Product: FGA Antibody
Catalog: DF7895
Description: Rabbit polyclonal antibody to FGA
Application: WB IHC
Reactivity: Human, Rat
Prediction: Chicken, Xenopus
Mol.Wt.: 95 kDa; 95kD(Calculated).
Uniprot: P02671
RRID: AB_2841316

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Chicken(82%), Xenopus(91%)
Clonality:
Polyclonal
Specificity:
FGA Antibody detects endogenous levels of total FGA.
RRID:
AB_2841316
Cite Format: Affinity Biosciences Cat# DF7895, RRID:AB_2841316.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Ac1873; Fba5e; FGA; Fib; Fib2; FIBA_HUMAN; Fibrinogen alpha chain; Fibrinogen, A alpha polypeptide; Fibrinogen--alpha polypeptide chain; Fibrinopeptide A;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P02671 FIBA_HUMAN:

Detected in blood plasma (at protein level).

Sequence:
MFSMRIVCLVLSVVGTAWTADSGEGDFLAEGGGVRGPRVVERHQSACKDSDWPFCSDEDWNYKCPSGCRMKGLIDEVNQDFTNRINKLKNSLFEYQKNNKDSHSLTTNIMEILRGDFSSANNRDNTYNRVSEDLRSRIEVLKRKVIEKVQHIQLLQKNVRAQLVDMKRLEVDIDIKIRSCRGSCSRALAREVDLKDYEDQQKQLEQVIAKDLLPSRDRQHLPLIKMKPVPDLVPGNFKSQLQKVPPEWKALTDMPQMRMELERPGGNEITRGGSTSYGTGSETESPRNPSSAGSWNSGSSGPGSTGNRNPGSSGTGGTATWKPGSSGPGSTGSWNSGSSGTGSTGNQNPGSPRPGSTGTWNPGSSERGSAGHWTSESSVSGSTGQWHSESGSFRPDSPGSGNARPNNPDWGTFEEVSGNVSPGTRREYHTEKLVTSKGDKELRTGKEKVTSGSTTTTRRSCSKTVTKTVIGPDGHKEVTKEVVTSEDGSDCPEAMDLGTLSGIGTLDGFRHRHPDEAAFFDTASTGKTFPGFFSPMLGEFVSETESRGSESGIFTNTKESSSHHPGIAEFPSRGKSSSYSKQFTSSTSYNRGDSTFESKSYKMADEAGSEADHEGTHSTKRGHAKSRPVRDCDDVLQTHPSGTQSGIFNIKLPGSSKIFSVYCDQETSLGGWLLIQQRMDGSLNFNRTWQDYKRGFGSLNDEGEGEFWLGNDYLHLLTQRGSVLRVELEDWAGNEAYAEYHFRVGSEAEGYALQVSSYEGTAGDALIEGSVEEGAEYTSHNNMQFSTFDRDADQWEENCAEVYGGGWWYNNCQAANLNGIYYPGGSYDPRNNSPYEIENGVVWVSFRGADYSLRAVRMKIRPLVTQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Xenopus
91
Chicken
82
Zebrafish
64
Pig
0
Horse
0
Bovine
0
Sheep
0
Dog
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P02671 As Substrate

Site PTM Type Enzyme
S22 Phosphorylation
S45 Phosphorylation
K48 Acetylation
S50 Phosphorylation
S56 Phosphorylation
K63 Acetylation
S91 Phosphorylation
Y95 Phosphorylation
K100 Acetylation
T107 O-Glycosylation
T126 Phosphorylation
Y127 Phosphorylation
K142 Acetylation
K167 Acetylation
S179 Phosphorylation
S185 Phosphorylation
K238 Acetylation
S239 Phosphorylation
T252 O-Glycosylation
T252 Phosphorylation
S274 Phosphorylation
T275 Phosphorylation
S276 Phosphorylation
Y277 Phosphorylation
T279 Phosphorylation
S281 Phosphorylation
T283 O-Glycosylation
S291 Phosphorylation
S294 Phosphorylation
S297 Phosphorylation
S300 Phosphorylation
T320 O-Glycosylation
S351 O-Glycosylation
S356 Phosphorylation
S364 Phosphorylation
S365 Phosphorylation
S388 Phosphorylation
S397 Phosphorylation
S400 Phosphorylation
T412 Phosphorylation
S421 Phosphorylation
T424 Phosphorylation
T435 Phosphorylation
S451 Phosphorylation
S453 O-Glycosylation
S460 Phosphorylation
S485 Phosphorylation
S489 Phosphorylation
T499 Phosphorylation
S501 Phosphorylation
T505 Phosphorylation
S524 Phosphorylation
T525 O-Glycosylation
T525 Phosphorylation
S534 Phosphorylation
S542 Phosphorylation P68400 (CSNK2A1)
S549 Phosphorylation
S551 Phosphorylation
T555 Phosphorylation
T557 Phosphorylation
S560 Phosphorylation
S561 Phosphorylation
S562 O-Glycosylation
S572 Phosphorylation
S576 Phosphorylation
S577 Phosphorylation
S578 Phosphorylation
S586 O-Glycosylation
S594 Phosphorylation
K599 Acetylation
S609 Phosphorylation P68400 (CSNK2A1)
T616 O-Glycosylation
S618 Phosphorylation
K620 Acetylation
K625 Acetylation
S626 Phosphorylation
N686 N-Glycosylation

Research Backgrounds

Function:

Cleaved by the protease thrombin to yield monomers which, together with fibrinogen beta (FGB) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the immune response via both innate and T-cell mediated pathways.

PTMs:

The alpha chain is normally not N-glycosylated, even though glycosylation at Asn-686 was observed when a fragment of the protein was expressed in insect cells. It is well known that heterologous expression of isolated domains can lead to adventitious protein modifications. Besides, glycosylation at Asn-686 is supported by large-scale glycoproteomics studiesand, but the evidence is still quite tenuous. Most likely, Asn-686 is not glycosylated in the healthy human body, or only with low efficiency.

O-glycosylated.

Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.

About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.

Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.

Phosphorylated by FAM20C in the extracellular medium.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in blood plasma (at protein level).

Subunit Structure:

Heterohexamer; disulfide linked. Contains 2 sets of 3 non-identical chains (alpha, beta and gamma). The 2 heterotrimers are in head to head conformation with the N-termini in a small central domain.

(Microbial infection) Interacts with Staphylococcus aureus protein Fib; this interaction inhibits fibrinogen-dependent platelet aggregation and protects the bacteria form phagocytosis.

Family&Domains:

A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.

Research Fields

· Organismal Systems > Immune system > Complement and coagulation cascades.   (View pathway)

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

References

1). SIRT5-mediated SDHA desuccinylation promotes clear cell renal cell carcinoma tumorigenesis. FREE RADICAL BIOLOGY AND MEDICINE, 2019 (PubMed: 30703481) [IF=7.4]

Application: WB    Species: human    Sample: kidney

Fig. S2.| Verification of the differential succinylation of proteins found by MS/MS assay. These proteins were immunoprecipitated by corresponding antibodies and detected with pan anti-succinyllysine antibody. Adj, adjacent normal tissues. T, ccRCC tissues.

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