Product: CITED1 Antibody
Catalog: DF7903
Description: Rabbit polyclonal antibody to CITED1
Application: WB
Reactivity: Human
Mol.Wt.: 20 kDa; 20kD(Calculated).
Uniprot: Q99966
RRID: AB_2841320

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
CITED1 Antibody detects endogenous levels of total CITED1.
RRID:
AB_2841320
Cite Format: Affinity Biosciences Cat# DF7903, RRID:AB_2841320.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ABCC1; AI316840; AU019144; Cbp/p300-interacting transactivator 1; Cbp/p300-interacting transactivator with GLU/ASP-rich C-terminal domain, 1; Cbp/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain 1; CITE1_HUMAN; CITED1; melanocyte specific gene 1; Melanocyte specific protein 1; Melanocyte-specific protein 1; MSG1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q99966 CITE1_HUMAN:

Expressed only in melanocytes and testis.

Sequence:
MPTTSRPALDVKGGTSPAKEDANQEMSSVAYSNLAVKDRKAVAILHYPGVASNGTKASGAPTSSSGSPIGSPTTTPPTKPPSFNLHPAPHLLASMHLQKLNSQYQGMAAATPGQPGEAGPLQNWDFGAQAGGAESLSPSAGAQSPAIIDSDPVDEEVLMSLVVELGLDRANELPELWLGQNEFDFTADFPSSC

PTMs - Q99966 As Substrate

Site PTM Type Enzyme
K37 Acetylation
S52 Phosphorylation
T55 Phosphorylation
S64 Phosphorylation

Research Backgrounds

Function:

Transcriptional coactivator of the p300/CBP-mediated transcription complex. Enhances SMAD-mediated transcription by strengthening the functional link between the DNA-binding SMAD transcription factors and the p300/CBP transcription coactivator complex. Stimulates estrogen-dependent transactivation activity mediated by estrogen receptors signaling; stabilizes the interaction of estrogen receptor ESR1 and histone acetyltransferase EP300. Positively regulates TGF-beta signaling through its association with the SMAD/p300/CBP-mediated transcriptional coactivator complex. Induces transcription from estrogen-responsive promoters and protection against cell death. Potentiates EGR2-mediated transcriptional activation activity from the ERBB2 promoter. Acts as an inhibitor of osteoblastic mineralization through a cAMP-dependent parathyroid hormone receptor signaling. May play a role in pigmentation of melanocytes. Associates with chromatin to the estrogen-responsive TGF-alpha promoter region in a estrogen-dependent manner.

PTMs:

Phosphorylated. Phosphorylation changes in a cell cycle-dependent manner and reduces its transcriptional coactivator activity.

Subcellular Location:

Nucleus. Cytoplasm.
Note: Shuttles between the nucleus and the cytoplasm by a nuclear export signal and (NES) in a CRM1-dependent manner.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed only in melanocytes and testis.

Subunit Structure:

Interacts (via C-terminus) with CREBBP. Interacts with EGR2 (By similarity). Homodimer. Binds to RBM14. Interacts (via N-terminus) with HSPA8; the interaction suppresses the association of CITED1 with p300/CBP and SMAD-mediated transcription transactivation. Interacts (via C-terminus) with TOX3 (via HGM box); the interaction increases estrogen-response element (ERE)-dependent transcription and protection against cell death. Interacts with ESR1; the interaction occurs in a estrogen-dependent manner (By similarity). Interacts (unphosphorylated form preferentially and via C-terminus) with EP300.

Family&Domains:

Belongs to the CITED family.

References

1). Study on the changes of LHR, FSHR and AR with the development of testis cells in Hu sheep. Animal Reproduction Science, 2023 (PubMed: 37541020) [IF=2.2]

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