Hemoglobin subunit alpha Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from A2780, using HBA2 Antibody.

Product:	Hemoglobin subunit alpha Antibody
Catalog:	DF7893
Description:	Rabbit polyclonal antibody to Hemoglobin subunit alpha
Application:	WB IF/ICC
Reactivity:	Human
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken
Mol.Wt.:	15 kDa, 25 kDa; 15kD(Calculated).
Uniprot:	P69905
RRID:	AB_2841314

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:200-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(100%), Zebrafish(82%), Bovine(100%), Horse(100%), Sheep(100%), Dog(92%), Chicken(100%)

Clonality:

Polyclonal

Specificity:

Hemoglobin subunit alpha Antibody detects endogenous levels of total Hemoglobin subunit alpha.

RRID:

AB_2841314
Cite Format: Affinity Biosciences Cat# DF7893, RRID:AB_2841314.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Alpha 1 globin; Alpha globin; Alpha one globin; Alpha-globin; HBA_HUMAN; HBA1; HBA2; Hemoglobin alpha 1; Hemoglobin alpha 1 chain; Hemoglobin alpha 1 globin chain; Hemoglobin alpha 2; Hemoglobin alpha chain; Hemoglobin subunit alpha; MGC126895; MGC126897;

Immunogens

Immunogen:

Uniprot:

P69905(HBA_HUMAN) >>Visit HPA database.

Gene(ID):

HBA1; HBA2(3039)

Expression:

P69905 HBA_HUMAN:

Red blood cells.

Sequence:

P69905 HBA_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVLSPADKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHFDLSHGSAQVKGHGKKVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHLPAEFTPAVHASLDKFLASVSTVLTSKYR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Chicken

100

Dog

Zebrafish

Xenopus

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P69905 As Substrate

P69905

Site	PTM Type	Source
S4	O-Glycosylation	Uniprot
S4	Phosphorylation	Uniprot
K8	Acetylation	Uniprot
T9	Phosphorylation	Uniprot
K12	Acetylation	Uniprot
K17	Acetylation	Uniprot
K17	Ubiquitination	Uniprot
Y25	Phosphorylation	Uniprot
S36	O-Glycosylation	Uniprot
S36	Phosphorylation	Uniprot
T39	Phosphorylation	Uniprot
T40	Phosphorylation	Uniprot
K41	Acetylation	Uniprot
K41	Ubiquitination	Uniprot
T42	Phosphorylation	Uniprot
Y43	Phosphorylation	Uniprot
S50	Phosphorylation	Uniprot
S53	Phosphorylation	Uniprot
K57	Acetylation	Uniprot
K57	Ubiquitination	Uniprot
K61	Acetylation	Uniprot
K61	Ubiquitination	Uniprot
K62	Acetylation	Uniprot
S82	Phosphorylation	Uniprot
K91	Acetylation	Uniprot
K100	Acetylation	Uniprot
C105	S-Nitrosylation	Uniprot
T109	Phosphorylation	Uniprot
S132	Phosphorylation	Uniprot
S134	O-Glycosylation	Uniprot
T135	Phosphorylation	Uniprot
T138	Phosphorylation	Uniprot
S139	Phosphorylation	Uniprot
K140	Acetylation	Uniprot
Y141	Phosphorylation	Uniprot

Research Backgrounds

P69905_HBA_HUMAN

Function:

Involved in oxygen transport from the lung to the various peripheral tissues.

PTMs:

The initiator Met is not cleaved in variant Thionville and is acetylated.

Tissue Specificity:

Red blood cells.

Subunit Structure:

Heterotetramer of two alpha chains and two beta chains in adult hemoglobin A (HbA); two alpha chains and two delta chains in adult hemoglobin A2 (HbA2); two alpha chains and two epsilon chains in early embryonic hemoglobin Gower-2; two alpha chains and two gamma chains in fetal hemoglobin F (HbF).

(Microbial infection) Interacts with Staphylococcus aureus protein isdB.

Family&Domains:

Belongs to the globin family.

Research Fields

· Human Diseases > Infectious diseases: Parasitic > African trypanosomiasis.

· Human Diseases > Infectious diseases: Parasitic > Malaria.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

Hemoglobin subunit alpha Antibody - #DF7893