ENTPD5 Antibody - #DF7901

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Product: ENTPD5 Antibody
Catalog: DF7901
Description: Rabbit polyclonal antibody to ENTPD5
Application: WB IHC IF/ICC
Reactivity: Human, Monkey
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 47 kDa; 48kD(Calculated).
Uniprot: O75356
RRID: AB_2841318

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Monkey
Prediction:
Pig(100%), Bovine(100%), Horse(92%), Sheep(100%), Rabbit(83%), Dog(92%)
Clonality:
Polyclonal
Specificity:
ENTPD5 Antibody detects endogenous levels of total ENTPD5.
RRID:
AB_2841318
Cite Format: Affinity Biosciences Cat# DF7901, RRID:AB_2841318.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CD39 like 4; AI196558; AI987697; CD39 antigen like 4; CD39 antigen-like 4; CD39 like 4; CD39L4; Ectonucleoside triphosphate diphosphohydrolase 5; ENTP5_HUMAN; Entpd5; ER UDPase; ER-UDPase; GDPase ENTPD5; Guanosine-diphosphatase ENTPD5; MGC163357; MGC163359; mNTPase; NTPDase 5; Nucleoside diphosphatase; PCPH; Pcph proto oncogene protein; Proto oncogene CPH; UDPase ENTPD5; Uridine-diphosphatase ENTPD5;

Immunogens

Immunogen:
Uniprot:

O75356(ENTP5_HUMAN) >>Visit HPA database.

Gene(ID):
ENTPD5(957)
Expression:
O75356 ENTP5_HUMAN:

Expressed in adult liver, kidney, prostate, testis and colon. Much weaker expression in other tissues.

Sequence:
MATSWGTVFFMLVVSCVCSAVSHRNQQTWFEGIFLSSMCPINVSASTLYGIMFDAGSTGTRIHVYTFVQKMPGQLPILEGEVFDSVKPGLSAFVDQPKQGAETVQGLLEVAKDSIPRSHWKKTPVVLKATAGLRLLPEHKAKALLFEVKEIFRKSPFLVPKGSVSIMDGSDEGILAWVTVNFLTGQLHGHRQETVGTLDLGGASTQITFLPQFEKTLEQTPRGYLTSFEMFNSTYKLYTHSYLGFGLKAARLATLGALETEGTDGHTFRSACLPRWLEAEWIFGGVKYQYGGNQEGEVGFEPCYAEVLRVVRGKLHQPEEVQRGSFYAFSYYYDRAVDTDMIDYEKGGILKVEDFERKAREVCDNLENFTSGSPFLCMDLSYITALLKDGFGFADSTVLQLTKKVNNIETGWALGATFHLLQSLGISH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Horse
92
Dog
92
Rabbit
83
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75356 As Substrate

Site PTM Type Enzyme
K98 Acetylation
S227 Phosphorylation
N232 N-Glycosylation
T234 Phosphorylation

Research Backgrounds

Function:

Uridine diphosphatase (UDPase) that promotes protein N-glycosylation and ATP level regulation. UDP hydrolysis promotes protein N-glycosylation and folding in the endoplasmic reticulum, as well as elevated ATP consumption in the cytosol via an ATP hydrolysis cycle. Together with CMPK1 and AK1, constitutes an ATP hydrolysis cycle that converts ATP to AMP and results in a compensatory increase in aerobic glycolysis. The nucleotide hydrolyzing preference is GDP > IDP > UDP, but not any other nucleoside di-, mono- or triphosphates, nor thiamine pyrophosphate. Plays a key role in the AKT1-PTEN signaling pathway by promoting glycolysis in proliferating cells in response to phosphoinositide 3-kinase (PI3K) signaling.

PTMs:

N-glycosylated; high-mannose type (By similarity). Glycosylation is not essential for enzymatic activity.

Subcellular Location:

Endoplasmic reticulum. Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in adult liver, kidney, prostate, testis and colon. Much weaker expression in other tissues.

Subunit Structure:

Exists both as a monomer and a disulfide-linked homodimer, the dimers are enzymatically inactive.

Family&Domains:

Belongs to the GDA1/CD39 NTPase family.

Research Fields

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

Restrictive clause

 

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