in the TA muscles of young mice (n = 8) (t = 1.31, P = 0.014). G–J Immunofluorescence staining showed that the expression of PAX7 was not significantly decreased in the SCs of aged mice in vitro (n = 8) (t = 0.18, P = 0.25) but that the number of PAX7− SCs (white arrow) in the proliferative phase was decreased significantly (n = 8) (t = 2.08, P = 0.007). The expression of β2-ADR was lower in aged mice than that in young mice (n = 8) (t = 1.67, P = 0.011). K The tissue concentration of NE was higher in young mice than in aged mice in both the noninjury and injury states (t = 3.07 and 2.97, P = 0.009 and 0.012). Independent samples t tests and one-way ANOVA with Tukey’s test, ns, not significant, *P < 0.05; **P < 0.001

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