ROR2 Antibody | Affinity Biosciences

WB
Cites

1/2

Western blot analysis of ROR2 using HeLa whole lysates.

Fig 8 Immunohistochemical staining of groups (40×).

Product:	ROR2 Antibody
Catalog:	DF7906
Description:	Rabbit polyclonal antibody to ROR2
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Zebrafish, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	105kDa; 105kD(Calculated).
Uniprot:	Q01974
RRID:	AB_2841323

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:200-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(88%), Zebrafish(88%), Horse(88%), Sheep(88%), Rabbit(100%), Dog(88%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

ROR2 Antibody detects endogenous levels of total ROR2.

RRID:

AB_2841323
Cite Format: Affinity Biosciences Cat# DF7906, RRID:AB_2841323.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

BDB; BDB1; Brachydactyly type B; EC 2.7.10.1; MGC163394; Neurotrophic tyrosine kinase; Neurotrophic tyrosine kinase, receptor related 2; NTRKR2; Receptor tyrosine kinase-like orphan receptor 2; receptor-related 2; ROR2; ROR2_HUMAN; Tyrosine protein kinase transmembrane receptor ROR2; Tyrosine-protein kinase transmembrane receptor Ror2;

Immunogens

Immunogen:

Uniprot:

Q01974(ROR2_HUMAN) >>Visit HPA database.

Gene(ID):

ROR2(4920)

Sequence:

Q01974 ROR2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MARGSALPRRPLLCIPAVWAAAALLLSVSRTSGEVEVLDPNDPLGPLDGQDGPIPTLKGYFLNFLEPVNNITIVQGQTAILHCKVAGNPPPNVRWLKNDAPVVQEPRRIIIRKTEYGSRLRIQDLDTTDTGYYQCVATNGMKTITATGVLFVRLGPTHSPNHNFQDDYHEDGFCQPYRGIACARFIGNRTIYVDSLQMQGEIENRITAAFTMIGTSTHLSDQCSQFAIPSFCHFVFPLCDARSRTPKPRELCRDECEVLESDLCRQEYTIARSNPLILMRLQLPKCEALPMPESPDAANCMRIGIPAERLGRYHQCYNGSGMDYRGTASTTKSGHQCQPWALQHPHSHHLSSTDFPELGGGHAYCRNPGGQMEGPWCFTQNKNVRMELCDVPSCSPRDSSKMGILYILVPSIAIPLVIACLFFLVCMCRNKQKASASTPQRRQLMASPSQDMEMPLINQHKQAKLKEISLSAVRFMEELGEDRFGKVYKGHLFGPAPGEQTQAVAIKTLKDKAEGPLREEFRHEAMLRARLQHPNVVCLLGVVTKDQPLSMIFSYCSHGDLHEFLVMRSPHSDVGSTDDDRTVKSALEPPDFVHLVAQIAAGMEYLSSHHVVHKDLATRNVLVYDKLNVKISDLGLFREVYAADYYKLLGNSLLPIRWMAPEAIMYGKFSIDSDIWSYGVVLWEVFSYGLQPYCGYSNQDVVEMIRNRQVLPCPDDCPAWVYALMIECWNEFPSRRPRFKDIHSRLRAWGNLSNYNSSAQTSGASNTTQTSSLSTSPVSNVSNARYVGPKQKAPPFPQPQFIPMKGQIRPMVPPPQLYVPVNGYQPVPAYGAYLPNFYPVQIPMQMAPQQVPPQMVPKPSSHHSGSGSTSTGYVTTAPSNTSMADRAALLSEGADDTQNAPEDGAQSTVQEAEEEEEGSVPETELLGDCDTLQVDEAQVQLEA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Xenopus

100

Chicken

100

Rabbit

100

Pig

Horse

Sheep

Dog

Zebrafish

Bovine

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q01974 As Substrate

Q01974

Site	PTM Type	Enzyme	Source
K97	Ubiquitination		Uniprot
T157	O-Glycosylation		Uniprot
T438	Phosphorylation		Uniprot
S447	Phosphorylation		Uniprot
S449	Phosphorylation		Uniprot
K466	Ubiquitination		Uniprot
S469	Phosphorylation		Uniprot
K489	Ubiquitination		Uniprot
K507	Ubiquitination		Uniprot
K512	Ubiquitination		Uniprot
Y555	Phosphorylation		Uniprot
Y624	Phosphorylation		Uniprot
K626	Ubiquitination		Uniprot
Y645	Phosphorylation		Uniprot
Y646	Phosphorylation		Uniprot
K647	Ubiquitination		Uniprot
Y666	Phosphorylation		Uniprot
Y755	Phosphorylation		Uniprot
S758	Phosphorylation		Uniprot
S762	Phosphorylation		Uniprot
S782	Phosphorylation		Uniprot
K792	Ubiquitination		Uniprot
Y824	Phosphorylation		Uniprot
S864	Phosphorylation	P49841 (GSK3B)	Uniprot
Y873	Phosphorylation		Uniprot

Research Backgrounds

Q01974_ROR2_HUMAN

Function:

Tyrosine-protein kinase receptor which may be involved in the early formation of the chondrocytes. It seems to be required for cartilage and growth plate development (By similarity). Phosphorylates YWHAB, leading to induction of osteogenesis and bone formation. In contrast, has also been shown to have very little tyrosine kinase activity in vitro. May act as a receptor for wnt ligand WNT5A which may result in the inhibition of WNT3A-mediated signaling.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Subunit Structure:

Homodimer; promotes osteogenesis. Binds YWHAB. Interacts with WTIP (By similarity).

Family&Domains:

Belongs to the protein kinase superfamily. Tyr protein kinase family. ROR subfamily.

References

1). The Protective Effects of Parathyroid Hormone (1-34) on Cartilage and Subchondral Bone Through Down-Regulating JAK2/STAT3 and WNT5A/ROR2 in a Collagenase-Induced Osteoarthritis Mouse Model. Orthopaedic Surgery, 2021 (PubMed: 34105258) [IF=2.1]

Application: IHC Species: Mouse Sample: cartilage tissue

Fig 8 Immunohistochemical staining of groups (40×). This figure demonstrates the expression of JAK2, STAT3, WNT5A, and ROR2 in load-bearing areas of subchondral bone among groups. The positive expression of JAK2, STAT3, WNT5A, and ROR2 was defined as brown-yellow stain. Data are presented as the mean SD. Scale bars = 50 μm. Group A, sham group; Group B, the collagenase + saline injection group; Group C, the collagenase + PTH (1-34) treatment group. PTH, parathyroid hormone.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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ROR2 Antibody - #DF7906