APOA2 Antibody - #DF7905

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Product: APOA2 Antibody
Catalog: DF7905
Description: Rabbit polyclonal antibody to APOA2
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Sheep, Dog
Mol.Wt.: 11 kDa; 11kD(Calculated).
Uniprot: P02652
RRID: AB_2841322

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(80%), Sheep(80%), Dog(83%)
Clonality:
Polyclonal
Specificity:
APOA2 Antibody detects endogenous levels of total APOA2.
RRID:
AB_2841322
Cite Format: Affinity Biosciences Cat# DF7905, RRID:AB_2841322.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

APO A2; Apo AII; Apo-AII; APOA 2; ApoA II; ApoA-II; APOA2; APOA2_HUMAN; APOAII; Apolipoprotein A II; Apolipoprotein A-II(1-76); Apolipoprotein A2; Apolipoprotein AII; ApolipoproteinA II; OTTHUMP00000032244;

Immunogens

Immunogen:
Uniprot:

P02652(APOA2_HUMAN) >>Visit HPA database.

Gene(ID):
APOA2(336)
Expression:
P02652 APOA2_HUMAN:

Plasma; synthesized in the liver and intestine.

Sequence:
MKLLAATVLLLTICSLEGALVRRQAKEPCVESLVSQYFQTVTDYGKDLMEKVKSPELQAEAKSYFEKSKEQLTPLIKKAGTELVNFLSYFVELGTQPATQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
83
Bovine
80
Sheep
80
Rabbit
75
Horse
67
Pig
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P02652 As Substrate

Site PTM Type Enzyme
S32 Phosphorylation
S35 Phosphorylation
Y37 Phosphorylation
T42 O-Glycosylation
S54 Phosphorylation
K67 Acetylation
S68 Phosphorylation

Research Backgrounds

Function:

May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism.

PTMs:

Phosphorylation sites are present in the extracellular medium.

Apolipoprotein A-II is O-glycosylated.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Plasma; synthesized in the liver and intestine.

Subunit Structure:

Monomer. Homodimer; disulfide-linked. Also forms a disulfide-linked heterodimer with APOD.Interacts with APOA1BP and NDRG1.

(Microbial infection) Interacts with HCV core protein.

Family&Domains:

Belongs to the apolipoprotein A2 family.

Research Fields

· Organismal Systems > Endocrine system > PPAR signaling pathway.

· Organismal Systems > Digestive system > Cholesterol metabolism.

References

1). Neural stem cell-derived exosomes regulate neural stem cell differentiation through miR-9-Hes1 axis. Frontiers in Cell and Developmental Biology (PubMed: 34055767) [IF=5.5]

Application: WB    Species: Mouse    Sample: neural stem cells (NSC)

FIGURE 1 Characterization of exosomes (EXOs). (A) Purified exosomes were observed under transmission electron microscopy (TEM) using negative staining. (B) The levels of positive exosomal markers TSG101, CD9, and Flotillin-1 in protein lysates of neural stem cells (NSC)-derived exosome pellets were determined by western blotting. (C) The levels of negative exosomal markers APOA1 and APOA2 in protein lysates of NSC-derived exosome pellets and NSCs were determined by western blotting. (D,E) Particle-size distribution of exosomes was determined by NanoSight analysis (NTA) in panel (D) and NanoFCM in panel (E) technologies. Scale bar 200 nm in panel (A).

Application: WB    Species: mouse    Sample: NSCs

FIGURE 1 | Characterization of exosomes (EXOs). (C) The levels of negative exosomal markers APOA1 and APOA2 in protein lysates of NSC-derived exosome pellets and NSCs were determined by western blotting.
2). Neural stem cell-derived exosomal FTO protects neuron from microglial inflammatory injury by inhibiting microglia NRF2 mRNA m6A modification. Journal of neurogenetics (PubMed: 37812019) [IF=1.9]

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