WARS Antibody - #DF7913

(6)  
Product: WARS Antibody
Catalog: DF7913
Description: Rabbit polyclonal antibody to WARS
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 25 kDa; 53kD(Calculated).
Uniprot: P23381
RRID: AB_2841329

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Zebrafish(92%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
WARS Antibody detects endogenous levels of total WARS.
RRID:
AB_2841329
Cite Format: Affinity Biosciences Cat# DF7913, RRID:AB_2841329.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Gamma 2; GAMMA-2; Gamma2; hWRS; IFI 53; IFI53; IFP 53; IFP53; Interferon induced protein 53; Interferon-induced protein 53; SYWC_HUMAN; T2-TrpRS; TrpRS; Tryptophan tRNA ligase, cytoplasmic; Tryptophan tRNA ligase 1 cytoplasmic; Tryptophan tRNA ligase; Tryptophan--tRNA ligase; Tryptophanyl tRNA synthetase; Tryptophanyl tRNA synthetase cytoplasmic; WARS; WARS protein; WRS;

Immunogens

Immunogen:
Uniprot:

P23381(SYWC_HUMAN) >>Visit HPA database.

Gene(ID):
WARS(7453)
Sequence:
MPNSEPASLLELFNSIATQGELVRSLKAGNASKDEIDSAVKMLVSLKMSYKAAAGEDYKADCPPGNPAPTSNHGPDATEAEEDFVDPWTVQTSSAKGIDYDKLIVRFGSSKIDKELINRIERATGQRPHHFLRRGIFFSHRDMNQVLDAYENKKPFYLYTGRGPSSEAMHVGHLIPFIFTKWLQDVFNVPLVIQMTDDEKYLWKDLTLDQAYSYAVENAKDIIACGFDINKTFIFSDLDYMGMSSGFYKNVVKIQKHVTFNQVKGIFGFTDSDCIGKISFPAIQAAPSFSNSFPQIFRDRTDIQCLIPCAIDQDPYFRMTRDVAPRIGYPKPALLHSTFFPALQGAQTKMSASDPNSSIFLTDTAKQIKTKVNKHAFSGGRDTIEEHRQFGGNCDVDVSFMYLTFFLEDDDKLEQIRKDYTSGAMLTGELKKALIEVLQPLIAEHQARRKEVTDEIVKEFMTPRKLSFDFQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Zebrafish
92
Pig
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P23381 As Substrate

Site PTM Type Enzyme
S4 Phosphorylation
K27 Ubiquitination
S32 Phosphorylation
K33 Acetylation
K33 Ubiquitination
S38 Phosphorylation
K41 Ubiquitination
S45 Phosphorylation
K47 Ubiquitination
K51 Ubiquitination
K59 Ubiquitination
K96 Ubiquitination
K102 Ubiquitination
K111 Ubiquitination
K114 Ubiquitination
S139 Phosphorylation
K154 Ubiquitination
K200 Acetylation
K204 Acetylation
K204 Ubiquitination
K220 Ubiquitination
K253 Ubiquitination
K256 Ubiquitination
T259 Phosphorylation
K264 Ubiquitination
S272 Phosphorylation
T301 Phosphorylation
Y316 Phosphorylation
K349 Ubiquitination
S351 Phosphorylation
S353 Phosphorylation
S358 Phosphorylation
K366 Acetylation
K366 Ubiquitination
S378 Phosphorylation
R381 Methylation
T383 Phosphorylation
K431 Acetylation
K431 Ubiquitination
K432 Ubiquitination
K450 Ubiquitination
T453 Phosphorylation
K458 Ubiquitination
K465 Ubiquitination
S467 Phosphorylation

Research Backgrounds

Function:

Isoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression.

PTMs:

Proteolytic cleavage generates 2 forms; T1-TrpRS and T2-TrpRS.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer. Isoform 1 and isoform 2 interact with an oxidized form of GAPDH. GAPDH stimulates the aminoacylation activity of isoform 2.

Family&Domains:

Belongs to the class-I aminoacyl-tRNA synthetase family.

Research Fields

· Genetic Information Processing > Translation > Aminoacyl-tRNA biosynthesis.

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